##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633518.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720518
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88000022206246	33.0	33.0	33.0	33.0	33.0
2	31.99498971573229	33.0	33.0	33.0	33.0	33.0
3	31.73431892055438	33.0	33.0	33.0	27.0	33.0
4	32.00158358292229	33.0	33.0	33.0	33.0	33.0
5	32.1049633735729	33.0	33.0	33.0	33.0	33.0
6	35.2660044579039	37.0	37.0	37.0	33.0	37.0
7	35.40083800821076	37.0	37.0	37.0	33.0	37.0
8	35.53813228815935	37.0	37.0	37.0	33.0	37.0
9	35.64597692215878	37.0	37.0	37.0	33.0	37.0
10	35.6364268484618	37.0	37.0	37.0	33.0	37.0
11	35.65840409261115	37.0	37.0	37.0	33.0	37.0
12	35.6657432569346	37.0	37.0	37.0	33.0	37.0
13	35.65301769005077	37.0	37.0	37.0	33.0	37.0
14	35.64319142616839	37.0	37.0	37.0	33.0	37.0
15	35.65824448521758	37.0	37.0	37.0	33.0	37.0
16	35.637156878801086	37.0	37.0	37.0	33.0	37.0
17	35.63792715796136	37.0	37.0	37.0	33.0	37.0
18	35.61679652694312	37.0	37.0	37.0	33.0	37.0
19	35.611901437576854	37.0	37.0	37.0	33.0	37.0
20	35.61420949927691	37.0	37.0	37.0	33.0	37.0
21	35.59449174066435	37.0	37.0	37.0	33.0	37.0
22	35.502696671006134	37.0	37.0	37.0	33.0	37.0
23	35.55258300278411	37.0	37.0	37.0	33.0	37.0
24	35.54344096885852	37.0	37.0	37.0	33.0	37.0
25	35.55820951038003	37.0	37.0	37.0	33.0	37.0
26	35.45901004555056	37.0	37.0	37.0	33.0	37.0
27	35.4629807999245	37.0	37.0	37.0	33.0	37.0
28	35.4768374974671	37.0	37.0	37.0	33.0	37.0
29	35.47553149262059	37.0	37.0	37.0	33.0	37.0
30	35.48114967287424	37.0	37.0	37.0	33.0	37.0
31	35.474428119769385	37.0	37.0	37.0	33.0	37.0
32	35.47007014397975	37.0	37.0	37.0	33.0	37.0
33	35.4478236490969	37.0	37.0	37.0	33.0	37.0
34	35.42908018953031	37.0	37.0	37.0	33.0	37.0
35	35.3529932631801	37.0	37.0	37.0	33.0	37.0
36	35.372551414399084	37.0	37.0	37.0	33.0	37.0
37	35.36263216186133	37.0	37.0	37.0	33.0	37.0
38	35.3265539514627	37.0	37.0	37.0	33.0	37.0
39	35.230026175612544	37.0	37.0	37.0	33.0	37.0
40	35.005224019386056	37.0	37.0	37.0	27.0	37.0
41	35.17264801156946	37.0	37.0	37.0	33.0	37.0
42	35.200316994162534	37.0	37.0	37.0	33.0	37.0
43	34.868619520955754	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	18.0
16	37.0
17	36.0
18	17.0
19	18.0
20	42.0
21	110.0
22	302.0
23	841.0
24	1650.0
25	2957.0
26	4773.0
27	7130.0
28	10102.0
29	13628.0
30	17671.0
31	22532.0
32	28909.0
33	37914.0
34	54719.0
35	110231.0
36	406878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.465029326123705	21.164495543483994	14.13288772799569	23.23758740239661
2	15.523831465695515	22.562517522116032	39.50935299326318	22.404298018925275
3	17.898373114897893	28.03524686406169	30.769252121390444	23.297127899649976
4	11.823021770448483	18.230911649674262	41.120554934089085	28.825511645788172
5	13.41520961308392	37.36797692771034	35.45199425968539	13.764819199520344
6	27.730049769748987	41.24255049839143	17.76943809870121	13.257961633158367
7	25.23601075892622	33.21415981280135	23.801764841405767	17.748064586866672
8	24.445884766237626	35.022580976464155	21.37115242089719	19.160381836401033
9	25.933703252382312	15.378380553990322	21.027788341165664	37.6601278524617
10	14.768402732478577	28.522396387043763	33.980691669049214	22.728509211428445
11	32.75615598777546	24.75496795361115	24.73331686370084	17.75555919491255
12	22.75501791766479	26.376579072278556	31.00824684463122	19.86015616542543
13	28.29519873202335	23.209829594819283	26.477895069935798	22.01707660322157
14	21.707438259696495	21.876760885918188	29.571363935390927	26.84443691899439
15	23.439664241559544	29.467966102165388	25.6805520472771	21.41181760899797
16	21.412095187073742	29.17692548971712	26.40600234831052	23.004976974898614
17	21.480795760827625	27.83497428239128	27.45469231858191	23.229537638199186
18	22.4839629266722	26.734654790026063	29.59690111836207	21.18448116493967
19	24.193288717283952	26.56130728170566	29.286430040609673	19.958973960400712
20	24.139299781546054	26.189075082093716	28.815518835060335	20.8561063012999
21	22.67799000163771	27.04970590602872	29.173872130883616	21.098431961449958
22	21.871625691516382	27.273572624139852	29.243405438864816	21.611396245478947
23	21.974052001476714	26.872888671761146	29.588018619937323	21.56504070682481
24	21.76295387485115	27.08384800934883	29.0421613339292	22.111036781870823
25	22.198196297663625	27.59056678667292	29.17067998301222	21.04055693265123
26	22.302426865116487	27.270935632420006	29.002884036207284	21.42375346625622
27	22.318248815435563	27.105082732145487	29.174149708959384	21.402518743459567
28	21.831515659567145	27.210562400939324	29.184558886800886	21.773363052692645
29	22.3219961194585	27.40694888954891	28.806636336635588	21.464418654357004
30	22.659531059598788	26.868031055435114	29.09295812179571	21.379479763170387
31	22.211242467224967	27.00598735909443	29.029392742443633	21.75337743123697
32	21.030564121923394	27.285924848511765	29.313355113959677	22.37015591560516
33	21.229587602252824	27.176003930505555	29.79120577140335	21.803202695838273
34	21.796957189133373	26.829864070016296	29.240907236182856	22.132271504667475
35	21.833181128021785	27.387518424244778	28.871312028290756	21.907988419442677
36	22.132965449856908	27.31520933550585	29.04951715293719	21.502308061700052
37	22.390696693212384	26.895650073974558	28.83828023727374	21.875372995539323
38	22.304092333571123	27.191270724673082	28.552513608265162	21.95212333349063
39	22.033731287767967	26.903561049134094	29.02911516436786	22.03359249873008
40	21.93713411739887	27.535606327669814	28.84841183703946	21.67884771789185
41	21.886614907608138	26.60252762595799	29.155135610768916	22.355721855664953
42	21.494952242692065	27.35365389900044	29.160687172284383	21.990706686023113
43	21.907294474253245	27.08995472701584	28.97915111072867	22.023599688002243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	106.0
2	124.0
3	423.5
4	723.0
5	723.0
6	866.0
7	1009.0
8	938.5
9	868.0
10	1192.0
11	1516.0
12	1516.0
13	2356.5
14	3197.0
15	5675.0
16	8153.0
17	9318.0
18	10483.0
19	10483.0
20	10190.5
21	9898.0
22	10667.5
23	11437.0
24	14046.5
25	16656.0
26	16656.0
27	19466.0
28	22276.0
29	24926.5
30	27577.0
31	30283.5
32	32990.0
33	32990.0
34	36308.0
35	39626.0
36	41257.0
37	42888.0
38	43951.5
39	45015.0
40	45015.0
41	46221.5
42	47428.0
43	47829.5
44	48231.0
45	48471.0
46	48711.0
47	48711.0
48	49384.0
49	50057.0
50	49005.0
51	47953.0
52	47505.5
53	47058.0
54	47058.0
55	44121.0
56	41184.0
57	36916.0
58	32648.0
59	29853.0
60	27058.0
61	27058.0
62	23560.0
63	20062.0
64	16616.5
65	13171.0
66	10971.5
67	8772.0
68	8772.0
69	7273.0
70	5774.0
71	4872.0
72	3970.0
73	2851.0
74	1732.0
75	1732.0
76	1270.0
77	808.0
78	631.0
79	454.0
80	381.0
81	308.0
82	308.0
83	255.5
84	203.0
85	197.5
86	192.0
87	157.0
88	122.0
89	122.0
90	99.5
91	77.0
92	48.0
93	19.0
94	10.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	720518.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.73261793789954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67673927563753	55.62924004957299
2	6.972982962631674	8.74866952164511
3	1.504699784359138	2.8318127005032485
4	0.688936092400579	1.7287505867277988
5	0.40604926619007153	1.2736266739933113
6	0.2708986131592741	1.019650751953655
7	0.19190985968398994	0.8427305534250153
8	0.14788770048897415	0.7421906089991467
9	0.11667110958475567	0.6587175727774157
>10	0.8382375337853649	10.56842448926281
>50	0.115869167543729	5.068634628914208
>100	0.06553967611257332	7.771109591997215
>500	0.0024605339154001923	1.053127076323202
>1k	8.947396056000698E-4	1.2577185856209008
>5k	2.2368490140001746E-4	0.8055966082840111
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5741	0.7967878665071518	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4210	0.5843018495027189	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2266	0.3144959598511071	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1275	0.17695602330545523	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.16821231391859745	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	975	0.13531931193946578	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	932	0.12935138331034063	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	851	0.11810947124152346	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	765	0.10617361398327316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.775780757732631E-4	0.0	0.0
4	0.0	0.0	2.775780757732631E-4	0.0	0.0
5	0.0	0.0	4.163671136598947E-4	0.0	0.0
6	0.0	0.0	9.715232652064209E-4	0.0	0.0
7	0.0	0.0	0.0019430465304128418	1.3878903788663155E-4	0.0
8	0.0	0.0	0.002220624606186105	1.3878903788663155E-4	0.0
9	0.0	0.0	0.0027757807577326312	1.3878903788663155E-4	0.0
10	0.0	0.0	0.0033309369092791577	4.163671136598947E-4	0.0
11	1.3878903788663155E-4	0.0	0.005829139591238526	4.163671136598947E-4	0.0
12	1.3878903788663155E-4	0.0	0.0062455067048984205	5.551561515465262E-4	0.0
13	1.3878903788663155E-4	0.0	0.006523084780671684	5.551561515465262E-4	0.0
14	1.3878903788663155E-4	0.0	0.007078240932218209	6.939451894331578E-4	0.0
15	1.3878903788663155E-4	0.0	0.007494608045878104	6.939451894331578E-4	0.0
16	1.3878903788663155E-4	0.0	0.007772186121651367	6.939451894331578E-4	0.0
17	1.3878903788663155E-4	0.0	0.008049764197424631	6.939451894331578E-4	0.0
18	1.3878903788663155E-4	0.0	0.008327342273197895	8.327342273197894E-4	0.0
19	1.3878903788663155E-4	0.0	0.008466131311084524	9.715232652064209E-4	0.0
20	1.3878903788663155E-4	0.0	0.008466131311084524	0.0011103123030930524	0.0
21	1.3878903788663155E-4	0.0	0.00888249842474442	0.0013878903788663156	0.0
22	1.3878903788663155E-4	0.0	0.009021287462631051	0.0016654684546395788	0.0
23	1.3878903788663155E-4	0.0	0.009021287462631051	0.002498202681959368	0.0
24	1.3878903788663155E-4	0.0	0.009021287462631051	0.0033309369092791577	0.0
25	1.3878903788663155E-4	0.0	0.009021287462631051	0.0038860930608256837	0.0
26	1.3878903788663155E-4	0.0	0.009021287462631051	0.004580038250258841	0.0
27	1.3878903788663155E-4	0.0	0.009021287462631051	0.006106717667011789	0.0
28	2.775780757732631E-4	0.0	0.009021287462631051	0.020263199531448207	0.0
29	2.775780757732631E-4	0.0	0.009021287462631051	0.04940889748764084	0.0
30	2.775780757732631E-4	0.0	0.009021287462631051	0.081746743315226	0.0
31	2.775780757732631E-4	0.0	0.009021287462631051	0.1446181774778701	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	45	1.3225424E-4	24.666668	22
GTATCAA	2580	0.0	23.591085	1
GGTATCA	910	0.0	23.37912	1
CGATTAC	40	0.0019304916	23.125	27
ACGTAGA	40	0.0019304916	23.125	26
GGACGTA	85	5.1739335E-8	21.764706	27
CCGCTCT	60	3.7238024E-5	21.583334	28
CGGGCGA	45	0.0038244424	20.555557	17
GTACTAG	45	0.0038244424	20.555557	1
TGCCGAG	45	0.0038244424	20.555557	11
TTGCCGA	55	5.1405333E-4	20.181818	10
GCCGCTC	65	6.897562E-5	19.923077	27
CGCTCTC	65	6.897562E-5	19.923077	29
GTCTTAC	75	9.257905E-6	19.733334	1
GACGTAT	85	1.2439887E-6	19.588236	28
AAACACG	85	1.2439887E-6	19.588236	15
CGGCGTG	60	9.2322554E-4	18.5	36
ACACGAA	90	2.1499472E-6	18.5	17
GCCGGCA	60	9.2322554E-4	18.5	15
CACGAGC	50	0.007032396	18.5	35
>>END_MODULE