FastQCFastQC Report
Fri 10 Feb 2017
ERR1633517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687341
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45670.6644445770003535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33570.4884038635844508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17750.2582415424076259No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13350.19422673752911582No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG12870.187243304269642No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12130.17647717799461984No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA11900.17313094955778863No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG11740.17080313847129736No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11220.16323775244020072No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10990.1598915240033695No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG9880.1437423345908363No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9380.13646792494555104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.13457657843777687No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9020.13123035000094568No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8720.12686570421377455No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8570.12468338132018897No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8520.12395594035566045No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8450.1229375230053205No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG8400.12221008204079198No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8300.12075520011173492No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8110.11799092444652653No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7680.11173493215158123No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7460.10853419190765573No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7460.10853419190765573No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7380.10737028636441011No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT7270.10576991624244735No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT7100.10329661696305036No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC7030.10227819961271044No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6990.10169624684108762No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6970.1014052704552762No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC6920.10067782949074768No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6900.10038685310493627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT950.027.26315928
CGCTCTC951.0913936E-1125.3157929
GGTATCA8000.024.9749981
CAACGGT400.00193040123.12500219
TCGGGTA400.00193040123.12500225
GTTCCGA502.7005715E-422.19999933
CTCTATC502.7005715E-422.1999992
GTATCAA19650.021.6539461
CGAGTCG603.7234815E-521.58333221
GCCGGCA1059.804353E-1021.14285915
GGGTTAG1500.020.9666677
GCCGCTC1151.3460522E-1020.91304427
AGTCCGG450.003824266620.5555555
ATTAGAC450.003824266620.5555553
CGGTTAC450.003824266620.55555522
AGTTCCG555.1402027E-420.18181832
GGTCTAG656.8969704E-519.9230771
ATAATAC951.6736703E-719.4736843
CCGCTGA801.615129E-518.526
GTACTAA609.2316675E-418.51
Produced by FastQC (version 0.11.5)