##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633517.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	687341
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83544848917786	33.0	33.0	33.0	27.0	33.0
2	31.95264941273691	33.0	33.0	33.0	33.0	33.0
3	31.66721321731135	33.0	33.0	33.0	27.0	33.0
4	31.953227000862746	33.0	33.0	33.0	33.0	33.0
5	32.060544911477706	33.0	33.0	33.0	33.0	33.0
6	35.207431245917235	37.0	37.0	37.0	33.0	37.0
7	35.347853539945966	37.0	37.0	37.0	33.0	37.0
8	35.491374732483585	37.0	37.0	37.0	33.0	37.0
9	35.60756305822001	37.0	37.0	37.0	33.0	37.0
10	35.60100736024768	37.0	37.0	37.0	33.0	37.0
11	35.62021325659316	37.0	37.0	37.0	33.0	37.0
12	35.60082404512462	37.0	37.0	37.0	33.0	37.0
13	35.60441905837132	37.0	37.0	37.0	33.0	37.0
14	35.604579095383514	37.0	37.0	37.0	33.0	37.0
15	35.615834061986696	37.0	37.0	37.0	33.0	37.0
16	35.6067061327638	37.0	37.0	37.0	33.0	37.0
17	35.59417668959075	37.0	37.0	37.0	33.0	37.0
18	35.57557893389162	37.0	37.0	37.0	33.0	37.0
19	35.55993167874461	37.0	37.0	37.0	33.0	37.0
20	35.55361167164479	37.0	37.0	37.0	33.0	37.0
21	35.558628104536176	37.0	37.0	37.0	33.0	37.0
22	35.45823834166738	37.0	37.0	37.0	33.0	37.0
23	35.51425711546379	37.0	37.0	37.0	33.0	37.0
24	35.50934106942551	37.0	37.0	37.0	33.0	37.0
25	35.51928227764676	37.0	37.0	37.0	33.0	37.0
26	35.41644685825522	37.0	37.0	37.0	33.0	37.0
27	35.41766604931177	37.0	37.0	37.0	33.0	37.0
28	35.44027782425317	37.0	37.0	37.0	33.0	37.0
29	35.44222300139232	37.0	37.0	37.0	33.0	37.0
30	35.446535271430044	37.0	37.0	37.0	33.0	37.0
31	35.43503152001699	37.0	37.0	37.0	33.0	37.0
32	35.42592250425917	37.0	37.0	37.0	33.0	37.0
33	35.42399769546702	37.0	37.0	37.0	33.0	37.0
34	35.403937783429186	37.0	37.0	37.0	33.0	37.0
35	35.32466854152451	37.0	37.0	37.0	33.0	37.0
36	35.34818816278965	37.0	37.0	37.0	33.0	37.0
37	35.34391517456401	37.0	37.0	37.0	33.0	37.0
38	35.32797403326733	37.0	37.0	37.0	33.0	37.0
39	35.22732966606095	37.0	37.0	37.0	33.0	37.0
40	35.00665172017965	37.0	37.0	37.0	27.0	37.0
41	35.1798510491881	37.0	37.0	37.0	33.0	37.0
42	35.20308696847707	37.0	37.0	37.0	33.0	37.0
43	34.87218571276848	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	23.0
16	32.0
17	25.0
18	20.0
19	24.0
20	49.0
21	143.0
22	333.0
23	799.0
24	1598.0
25	2930.0
26	4625.0
27	7178.0
28	10011.0
29	13222.0
30	17561.0
31	22092.0
32	28123.0
33	36341.0
34	52552.0
35	106443.0
36	383216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.364997868597975	20.89341389499535	13.277834437346236	23.463753799060438
2	16.606895267414572	21.8892805754349	38.747142975611816	22.756681181538713
3	17.706058564817173	27.681892975975536	29.64249186357281	24.969556595634483
4	11.899915762336308	18.134666781117378	40.08868960239532	29.876727854150996
5	13.769002576595895	37.02441728341536	35.40731601926846	13.799264120720284
6	28.92421665519735	40.63179702651231	17.19641342506849	13.247572893221852
7	27.31395333611701	32.013658431549985	23.129130955377317	17.54325727695569
8	24.453509975397946	35.22501931355761	20.618586698596474	19.702884012447967
9	26.0336572385468	14.845469715905205	20.54555162575781	38.57532141979018
10	15.56243553054452	27.6248616043565	33.66931406681691	23.143388798282075
11	33.861212993259535	24.211708598788665	23.646486969350004	18.2805914386018
12	23.437856900723222	26.058681207726586	29.575712783029097	20.9277491085211
13	27.615259383624725	22.879182239965314	26.17914543145251	23.326412944957454
14	22.24194395503833	21.32464089876786	27.939407077418632	28.49400806877518
15	23.984892506048674	28.72504331910944	25.776288625296612	21.51377554954528
16	23.40817730937046	28.36292320696714	25.03924544003631	23.18965404362609
17	22.80658363170537	27.112161212556796	25.72362189946475	24.357633256273086
18	22.416529786525174	26.902512726579676	28.72708015381012	21.953877333085035
19	25.60985013261249	25.932833920863153	27.67956516488904	20.777750781635316
20	24.54225777307043	26.043695923857303	28.140471760014318	21.273574543057958
21	22.60871968935361	27.18141359237991	28.095224932020642	22.114641786245837
22	23.005902455986185	26.4517903049578	27.81908834188561	22.7232188971704
23	22.206881300548055	26.671768452631227	29.003362232138052	22.11798801468267
24	22.82855234883413	27.80948612115384	26.40610701238541	22.955854517626623
25	22.752462023944446	27.36778396749212	28.336007891279586	21.543746117283852
26	23.686059757820356	26.937138916491232	27.597218847704415	21.779582477984
27	23.194746130377787	26.808381865769682	26.798343180459188	23.19852882339334
28	22.264785601324526	26.5078032592265	28.793568257968023	22.433842881480953
29	24.36432571314675	26.856247481235663	27.23699008206989	21.542436723547702
30	23.760404224395167	25.971388291983168	27.927186069214553	22.341021414407113
31	22.748679330928898	26.28898901709632	27.98145316516838	22.980878486806404
32	21.580554630088994	26.46779400617743	28.178444178362703	23.77320718537087
33	21.958241978872202	26.102618641984108	29.025621925652622	22.91351745349106
34	23.13509597128645	26.160086478181867	28.66975780580527	22.035059744726418
35	23.059442110975485	27.183595915273497	27.016720958010655	22.740241015740366
36	23.379516135368032	26.679479326855226	26.916770569484434	23.024233968292304
37	22.936504587970163	26.148156446363597	27.795373766441983	23.119965199224257
38	22.88718409057513	26.24621548838204	27.305805997314287	23.56079442372854
39	22.379721273720033	26.45222676953652	28.04590443462561	23.122147522117842
40	22.795962993623252	27.61656877736087	27.754782560621294	21.832685668394582
41	22.80891144279186	26.131861768758156	27.728885662284075	23.33034112616591
42	21.9402014429519	28.24173154227669	27.318608958289992	22.499458056481426
43	23.60633222810803	25.640693629508498	27.136457740772045	23.616516401611428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	74.0
2	99.0
3	331.0
4	563.0
5	563.0
6	673.5
7	784.0
8	714.0
9	644.0
10	873.5
11	1103.0
12	1103.0
13	1783.0
14	2463.0
15	4300.5
16	6138.0
17	6723.5
18	7309.0
19	7309.0
20	6429.5
21	5550.0
22	5713.0
23	5876.0
24	7183.5
25	8491.0
26	8491.0
27	10286.0
28	12081.0
29	14227.0
30	16373.0
31	19658.0
32	22943.0
33	22943.0
34	30626.5
35	38310.0
36	41448.0
37	44586.0
38	46902.5
39	49219.0
40	49219.0
41	50272.0
42	51325.0
43	53834.5
44	56344.0
45	53899.0
46	51454.0
47	51454.0
48	49318.5
49	47183.0
50	48481.0
51	49779.0
52	49288.0
53	48797.0
54	48797.0
55	47795.5
56	46794.0
57	38590.5
58	30387.0
59	28083.5
60	25780.0
61	25780.0
62	23185.0
63	20590.0
64	15833.5
65	11077.0
66	9260.5
67	7444.0
68	7444.0
69	6480.0
70	5516.0
71	5445.0
72	5374.0
73	4811.0
74	4248.0
75	4248.0
76	2644.0
77	1040.0
78	804.5
79	569.0
80	467.5
81	366.0
82	366.0
83	316.0
84	266.0
85	244.0
86	222.0
87	170.0
88	118.0
89	118.0
90	90.0
91	62.0
92	41.5
93	21.0
94	12.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	687341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.28670111706124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.14796738220036	36.42487913681525
2	8.328032477476741	7.2098610549142945
3	2.3764688914816343	3.0860849585867807
4	1.0966371399577999	1.8987921644488852
5	0.698751962255232	1.5123333672551138
6	0.5059150757164821	1.3139636803892862
7	0.3468824485546126	1.051077781133652
8	0.27025233139105564	0.9358665516090889
9	0.20348304701437364	0.79272988546501
>10	1.5644680438416159	13.807951504099755
>50	0.23297595695724455	7.1088637715211345
>100	0.2089223913206587	17.391154745298344
>500	0.01580662829070773	4.771370942086368
>1k	0.003436223541458202	2.6950704563770467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4567	0.6644445770003535	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3357	0.4884038635844508	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1775	0.2582415424076259	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1335	0.19422673752911582	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1287	0.187243304269642	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1213	0.17647717799461984	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1190	0.17313094955778863	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1174	0.17080313847129736	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1122	0.16323775244020072	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1099	0.1598915240033695	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	988	0.1437423345908363	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	938	0.13646792494555104	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	925	0.13457657843777687	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	902	0.13123035000094568	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	872	0.12686570421377455	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	857	0.12468338132018897	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	852	0.12395594035566045	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	845	0.1229375230053205	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	840	0.12221008204079198	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	830	0.12075520011173492	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	811	0.11799092444652653	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	768	0.11173493215158123	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	746	0.10853419190765573	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	746	0.10853419190765573	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	738	0.10737028636441011	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	727	0.10576991624244735	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	710	0.10329661696305036	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	703	0.10227819961271044	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	699	0.10169624684108762	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	697	0.1014052704552762	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	692	0.10067782949074768	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	690	0.10038685310493627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.4548819290570474E-4	0.0	0.0
5	0.0	0.0	1.4548819290570474E-4	0.0	0.0
6	0.0	0.0	7.274409645285237E-4	0.0	0.0
7	0.0	0.0	0.001745858314868457	1.4548819290570474E-4	0.0
8	0.0	0.0	0.0018913465077741616	1.4548819290570474E-4	0.0
9	0.0	0.0	0.002182322893585571	7.274409645285237E-4	0.0
10	1.4548819290570474E-4	0.0	0.00276427566520839	7.274409645285237E-4	0.0
11	1.4548819290570474E-4	0.0	0.0052375749446053705	7.274409645285237E-4	0.0
12	1.4548819290570474E-4	0.0	0.005819527716228189	7.274409645285237E-4	0.0
13	1.4548819290570474E-4	0.0	0.005965015909133895	8.729291574342285E-4	0.0
14	1.4548819290570474E-4	0.0	0.006546968680756713	8.729291574342285E-4	0.0
15	1.4548819290570474E-4	0.0	0.007274409645285237	8.729291574342285E-4	0.0
16	1.4548819290570474E-4	0.0	0.008147338802719466	0.0010184173503399333	0.0
17	1.4548819290570474E-4	0.0	0.008438315188530875	0.0010184173503399333	0.0
18	1.4548819290570474E-4	0.0	0.00858380338143658	0.001163905543245638	0.0
19	1.4548819290570474E-4	0.0	0.008729291574342284	0.0013093937361513426	0.0
20	1.4548819290570474E-4	0.0	0.009311244345965104	0.0014548819290570473	0.0
21	1.4548819290570474E-4	0.0	0.009893197117587921	0.0020368347006798665	0.0
22	1.4548819290570474E-4	0.0	0.009893197117587921	0.0029097638581140946	0.0
23	1.4548819290570474E-4	0.0	0.009893197117587921	0.0036372048226426183	0.0
24	1.4548819290570474E-4	0.0	0.010038685310493628	0.004801110365888256	0.0
25	1.4548819290570474E-4	0.0	0.010184173503399332	0.005383063137511075	0.0
26	1.4548819290570474E-4	0.0	0.010184173503399332	0.006110504102039599	0.0
27	1.4548819290570474E-4	0.0	0.010184173503399332	0.007856362416908055	0.0
28	1.4548819290570474E-4	0.0	0.010184173503399332	0.02182322893585571	0.0
29	1.4548819290570474E-4	0.0	0.010184173503399332	0.04641073353691981	0.0
30	1.4548819290570474E-4	0.0	0.010184173503399332	0.07885460055489196	0.0
31	1.4548819290570474E-4	0.0	0.010184173503399332	0.14999832688578157	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTCT	95	0.0	27.263159	28
CGCTCTC	95	1.0913936E-11	25.31579	29
GGTATCA	800	0.0	24.974998	1
CAACGGT	40	0.001930401	23.125002	19
TCGGGTA	40	0.001930401	23.125002	25
GTTCCGA	50	2.7005715E-4	22.199999	33
CTCTATC	50	2.7005715E-4	22.199999	2
GTATCAA	1965	0.0	21.653946	1
CGAGTCG	60	3.7234815E-5	21.583332	21
GCCGGCA	105	9.804353E-10	21.142859	15
GGGTTAG	150	0.0	20.966667	7
GCCGCTC	115	1.3460522E-10	20.913044	27
AGTCCGG	45	0.0038242666	20.555555	5
ATTAGAC	45	0.0038242666	20.555555	3
CGGTTAC	45	0.0038242666	20.555555	22
AGTTCCG	55	5.1402027E-4	20.181818	32
GGTCTAG	65	6.8969704E-5	19.923077	1
ATAATAC	95	1.6736703E-7	19.473684	3
CCGCTGA	80	1.615129E-5	18.5	26
GTACTAA	60	9.2316675E-4	18.5	1
>>END_MODULE