FastQCFastQC Report
Fri 10 Feb 2017
ERR1633516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644547
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35960.5579112151635179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25950.4026083435342962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13150.20401925693549114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.11186150893573316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG250.00549457529.59999830
GGTATCA5600.025.43751
CCGGCGT451.3223315E-424.66666623
GGCGTCA451.3223315E-424.66666625
GTATCAA16200.023.8672851
CGGCCTT802.7210262E-823.12524
TCGCTAT502.7003186E-422.19999911
CCTAGTA555.139726E-420.1818182
TCGGCGT555.139726E-420.18181811
GCGTCAA555.139726E-420.18181826
CGTCAAA555.139726E-420.18181827
TAACCTA1457.2759576E-1219.137935
GTTCTAG1501.2732926E-1118.51
CGAGTCG609.230816E-418.521
CGCTATC609.230816E-418.512
CGAATTA609.230816E-418.515
CATCGCT609.230816E-418.59
TGTCCGA1306.9485395E-1018.512
TGAACGT500.007031612618.4999985
GTATTAT1850.018.01
Produced by FastQC (version 0.11.5)