##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633516.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644547
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.846388238561346	33.0	33.0	33.0	33.0	33.0
2	31.978588062623828	33.0	33.0	33.0	33.0	33.0
3	31.69977829390254	33.0	33.0	33.0	27.0	33.0
4	31.97641754596639	33.0	33.0	33.0	33.0	33.0
5	32.082007983901875	33.0	33.0	33.0	33.0	33.0
6	35.24832479245113	37.0	37.0	37.0	33.0	37.0
7	35.39164095093143	37.0	37.0	37.0	33.0	37.0
8	35.508451672259746	37.0	37.0	37.0	33.0	37.0
9	35.61772842011521	37.0	37.0	37.0	33.0	37.0
10	35.630265907684	37.0	37.0	37.0	33.0	37.0
11	35.63828239057819	37.0	37.0	37.0	33.0	37.0
12	35.642429489238175	37.0	37.0	37.0	33.0	37.0
13	35.64678603732544	37.0	37.0	37.0	33.0	37.0
14	35.6205009099414	37.0	37.0	37.0	33.0	37.0
15	35.639942471223975	37.0	37.0	37.0	33.0	37.0
16	35.618724468502684	37.0	37.0	37.0	33.0	37.0
17	35.62283898614066	37.0	37.0	37.0	33.0	37.0
18	35.60427400949814	37.0	37.0	37.0	33.0	37.0
19	35.58998955855818	37.0	37.0	37.0	33.0	37.0
20	35.59444074675702	37.0	37.0	37.0	33.0	37.0
21	35.58445388776924	37.0	37.0	37.0	33.0	37.0
22	35.50012799687222	37.0	37.0	37.0	33.0	37.0
23	35.54597725224072	37.0	37.0	37.0	33.0	37.0
24	35.5379871444596	37.0	37.0	37.0	33.0	37.0
25	35.54836652718886	37.0	37.0	37.0	33.0	37.0
26	35.440084276243624	37.0	37.0	37.0	33.0	37.0
27	35.47224484793196	37.0	37.0	37.0	33.0	37.0
28	35.49459853199224	37.0	37.0	37.0	33.0	37.0
29	35.4907632802573	37.0	37.0	37.0	33.0	37.0
30	35.48794114315946	37.0	37.0	37.0	33.0	37.0
31	35.48323551269341	37.0	37.0	37.0	33.0	37.0
32	35.47192834657519	37.0	37.0	37.0	33.0	37.0
33	35.46775952723385	37.0	37.0	37.0	33.0	37.0
34	35.44114858962961	37.0	37.0	37.0	33.0	37.0
35	35.38350034985812	37.0	37.0	37.0	33.0	37.0
36	35.39851399510044	37.0	37.0	37.0	33.0	37.0
37	35.39845348748811	37.0	37.0	37.0	33.0	37.0
38	35.38541332129387	37.0	37.0	37.0	33.0	37.0
39	35.27773459499463	37.0	37.0	37.0	33.0	37.0
40	35.077874848537036	37.0	37.0	37.0	33.0	37.0
41	35.22872653196741	37.0	37.0	37.0	33.0	37.0
42	35.281293683781016	37.0	37.0	37.0	33.0	37.0
43	34.94019520686622	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	11.0
16	31.0
17	23.0
18	22.0
19	23.0
20	40.0
21	120.0
22	310.0
23	693.0
24	1474.0
25	2681.0
26	4326.0
27	6344.0
28	8867.0
29	12242.0
30	15686.0
31	20049.0
32	25574.0
33	33820.0
34	48856.0
35	99237.0
36	364113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53916937011576	21.447000761775325	14.204549862151246	23.80928000595767
2	15.42618304018171	22.490679500486387	39.65125894620563	22.431878513126275
3	17.324725737611065	27.92084983717246	31.161265198658906	23.593159226557567
4	12.189646371792904	18.36049194240296	40.50395083678925	28.94591084901489
5	12.98276153639688	38.18464751212867	35.801888768390825	13.030702183083623
6	27.843431122943713	41.03409060937371	18.115203390908654	13.00727487677392
7	25.04596251320695	33.20502616566364	24.269913598232556	17.479097722896856
8	23.670112497614603	36.38865746020073	21.30705751481273	18.634172527371938
9	25.374410244714507	16.02877679982996	20.879160092281865	37.717652863173676
10	14.384521221881414	28.779127045816676	34.5901850446903	22.24616668761161
11	32.26343462928227	24.6885021573291	25.08459429645937	17.963468916929255
12	21.969693443612336	26.598060343155737	30.940645135265544	20.491601077966386
13	26.989342902844943	23.031679613744227	27.485505323894145	22.493472159516685
14	21.365082763553318	22.039199623921917	28.768266705143304	27.82745090738146
15	23.124923395811322	29.514527257127877	25.705495487528452	21.65505385953235
16	22.014841431268785	29.643299867969287	25.897413221999326	22.444445478762603
17	21.614560303593066	28.436871166881545	27.24347487460185	22.705093654923537
18	21.58865063370088	28.246815205097537	28.978647018758913	21.185887142442677
19	23.759167291136254	27.167297342164343	28.94218730364116	20.13134806305824
20	23.481608013069643	26.74079624914863	29.011383188502933	20.766212549278794
21	21.854108389302876	27.956068370498972	28.41127179243717	21.778551447760986
22	21.45150004576858	28.028832653010564	28.438267496396698	22.081399804824166
23	21.565533622838988	27.83024356641176	29.016968506563522	21.58725430418573
24	22.21529229055445	28.175602399824996	27.901456371684297	21.707648937936256
25	22.314897129301663	27.496520812291426	28.702639217931353	21.48594284047556
26	22.074728452696235	27.73994759109886	28.627547719561182	21.55777623664372
27	22.07457330497233	27.707211421354845	28.05148422070074	22.16673105297209
28	21.374701922435445	27.75809987479579	29.006728756785773	21.860469445982993
29	22.71347163201442	27.864376065670925	28.041865061818612	21.38028724049604
30	22.017789238022985	27.739327000203247	28.751045307789813	21.49183845398396
31	22.204276802157175	27.668424490378513	28.513824437938585	21.613474269525728
32	21.094039689890728	27.687662808142772	28.889437077513353	22.328860424453143
33	21.32552009395746	27.584489571745735	29.252793046899605	21.837197287397196
34	21.740540255404184	27.582627799058873	28.638252912510648	22.038579033026295
35	21.9977751816392	28.252400523158126	28.216716546659903	21.533107748542776
36	22.345150935463202	27.827140611933654	28.18863480863304	21.639073643970104
37	22.402245297860357	27.084138162151095	28.23161072815481	22.282005811833738
38	22.469889705483077	27.59922860551674	28.1552780479934	21.775603641006786
39	21.773586720596015	27.635688320634493	28.544078244100117	22.04664671466937
40	21.830991378440984	28.0066465284921	28.48403607494876	21.678326018118153
41	21.623248576131765	27.48767739202882	28.740495262564252	22.148578769275165
42	21.856125309713644	27.684870149112477	28.384586383925452	22.074418157248424
43	21.80104786772726	27.237579261093448	28.606137333662247	22.355235537517046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	57.0
2	74.0
3	251.0
4	428.0
5	428.0
6	496.5
7	565.0
8	522.5
9	480.0
10	719.0
11	958.0
12	958.0
13	1495.0
14	2032.0
15	3793.5
16	5555.0
17	6431.5
18	7308.0
19	7308.0
20	7357.0
21	7406.0
22	8553.0
23	9700.0
24	11552.5
25	13405.0
26	13405.0
27	15988.0
28	18571.0
29	21886.0
30	25201.0
31	27444.0
32	29687.0
33	29687.0
34	33467.0
35	37247.0
36	39244.0
37	41241.0
38	43710.0
39	46179.0
40	46179.0
41	48232.5
42	50286.0
43	48917.0
44	47548.0
45	47177.5
46	46807.0
47	46807.0
48	45257.0
49	43707.0
50	44396.0
51	45085.0
52	44319.5
53	43554.0
54	43554.0
55	38980.0
56	34406.0
57	30057.0
58	25708.0
59	22641.5
60	19575.0
61	19575.0
62	17139.0
63	14703.0
64	12068.0
65	9433.0
66	7920.5
67	6408.0
68	6408.0
69	5322.0
70	4236.0
71	3572.5
72	2909.0
73	2366.5
74	1824.0
75	1824.0
76	1386.5
77	949.0
78	778.0
79	607.0
80	446.5
81	286.0
82	286.0
83	226.5
84	167.0
85	155.0
86	143.0
87	109.5
88	76.0
89	76.0
90	57.5
91	39.0
92	25.5
93	12.0
94	7.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	644547.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.459566629803795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.18500987723455	49.22427874283825
2	7.183809928656229	8.111895906456247
3	1.9100908792265454	3.235287097940149
4	0.8867498212032485	2.0026204245676564
5	0.5216832169562615	1.4727004173696219
6	0.34546350641879586	1.1702831915290592
7	0.2392014253800537	0.9453646169932427
8	0.18517667201210686	0.8363995721402296
9	0.15002725473327136	0.7623426406409712
>10	1.1141771139057832	12.998048826349088
>50	0.17374797652876475	6.960188153437839
>100	0.10123676854046555	10.189841621906885
>500	0.0027888916953293964	0.9088569064135065
>1k	8.366675085988188E-4	1.1818918814171484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3596	0.5579112151635179	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2595	0.4026083435342962	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1315	0.20401925693549114	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	721	0.11186150893573316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.5514772390531645E-4	0.0	0.0
2	0.0	0.0	3.102954478106329E-4	0.0	0.0
3	0.0	0.0	3.102954478106329E-4	0.0	0.0
4	0.0	0.0	3.102954478106329E-4	0.0	0.0
5	0.0	0.0	4.6544317171594936E-4	0.0	0.0
6	0.0	0.0	0.0018617726868637974	0.0	0.0
7	0.0	0.0	0.003102954478106329	3.102954478106329E-4	0.0
8	0.0	0.0	0.003878693097632911	3.102954478106329E-4	0.0
9	1.5514772390531645E-4	0.0	0.00480957944106481	0.001396329515147848	0.0
10	1.5514772390531645E-4	0.0	0.0054301703366860755	0.0018617726868637974	0.0
11	1.5514772390531645E-4	0.0	0.007136795299644556	0.0020169204107691138	0.0
12	1.5514772390531645E-4	0.0	0.007757386195265822	0.0020169204107691138	0.0
13	1.5514772390531645E-4	0.0	0.008377977090887088	0.0020169204107691138	0.0
14	1.5514772390531645E-4	0.0	0.00868827253869772	0.0020169204107691138	0.0
15	1.5514772390531645E-4	0.0	0.009464011158224303	0.0020169204107691138	0.0
16	1.5514772390531645E-4	0.0	0.009774306606034936	0.0020169204107691138	0.0
17	1.5514772390531645E-4	0.0	0.009774306606034936	0.0020169204107691138	0.0
18	1.5514772390531645E-4	0.0	0.009929454329940253	0.0020169204107691138	0.0
19	1.5514772390531645E-4	0.0	0.010394897501656201	0.0020169204107691138	0.0
20	1.5514772390531645E-4	0.0	0.010705192949466836	0.002327215858579747	0.0
21	1.5514772390531645E-4	0.0	0.011325783845088101	0.003102954478106329	0.0
22	1.5514772390531645E-4	0.0	0.011636079292898734	0.003568397649822278	0.0
23	1.5514772390531645E-4	0.0	0.011636079292898734	0.004188988545443544	0.0
24	1.5514772390531645E-4	0.0	0.011636079292898734	0.004499283993254177	0.0
25	1.5514772390531645E-4	0.0	0.011636079292898734	0.004499283993254177	0.0
26	3.102954478106329E-4	0.0	0.011636079292898734	0.00480957944106481	0.0
27	3.102954478106329E-4	0.0	0.011636079292898734	0.006516204404023291	0.0
28	3.102954478106329E-4	0.0	0.011636079292898734	0.015359624666626328	0.0
29	3.102954478106329E-4	0.0	0.01179122701680405	0.03413249925916962	0.0
30	3.102954478106329E-4	0.0	0.01179122701680405	0.05833554418839899	0.0
31	3.102954478106329E-4	0.0	0.01179122701680405	0.11651594065289266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCG	25	0.005494575	29.599998	30
GGTATCA	560	0.0	25.4375	1
CCGGCGT	45	1.3223315E-4	24.666666	23
GGCGTCA	45	1.3223315E-4	24.666666	25
GTATCAA	1620	0.0	23.867285	1
CGGCCTT	80	2.7210262E-8	23.125	24
TCGCTAT	50	2.7003186E-4	22.199999	11
CCTAGTA	55	5.139726E-4	20.181818	2
TCGGCGT	55	5.139726E-4	20.181818	11
GCGTCAA	55	5.139726E-4	20.181818	26
CGTCAAA	55	5.139726E-4	20.181818	27
TAACCTA	145	7.2759576E-12	19.13793	5
GTTCTAG	150	1.2732926E-11	18.5	1
CGAGTCG	60	9.230816E-4	18.5	21
CGCTATC	60	9.230816E-4	18.5	12
CGAATTA	60	9.230816E-4	18.5	15
CATCGCT	60	9.230816E-4	18.5	9
TGTCCGA	130	6.9485395E-10	18.5	12
TGAACGT	50	0.0070316126	18.499998	5
GTATTAT	185	0.0	18.0	1
>>END_MODULE