FastQCFastQC Report
Fri 10 Feb 2017
ERR1633515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455633
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27030.5932406125105073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.4253423259509298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10550.23154600303314288No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7090.1556076930336477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.11719958826511688No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5210.11434641476802603No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4790.10512846962357862No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4600.10095844682013813No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4590.10073897193574653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG251.2324561E-437.028
TAGTACT601.3344488E-624.6666664
GGTATCA4950.023.5454541
CCCACGC400.00192940823.12519
CTACGGT400.00192940823.12527
TGGGCGT400.00192940823.12534
TTCGCAC400.00192940823.12527
GTCTTAC502.6986343E-422.21
GTATCAA13000.022.0576931
TCTATAC957.1158865E-921.4210533
AGCGTCA450.003822316920.55555537
TCGCACC450.003822316920.55555528
GCGCGGT450.003822316920.55555525
TGCGTTT450.003822316920.55555524
TTACACT759.245654E-619.7333344
CTTAAGC500.007028524318.531
CCCTAGT500.007028524318.51
TTTCGCA500.007028524318.526
CGTTAAT500.007028524318.518
CGCACCT500.007028524318.529