##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633515.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455633
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875581882787245	33.0	33.0	33.0	33.0	33.0
2	31.9997651618737	33.0	33.0	33.0	33.0	33.0
3	31.715194026771545	33.0	33.0	33.0	27.0	33.0
4	31.992601501647158	33.0	33.0	33.0	33.0	33.0
5	32.09464195964735	33.0	33.0	33.0	33.0	33.0
6	35.25725529099078	37.0	37.0	37.0	33.0	37.0
7	35.40085990259705	37.0	37.0	37.0	33.0	37.0
8	35.54319155987385	37.0	37.0	37.0	33.0	37.0
9	35.6485987626006	37.0	37.0	37.0	33.0	37.0
10	35.65479453858698	37.0	37.0	37.0	33.0	37.0
11	35.66590435723488	37.0	37.0	37.0	33.0	37.0
12	35.678041318341734	37.0	37.0	37.0	33.0	37.0
13	35.66323554264068	37.0	37.0	37.0	33.0	37.0
14	35.645559035451775	37.0	37.0	37.0	33.0	37.0
15	35.65187552262457	37.0	37.0	37.0	33.0	37.0
16	35.63753722842726	37.0	37.0	37.0	33.0	37.0
17	35.63088274993251	37.0	37.0	37.0	33.0	37.0
18	35.61690439454561	37.0	37.0	37.0	33.0	37.0
19	35.613783461689565	37.0	37.0	37.0	33.0	37.0
20	35.627685000866926	37.0	37.0	37.0	33.0	37.0
21	35.618471445220166	37.0	37.0	37.0	33.0	37.0
22	35.52543823647541	37.0	37.0	37.0	33.0	37.0
23	35.57120533411759	37.0	37.0	37.0	33.0	37.0
24	35.55945245405842	37.0	37.0	37.0	33.0	37.0
25	35.57704775554009	37.0	37.0	37.0	33.0	37.0
26	35.47254259458819	37.0	37.0	37.0	33.0	37.0
27	35.48431083788927	37.0	37.0	37.0	33.0	37.0
28	35.49644340949843	37.0	37.0	37.0	33.0	37.0
29	35.49062732506206	37.0	37.0	37.0	33.0	37.0
30	35.499494110391474	37.0	37.0	37.0	33.0	37.0
31	35.48367875022222	37.0	37.0	37.0	33.0	37.0
32	35.48955189812854	37.0	37.0	37.0	33.0	37.0
33	35.474173292979216	37.0	37.0	37.0	33.0	37.0
34	35.45082336002879	37.0	37.0	37.0	33.0	37.0
35	35.39014074924336	37.0	37.0	37.0	33.0	37.0
36	35.40104206675109	37.0	37.0	37.0	33.0	37.0
37	35.40690863041088	37.0	37.0	37.0	33.0	37.0
38	35.39219503416127	37.0	37.0	37.0	33.0	37.0
39	35.28927009237698	37.0	37.0	37.0	33.0	37.0
40	35.069700833785085	37.0	37.0	37.0	33.0	37.0
41	35.21085610568154	37.0	37.0	37.0	33.0	37.0
42	35.27554193835828	37.0	37.0	37.0	33.0	37.0
43	34.922830435899066	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	12.0
16	24.0
17	24.0
18	18.0
19	25.0
20	27.0
21	86.0
22	216.0
23	433.0
24	955.0
25	1817.0
26	2999.0
27	4399.0
28	6197.0
29	8505.0
30	10952.0
31	14160.0
32	18114.0
33	23817.0
34	34388.0
35	69881.0
36	258584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26354544117744	21.988530242541696	14.496974538718662	23.250949777562205
2	15.064756064639742	22.828680099992756	40.458219663632796	21.648344171734706
3	17.27881869838225	28.457552459984242	31.63774353481859	22.625885306814915
4	12.095480353705723	18.472981544356973	41.11774169122956	28.31379641070774
5	12.609710007835254	38.501162119512855	36.11920119921077	12.769926673441127
6	27.219055687362413	41.5402308436834	18.20236901190212	13.038344457052057
7	24.25855897180406	33.946838793502664	24.68214549867986	17.112456736013414
8	23.227685439816696	36.55420042007493	21.76181268696517	18.456301453143208
9	25.063373372868075	16.02430025919984	21.452792049741788	37.45953431819029
10	13.913390821121386	29.044208825963004	35.28739138736658	21.755008965549028
11	31.7290450867255	25.150724376855933	25.504078940726416	17.61615159569215
12	21.489005405666404	27.05159635057162	31.799496524615208	19.65990171914677
13	26.836730438752245	23.47174151126016	27.860580774439075	21.830947275548525
14	21.063443604831082	22.610083115138718	29.54285576330073	26.783617516729475
15	22.599767795572316	30.029212107112524	26.16623466693589	21.204785430379275
16	21.354028351765567	30.29499619211076	26.243709301126128	22.107266154997554
17	21.08670794257659	28.896063278998668	27.707826254902518	22.30940252352222
18	21.31627867165021	28.20603424247146	29.652373730612137	20.82531335526619
19	23.131116490684388	27.63298531932498	29.726117291767718	19.50978089822291
20	23.055178180684894	27.13214363314334	29.64798423292431	20.164693953247458
21	21.425138214308443	28.484328395880016	28.956199397321967	21.13433399248957
22	21.007258034426833	28.397635816545336	29.070765286974385	21.52434086205345
23	21.038203993126046	28.01531056793516	29.710973524744695	21.2355119141941
24	21.749083143670454	28.283728351546088	28.659469353624516	21.30771915115894
25	21.7086997649424	27.759183377850157	29.520030375324	21.012086481883447
26	21.44115988086903	27.974268764553926	29.188623299892676	21.395948054684364
27	21.50107652430794	27.972293490594403	28.91010967159973	21.616520313497926
28	20.772419908127812	28.343425520100606	29.53934416515046	21.344810406621118
29	22.108583004303902	28.235004927211154	28.748795631571898	20.90761643691304
30	21.290600110176392	28.4612835330189	29.336329897088227	20.911786459716485
31	21.61476451442279	28.01223791955368	29.31504083330224	21.05795673272129
32	20.60781374483411	28.136241229234933	29.33786622127897	21.918078804651987
33	20.879523651710915	27.777180318370263	30.002216696332358	21.34107933358646
34	21.059712531796425	27.786178788630323	29.401513937752533	21.75259474182072
35	21.291697484598348	28.56136408030147	28.965636817350806	21.181301617749373
36	21.794953394508298	28.155555019061396	28.815077046658168	21.234414539772143
37	21.842359969536886	27.515346781291083	28.766353622323233	21.8759396268488
38	21.846091042571544	28.25629399099714	28.778205266080377	21.119409700350943
39	21.494492277776192	27.83270746412134	29.22505613070168	21.44774412740078
40	21.442915679944168	28.138874927847628	29.170406884488177	21.247802507720028
41	21.063004655062297	28.023870088426435	29.39558811587396	21.51753714063731
42	21.278967941303637	27.91149894761793	29.244589395412536	21.564943715665898
43	21.22080709693986	27.722750547041148	29.055402045066973	22.001040310952018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	51.5
2	58.0
3	214.0
4	370.0
5	370.0
6	408.5
7	447.0
8	431.5
9	416.0
10	581.0
11	746.0
12	746.0
13	1195.5
14	1645.0
15	3105.5
16	4566.0
17	5277.5
18	5989.0
19	5989.0
20	6017.0
21	6045.0
22	7138.5
23	8232.0
24	9895.5
25	11559.0
26	11559.0
27	13406.5
28	15254.0
29	18322.5
30	21391.0
31	22656.5
32	23922.0
33	23922.0
34	25847.0
35	27772.0
36	28941.5
37	30111.0
38	31167.5
39	32224.0
40	32224.0
41	33525.5
42	34827.0
43	33263.0
44	31699.0
45	31626.0
46	31553.0
47	31553.0
48	30343.5
49	29134.0
50	29337.0
51	29540.0
52	29333.5
53	29127.0
54	29127.0
55	25981.5
56	22836.0
57	20030.0
58	17224.0
59	14894.5
60	12565.0
61	12565.0
62	11028.0
63	9491.0
64	7686.5
65	5882.0
66	4891.5
67	3901.0
68	3901.0
69	3231.0
70	2561.0
71	2199.0
72	1837.0
73	1512.5
74	1188.0
75	1188.0
76	882.5
77	577.0
78	437.0
79	297.0
80	238.5
81	180.0
82	180.0
83	157.0
84	134.0
85	131.5
86	129.0
87	115.0
88	101.0
89	101.0
90	69.5
91	38.0
92	26.0
93	14.0
94	10.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	455633.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.7473350413954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84711151533939	56.376668087546165
2	5.790178065240094	7.266364860179175
3	1.3849395887623717	2.607038051644947
4	0.6596206853173445	1.6555776056736908
5	0.3721032982946629	1.1674245164051753
6	0.2717290427285078	1.0230163970737995
7	0.19368220423623816	0.8507129512537046
8	0.14807072067955224	0.7432834496240592
9	0.1341100288953343	0.7573542223956085
>10	0.9868657240847916	12.62698117365321
>50	0.144007984054306	6.223375967700298
>100	0.06545817454432269	7.0459512557903174
>500	0.0010614839115295571	0.39163908543024073
>1k	0.0010614839115295571	1.2646123756296208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2703	0.5932406125105073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1938	0.4253423259509298	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1055	0.23154600303314288	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	709	0.1556076930336477	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.11719958826511688	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	521	0.11434641476802603	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	479	0.10512846962357862	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	460	0.10095844682013813	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	459	0.10073897193574653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.1947488439160465E-4	0.0	0.0
4	0.0	0.0	2.1947488439160465E-4	0.0	0.0
5	2.1947488439160465E-4	0.0	2.1947488439160465E-4	0.0	0.0
6	2.1947488439160465E-4	0.0	4.389497687832093E-4	0.0	0.0
7	2.1947488439160465E-4	0.0	0.0010973744219580231	0.0	0.0
8	4.389497687832093E-4	0.0	0.0017557990751328372	0.0	0.0
9	4.389497687832093E-4	0.0	0.0021947488439160463	0.0010973744219580231	0.0
10	6.584246531748139E-4	0.0	0.0028531734970908603	0.0010973744219580231	0.0
11	8.778995375664186E-4	0.0	0.0048284474566153025	0.0010973744219580231	0.0
12	8.778995375664186E-4	0.0	0.0052673972253985115	0.0010973744219580231	0.0
13	8.778995375664186E-4	0.0	0.0052673972253985115	0.0010973744219580231	0.0
14	8.778995375664186E-4	0.0	0.0054868721097901165	0.0010973744219580231	0.0
15	8.778995375664186E-4	0.0	0.005706346994181721	0.0010973744219580231	0.0
16	8.778995375664186E-4	0.0	0.00658424653174814	0.0010973744219580231	0.0
17	8.778995375664186E-4	0.0	0.006803721416139744	0.0010973744219580231	0.0
18	8.778995375664186E-4	0.0	0.007462146069314558	0.0010973744219580231	0.0
19	8.778995375664186E-4	0.0	0.007462146069314558	0.0015363241907412324	0.0
20	8.778995375664186E-4	0.0	0.007462146069314558	0.0015363241907412324	0.0
21	8.778995375664186E-4	0.0	0.007462146069314558	0.0017557990751328372	0.0
22	8.778995375664186E-4	0.0	0.007462146069314558	0.0021947488439160463	0.0
23	8.778995375664186E-4	0.0	0.007462146069314558	0.0024142237283076512	0.0
24	8.778995375664186E-4	0.0	0.007462146069314558	0.003731073034657279	0.0
25	8.778995375664186E-4	0.0	0.007462146069314558	0.0039505479190488834	0.0
26	8.778995375664186E-4	0.0	0.007462146069314558	0.0046089725722236975	0.0
27	8.778995375664186E-4	0.0	0.007462146069314558	0.00614529676296493	0.0
28	8.778995375664186E-4	0.0	0.007462146069314558	0.018216415404503184	0.0
29	0.0010973744219580231	0.0	0.007462146069314558	0.04060285361244686	0.0
30	0.0010973744219580231	0.0	0.007462146069314558	0.06869563881457226	0.0
31	0.0010973744219580231	0.0	0.007462146069314558	0.13058755621300477	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTG	25	1.2324561E-4	37.0	28
TAGTACT	60	1.3344488E-6	24.666666	4
GGTATCA	495	0.0	23.545454	1
CCCACGC	40	0.001929408	23.125	19
CTACGGT	40	0.001929408	23.125	27
TGGGCGT	40	0.001929408	23.125	34
TTCGCAC	40	0.001929408	23.125	27
GTCTTAC	50	2.6986343E-4	22.2	1
GTATCAA	1300	0.0	22.057693	1
TCTATAC	95	7.1158865E-9	21.421053	3
AGCGTCA	45	0.0038223169	20.555555	37
TCGCACC	45	0.0038223169	20.555555	28
GCGCGGT	45	0.0038223169	20.555555	25
TGCGTTT	45	0.0038223169	20.555555	24
TTACACT	75	9.245654E-6	19.733334	4
CTTAAGC	50	0.0070285243	18.5	31
CCCTAGT	50	0.0070285243	18.5	1
TTTCGCA	50	0.0070285243	18.5	26
CGTTAAT	50	0.0070285243	18.5	18
CGCACCT	50	0.0070285243	18.5	29
>>END_MODULE