FastQCFastQC Report
Fri 10 Feb 2017
ERR1633514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387520
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35470.9153075970272502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.6892547481420314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14280.36849710982658956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.1953447563996697No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5680.14657308009909165No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4890.12618703550784477No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4330.11173616845582164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA251.2321162E-436.99999632
GGACCGT405.9301266E-527.756
GGTATCA4100.026.621951
TCTTTCG358.858746E-426.4285710
TGGACCG509.0708E-625.8999985
CTGCTCG951.0913936E-1125.315799
CGAGTCG451.3210022E-424.66666621
ATAAGAC451.3210022E-424.6666663
ATTATAC451.3210022E-424.6666663
GTATCAA12650.023.984191
TCACGGT400.001928890123.1251
TTTCGAG400.001928890123.12512
GCCGGCA802.7139322E-823.12515
GCTTCGC802.7139322E-823.12522
TGCTCGC802.7139322E-823.12510
TTCGCCG802.7139322E-823.12524
GCCGCTC753.7275458E-722.227
AGCTTCG855.158472E-821.76470621
ACTGCTC1450.021.6896578
CCGCTCT705.087535E-621.14285728
Produced by FastQC (version 0.11.5)