##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633514.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387520
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.771428571428572	33.0	33.0	33.0	27.0	33.0
2	31.926672171758877	33.0	33.0	33.0	33.0	33.0
3	31.643130677126344	33.0	33.0	33.0	27.0	33.0
4	31.921193744838977	33.0	33.0	33.0	33.0	33.0
5	32.033575041288195	33.0	33.0	33.0	33.0	33.0
6	35.17000928984311	37.0	37.0	37.0	33.0	37.0
7	35.32155759702725	37.0	37.0	37.0	33.0	37.0
8	35.44825041288192	37.0	37.0	37.0	33.0	37.0
9	35.56503406275805	37.0	37.0	37.0	33.0	37.0
10	35.571299545829895	37.0	37.0	37.0	33.0	37.0
11	35.57899463253509	37.0	37.0	37.0	33.0	37.0
12	35.57968104872007	37.0	37.0	37.0	33.0	37.0
13	35.57113439306358	37.0	37.0	37.0	33.0	37.0
14	35.555517134599505	37.0	37.0	37.0	33.0	37.0
15	35.565462427745665	37.0	37.0	37.0	33.0	37.0
16	35.546973059455	37.0	37.0	37.0	33.0	37.0
17	35.56297223369116	37.0	37.0	37.0	33.0	37.0
18	35.53256606110652	37.0	37.0	37.0	33.0	37.0
19	35.531954479768785	37.0	37.0	37.0	33.0	37.0
20	35.527694054500415	37.0	37.0	37.0	33.0	37.0
21	35.51907514450867	37.0	37.0	37.0	33.0	37.0
22	35.41571015689513	37.0	37.0	37.0	33.0	37.0
23	35.46992929397192	37.0	37.0	37.0	33.0	37.0
24	35.46649721304707	37.0	37.0	37.0	33.0	37.0
25	35.484372419488025	37.0	37.0	37.0	33.0	37.0
26	35.36360962014864	37.0	37.0	37.0	33.0	37.0
27	35.372125309661435	37.0	37.0	37.0	33.0	37.0
28	35.38739419900908	37.0	37.0	37.0	33.0	37.0
29	35.39490606936416	37.0	37.0	37.0	33.0	37.0
30	35.38077776630884	37.0	37.0	37.0	33.0	37.0
31	35.36555016515277	37.0	37.0	37.0	33.0	37.0
32	35.35873761354253	37.0	37.0	37.0	33.0	37.0
33	35.3378999793559	37.0	37.0	37.0	33.0	37.0
34	35.31491019818332	37.0	37.0	37.0	33.0	37.0
35	35.238240606936415	37.0	37.0	37.0	33.0	37.0
36	35.24684919488026	37.0	37.0	37.0	33.0	37.0
37	35.24351001238646	37.0	37.0	37.0	33.0	37.0
38	35.21048720066061	37.0	37.0	37.0	33.0	37.0
39	35.09911488439306	37.0	37.0	37.0	33.0	37.0
40	34.87775340627581	37.0	37.0	37.0	27.0	37.0
41	35.03199060693642	37.0	37.0	37.0	27.0	37.0
42	35.04676661849711	37.0	37.0	37.0	27.0	37.0
43	34.69375	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	9.0
16	18.0
17	13.0
18	18.0
19	7.0
20	23.0
21	78.0
22	218.0
23	490.0
24	980.0
25	1775.0
26	2686.0
27	4248.0
28	5808.0
29	7918.0
30	10423.0
31	12890.0
32	16422.0
33	21209.0
34	30533.0
35	61674.0
36	210075.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91995251857969	21.420571841453345	14.563893476465731	23.095582163501238
2	15.400495458298927	23.085260115606935	39.63666391412056	21.877580511973576
3	17.63599298100743	28.14925681255161	31.458247316267546	22.75650289017341
4	12.002477291494634	18.32344137076796	40.663449628406276	29.01063170933113
5	13.167062345169281	37.81791907514451	35.310951692815856	13.704066886870356
6	27.19833815028902	41.531791907514446	17.744374483897605	13.525495458298927
7	24.493445499587118	33.72238852188274	23.985858794384807	17.798307184145333
8	24.09630470685384	35.34553055326177	21.54237200660611	19.015792733278282
9	25.715317919075144	15.165410817506192	21.078654004954583	38.040617258464074
10	14.30971304706854	28.625619322873657	34.3355181668043	22.72914946325351
11	32.2489161849711	24.905553261767135	24.835105284888524	18.010425268373247
12	22.0440235342692	26.762231626754744	31.51605078447564	19.677694054500414
13	28.02461808422791	23.25505780346821	27.037314203137903	21.68300990916598
14	21.673720066061104	22.28426919900908	29.62298720066061	26.419023534269197
15	23.183577621800165	29.389192815854663	26.159424029727496	21.267805532617672
16	21.33154417836499	29.56389347646573	26.385734929810074	22.718827415359208
17	21.208453757225435	28.087324525185796	27.787210982658962	22.91701073492981
18	22.03008876961189	26.88867671345995	29.67821015689513	21.40302436003303
19	23.70664739884393	26.66649463253509	30.106833195706027	19.520024772914944
20	23.62561932287366	26.248193641618496	29.752528901734106	20.373658133773738
21	22.09098885218827	27.65715317919075	29.337582576383152	20.91427539223782
22	21.366381090008257	27.69199009083402	29.634857555739057	21.30677126341866
23	21.37025185796862	27.113181255161024	29.976517341040466	21.54004954582989
24	21.70804087530966	27.31420313790256	29.572667217175884	21.40508876961189
25	21.841969446738233	27.22465937241949	30.020386044591245	20.912985136251034
26	21.811261354252682	27.259496284062756	29.499638728323703	21.42960363336086
27	21.734104046242773	27.360136251032202	29.437964492155245	21.467795210569776
28	21.074783236994218	27.700247729149464	29.86065235342692	21.364316680429397
29	22.080408753096613	27.526063170933114	29.0351465730801	21.358381502890172
30	21.801713459950452	27.770953757225435	29.540410817506192	20.88692196531792
31	21.981575144508668	27.205047481420312	29.540152766308836	21.27322460776218
32	20.823441370767963	27.293042939719243	29.834847233691164	22.048668455821634
33	20.97569157720892	27.207886044591245	30.548616845582167	21.267805532617672
34	21.18368084227911	27.109052436003306	29.59485962014864	22.11240710156895
35	21.342640379851364	27.853014037985137	29.52492774566474	21.279417836498762
36	21.813067712634187	27.574318744838976	29.302487613542528	21.31012592898431
37	22.06802229562345	27.233175061932286	29.10972336911643	21.589079273327826
38	21.80532617671346	27.506967382328657	29.279521056977703	21.408185383980182
39	21.554500412881914	27.269818331957058	29.36622625928984	21.80945499587118
40	21.400959950454173	27.624380677126343	29.577312138728324	21.397347233691164
41	21.575918662262595	27.18879025598679	29.680532617671346	21.554758464079274
42	21.27245045417011	27.688377374071017	29.30739058629232	21.731781585466557
43	21.140328241123036	27.574060693641616	29.455770024772914	21.829841040462426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	80.5
2	96.0
3	269.0
4	442.0
5	442.0
6	549.5
7	657.0
8	593.5
9	530.0
10	763.0
11	996.0
12	996.0
13	1549.0
14	2102.0
15	3734.0
16	5366.0
17	6050.5
18	6735.0
19	6735.0
20	6317.0
21	5899.0
22	6416.5
23	6934.0
24	8026.0
25	9118.0
26	9118.0
27	10449.0
28	11780.0
29	13958.5
30	16137.0
31	16981.0
32	17825.0
33	17825.0
34	19305.5
35	20786.0
36	21802.0
37	22818.0
38	23578.0
39	24338.0
40	24338.0
41	25307.0
42	26276.0
43	26104.0
44	25932.0
45	26172.0
46	26412.0
47	26412.0
48	26182.0
49	25952.0
50	26163.0
51	26374.0
52	26326.5
53	26279.0
54	26279.0
55	24008.5
56	21738.0
57	19286.0
58	16834.0
59	15025.0
60	13216.0
61	13216.0
62	11357.5
63	9499.0
64	7635.0
65	5771.0
66	4892.0
67	4013.0
68	4013.0
69	3253.0
70	2493.0
71	2172.5
72	1852.0
73	1441.0
74	1030.0
75	1030.0
76	748.0
77	466.0
78	358.5
79	251.0
80	215.0
81	179.0
82	179.0
83	151.5
84	124.0
85	115.0
86	106.0
87	84.0
88	62.0
89	62.0
90	42.5
91	23.0
92	17.0
93	11.0
94	7.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	387520.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.43338799244391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.26126032724656	57.51405419360018
2	6.072263406039322	7.825130080673012
3	1.5429800366814164	2.982582941042672
4	0.7068007919083968	1.821662786336014
5	0.43058375857487924	1.3871985189749998
6	0.30652400650786277	1.1850228144191715
7	0.21158224609935616	0.9543072668662799
8	0.1613554710343693	0.8317343735888851
9	0.13592108502167174	0.78820704067998
>10	1.0216148100518636	12.87694928670512
>50	0.09481709303085656	4.145244878294899
>100	0.052270961542162056	5.345713225576152
>500	8.104025045296443E-4	0.34593748590419543
>1k	0.0012156037567944665	1.9962551073384742
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3547	0.9153075970272502	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2671	0.6892547481420314	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.36849710982658956	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	757	0.1953447563996697	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	568	0.14657308009909165	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	489	0.12618703550784477	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	433	0.11173616845582164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.580511973575557E-4	0.0	2.580511973575557E-4	0.0	0.0
6	2.580511973575557E-4	0.0	0.001032204789430223	0.0	0.0
7	2.580511973575557E-4	0.0	0.004386870355078448	2.580511973575557E-4	0.0
8	2.580511973575557E-4	0.0	0.004644921552436003	2.580511973575557E-4	0.0
9	2.580511973575557E-4	0.0	0.005935177539223782	0.001032204789430223	0.0
10	7.741535920726672E-4	0.0	0.006967382328654005	0.001032204789430223	0.0
11	7.741535920726672E-4	0.0	0.01083815028901734	0.001032204789430223	0.0
12	7.741535920726672E-4	0.0	0.01212840627580512	0.0012902559867877788	0.0
13	7.741535920726672E-4	0.0	0.012644508670520232	0.0012902559867877788	0.0
14	7.741535920726672E-4	0.0	0.013418662262592898	0.0015483071841453345	0.0
15	7.741535920726672E-4	0.0	0.014450867052023121	0.0018063583815028901	0.0
16	7.741535920726672E-4	0.0	0.01625722543352601	0.0018063583815028901	0.0
17	7.741535920726672E-4	0.0	0.01703137902559868	0.002064409578860446	0.0
18	7.741535920726672E-4	0.0	0.01703137902559868	0.0023224607762180015	0.0
19	7.741535920726672E-4	0.0	0.017805532617671347	0.0023224607762180015	0.0
20	7.741535920726672E-4	0.0	0.018321635012386456	0.0025805119735755576	0.0
21	7.741535920726672E-4	0.0	0.018579686209744012	0.0036127167630057803	0.0
22	7.741535920726672E-4	0.0	0.018837737407101568	0.003870767960363336	0.0
23	7.741535920726672E-4	2.580511973575557E-4	0.019611890999174236	0.004386870355078448	0.0
24	7.741535920726672E-4	2.580511973575557E-4	0.019611890999174236	0.00541907514450867	0.0
25	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.0056771263418662265	0.0
26	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.006193228736581338	0.0
27	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.008773740710156895	0.0
28	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.022192402972749794	0.0
29	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.05083608587943848	0.0
30	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.08360858794384805	0.0
31	7.741535920726672E-4	2.580511973575557E-4	0.019869942196531792	0.1486374896779521	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGA	25	1.2321162E-4	36.999996	32
GGACCGT	40	5.9301266E-5	27.75	6
GGTATCA	410	0.0	26.62195	1
TCTTTCG	35	8.858746E-4	26.42857	10
TGGACCG	50	9.0708E-6	25.899998	5
CTGCTCG	95	1.0913936E-11	25.31579	9
CGAGTCG	45	1.3210022E-4	24.666666	21
ATAAGAC	45	1.3210022E-4	24.666666	3
ATTATAC	45	1.3210022E-4	24.666666	3
GTATCAA	1265	0.0	23.98419	1
TCACGGT	40	0.0019288901	23.125	1
TTTCGAG	40	0.0019288901	23.125	12
GCCGGCA	80	2.7139322E-8	23.125	15
GCTTCGC	80	2.7139322E-8	23.125	22
TGCTCGC	80	2.7139322E-8	23.125	10
TTCGCCG	80	2.7139322E-8	23.125	24
GCCGCTC	75	3.7275458E-7	22.2	27
AGCTTCG	85	5.158472E-8	21.764706	21
ACTGCTC	145	0.0	21.689657	8
CCGCTCT	70	5.087535E-6	21.142857	28
>>END_MODULE