FastQCFastQC Report
Fri 10 Feb 2017
ERR1633513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544799
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38970.7153096830207104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28890.5302873169737831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15480.2841414907149242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.14721025552543232No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6130.11251856189163344No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5870.10774615959280395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.024.763781
GCCCCGA551.9002775E-523.54545411
GTATCAA16200.023.524691
TAGACAG400.001929889823.1250025
TTCTGCG502.6995747E-422.19999918
GTCAACC603.7216552E-521.58333415
TTAGACT450.00382326420.5555554
CGAATTA555.1383214E-420.18181815
GCCGGCA609.228314E-418.515
CCGTACA1103.8406142E-818.517
CGAGGGT1501.2732926E-1118.516
ATAGACC500.007030248618.4999988
ACCATGC1351.1477823E-917.8148168
ATAACAC852.7188597E-517.4117643
TGCGGGT752.0647523E-417.26666621
TCCACGA1502.5102054E-1017.26666612
TGGGGCG650.001578492317.07692337
CCGAATT650.001578492317.07692314
CGTCAAT650.001578492317.07692330
CCCGAAT650.001578492317.07692313
Produced by FastQC (version 0.11.5)