##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544799
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87174535929765	33.0	33.0	33.0	33.0	33.0
2	31.999691629389922	33.0	33.0	33.0	33.0	33.0
3	31.715406966606032	33.0	33.0	33.0	27.0	33.0
4	31.989333680862117	33.0	33.0	33.0	33.0	33.0
5	32.085306691091574	33.0	33.0	33.0	33.0	33.0
6	35.26781436823489	37.0	37.0	37.0	33.0	37.0
7	35.40417107960918	37.0	37.0	37.0	33.0	37.0
8	35.53325538409579	37.0	37.0	37.0	33.0	37.0
9	35.65354011295909	37.0	37.0	37.0	33.0	37.0
10	35.651625645421525	37.0	37.0	37.0	33.0	37.0
11	35.65833821280876	37.0	37.0	37.0	33.0	37.0
12	35.658633734643416	37.0	37.0	37.0	33.0	37.0
13	35.66405958894932	37.0	37.0	37.0	33.0	37.0
14	35.63916600434288	37.0	37.0	37.0	33.0	37.0
15	35.65668072077959	37.0	37.0	37.0	33.0	37.0
16	35.6371505821413	37.0	37.0	37.0	33.0	37.0
17	35.63047472554098	37.0	37.0	37.0	33.0	37.0
18	35.6183307972298	37.0	37.0	37.0	33.0	37.0
19	35.62514248374171	37.0	37.0	37.0	33.0	37.0
20	35.6228168553907	37.0	37.0	37.0	33.0	37.0
21	35.61057564349421	37.0	37.0	37.0	33.0	37.0
22	35.5256452379685	37.0	37.0	37.0	33.0	37.0
23	35.56355646761466	37.0	37.0	37.0	33.0	37.0
24	35.5500597468057	37.0	37.0	37.0	33.0	37.0
25	35.57495149587279	37.0	37.0	37.0	33.0	37.0
26	35.470602919608886	37.0	37.0	37.0	33.0	37.0
27	35.47804786719506	37.0	37.0	37.0	33.0	37.0
28	35.50335628369362	37.0	37.0	37.0	33.0	37.0
29	35.49797631787136	37.0	37.0	37.0	33.0	37.0
30	35.49325714621356	37.0	37.0	37.0	33.0	37.0
31	35.49135185637272	37.0	37.0	37.0	33.0	37.0
32	35.4783709221199	37.0	37.0	37.0	33.0	37.0
33	35.46911429719952	37.0	37.0	37.0	33.0	37.0
34	35.4286388190874	37.0	37.0	37.0	33.0	37.0
35	35.37902235503369	37.0	37.0	37.0	33.0	37.0
36	35.39487957944122	37.0	37.0	37.0	33.0	37.0
37	35.395657848123804	37.0	37.0	37.0	33.0	37.0
38	35.37589276044927	37.0	37.0	37.0	33.0	37.0
39	35.265422660467436	37.0	37.0	37.0	33.0	37.0
40	35.04436865706435	37.0	37.0	37.0	27.0	37.0
41	35.21525737014936	37.0	37.0	37.0	33.0	37.0
42	35.24894318822171	37.0	37.0	37.0	33.0	37.0
43	34.90880673422675	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	13.0
16	13.0
17	13.0
18	18.0
19	22.0
20	24.0
21	86.0
22	252.0
23	608.0
24	1190.0
25	2132.0
26	3628.0
27	5480.0
28	7493.0
29	10086.0
30	13211.0
31	17055.0
32	21681.0
33	28429.0
34	41089.0
35	83301.0
36	308975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49052953474584	21.71626599901982	14.564270492420139	23.228933973814193
2	15.226533088350017	22.715533618820885	40.13627044102504	21.92166285180406
3	17.362366671010776	28.364956617027566	31.391760998092877	22.880915713868784
4	11.949728248399868	18.461120523348978	41.253012578951136	28.33613864930002
5	12.74121281426728	38.173344664729555	35.95674735085784	13.128695170145319
6	27.20140822578602	41.533115883105516	18.120995082590095	13.144480808518372
7	24.58576465815833	33.6981161859695	24.315940374339895	17.400178781532272
8	23.715902562229374	35.97161521955804	21.612925133856706	18.69955708435588
9	25.39266775452965	15.697899592326712	21.160097577271618	37.74933507587202
10	14.137507594544044	28.880743173170288	34.831745285876075	22.150003946409594
11	32.09936141586163	24.947182355327378	25.214803991930967	17.73865223688002
12	21.757014972494442	26.92295690704278	31.55952929429019	19.760498826172586
13	27.272076490595616	23.42368469839335	27.510145943733377	21.794092867277655
14	21.355582517589056	22.404409699724116	29.38404806176223	26.8559597209246
15	22.87485843402796	29.60229368996639	26.28327144506506	21.239576430940584
16	21.43359293978146	29.9187406731657	26.405334811554354	22.242331575498486
17	21.067219286379014	28.418370811987543	27.879456460088953	22.634953441544496
18	21.67900455030204	27.83356797644636	29.518225987933167	20.96920148531844
19	23.442957861523244	27.10797927308971	29.804937233732076	19.644125631654976
20	23.510322155510565	26.690210518007557	29.56760199633259	20.231865330149283
21	21.674599255872348	28.009229091830196	29.1079829441684	21.208188708129054
22	21.156059390711068	27.893406559116297	29.31411401269092	21.636420037481713
23	21.226911209455228	27.679015563538112	29.80310169438637	21.290971532620286
24	21.717183768692674	27.82328895611042	28.9921604114545	21.467366863742406
25	21.885502726693698	27.486283932239232	29.65185325230039	20.976360088766683
26	21.731868083458302	27.790616355756892	29.135883142223097	21.34163241856171
27	21.579701871699474	27.95416291145909	28.885148467599976	21.58098674924146
28	21.24618437258512	27.861101066631917	29.521897066624568	21.370817494158395
29	22.06813889159121	27.851556262034254	28.778320077680025	21.30198476869451
30	21.58979733810084	27.9664610250753	29.537866258932194	20.905875377891665
31	21.880913878329437	27.500601139135718	29.31870286105518	21.299782121479666
32	20.66248286065136	27.60100514134571	29.726376149735955	22.010135848266977
33	21.009032689120207	27.49491096716404	30.152771939742912	21.34328440397284
34	21.32364413297381	27.45654819483883	29.392675096687036	21.827132575500322
35	21.409180266483602	28.334853771758024	29.05732205822698	21.198643903531394
36	21.90165547293589	27.738119930469768	29.093482183337343	21.266742413257
37	21.994166655959354	27.24527761614834	28.891022193506227	21.869533534386076
38	21.85613409716244	27.824573833652412	28.918004621888073	21.401287447297076
39	21.57437880759693	27.586504380514647	29.186176920295377	21.652939891593046
40	21.410098036156455	28.118810790768705	29.250053689525863	21.22103748354898
41	21.254994961444496	27.580630654608395	29.482616524626515	21.681757859320594
42	21.40147100123165	27.847885183342846	29.248768811983872	21.501875003441636
43	21.422763257641808	27.35559353082513	29.29025200119677	21.93139121033629
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	82.0
2	84.0
3	297.5
4	511.0
5	511.0
6	599.0
7	687.0
8	632.5
9	578.0
10	819.0
11	1060.0
12	1060.0
13	1634.0
14	2208.0
15	4117.5
16	6027.0
17	6893.0
18	7759.0
19	7759.0
20	7745.0
21	7731.0
22	8694.5
23	9658.0
24	11467.0
25	13276.0
26	13276.0
27	15398.0
28	17520.0
29	20756.0
30	23992.0
31	25677.0
32	27362.0
33	27362.0
34	29574.0
35	31786.0
36	33366.5
37	34947.0
38	36213.5
39	37480.0
40	37480.0
41	38434.5
42	39389.0
43	38388.5
44	37388.0
45	37300.0
46	37212.0
47	37212.0
48	36060.5
49	34909.0
50	35276.0
51	35643.0
52	35259.5
53	34876.0
54	34876.0
55	31874.0
56	28872.0
57	25630.5
58	22389.0
59	19608.5
60	16828.0
61	16828.0
62	14679.5
63	12531.0
64	10124.5
65	7718.0
66	6447.5
67	5177.0
68	5177.0
69	4280.5
70	3384.0
71	2913.5
72	2443.0
73	1956.0
74	1469.0
75	1469.0
76	1082.0
77	695.0
78	541.0
79	387.0
80	310.5
81	234.0
82	234.0
83	216.5
84	199.0
85	171.0
86	143.0
87	117.0
88	91.0
89	91.0
90	75.0
91	59.0
92	37.0
93	15.0
94	8.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	544799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.80534668584268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67660435312474	53.92011670615056
2	6.524981982719148	7.935075831562298
3	1.5805918735242415	2.883253105154013
4	0.7240008972207304	1.7609250222547064
5	0.4293517522338928	1.305344107237794
6	0.30177088605998575	1.1009570007942817
7	0.20712122035377944	0.8815854326724462
8	0.16698627386643783	0.8122926619380658
9	0.12986681590126628	0.7106937087476703
>10	1.043687824033487	13.248758799665088
>50	0.14040054074694752	5.858238552872027
>100	0.07280027906255546	7.660329860956285
>500	9.176505764187649E-4	0.3723590633136131
>1k	9.176505764187649E-4	1.5500701466811448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3897	0.7153096830207104	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2889	0.5302873169737831	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1548	0.2841414907149242	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	802	0.14721025552543232	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	613	0.11251856189163344	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	587	0.10774615959280395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.835539345703645E-4	0.0	0.0
5	0.0	0.0	3.67107869140729E-4	0.0	0.0
6	0.0	0.0	5.506618037110935E-4	0.0	0.0
7	0.0	0.0	0.0016519854111332803	0.0	0.0
8	0.0	0.0	0.002386201149414738	0.0	0.0
9	0.0	0.0	0.003120416887696196	7.34215738281458E-4	0.0
10	0.0	0.0	0.0033039708222665607	9.177696728518225E-4	0.0
11	0.0	0.0	0.006424387709962757	0.0012848775419925513	0.0
12	0.0	0.0	0.0067914955791034855	0.0012848775419925513	0.0
13	0.0	0.0	0.0067914955791034855	0.0012848775419925513	0.0
14	0.0	0.0	0.007158603448244215	0.0012848775419925513	0.0
15	0.0	0.0	0.007892819186525672	0.0012848775419925513	0.0
16	0.0	0.0	0.008810588859377496	0.0016519854111332803	0.0
17	0.0	0.0	0.008994142793947859	0.0016519854111332803	0.0
18	0.0	0.0	0.009177696728518224	0.0016519854111332803	0.0
19	0.0	0.0	0.009177696728518224	0.0016519854111332803	0.0
20	0.0	0.0	0.009544804597658952	0.001835539345703645	0.0
21	0.0	0.0	0.009911912466799682	0.0020190932802740093	0.0
22	0.0	0.0	0.010095466401370047	0.0025697550839851027	0.0
23	0.0	0.0	0.01027902033594041	0.0033039708222665607	0.0
24	0.0	0.0	0.01027902033594041	0.004038186560548019	0.0
25	0.0	0.0	0.01027902033594041	0.004221740495118383	0.0
26	0.0	0.0	0.01027902033594041	0.004955956233399841	0.0
27	0.0	0.0	0.01027902033594041	0.006607941644533121	0.0
28	0.0	0.0	0.01027902033594041	0.01688696198047353	0.0
29	0.0	0.0	0.01027902033594041	0.036160125110361804	0.0
30	0.0	0.0	0.01027902033594041	0.05983858266993882	0.0
31	0.0	0.0	0.01027902033594041	0.11545542484475926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	635	0.0	24.76378	1
GCCCCGA	55	1.9002775E-5	23.545454	11
GTATCAA	1620	0.0	23.52469	1
TAGACAG	40	0.0019298898	23.125002	5
TTCTGCG	50	2.6995747E-4	22.199999	18
GTCAACC	60	3.7216552E-5	21.583334	15
TTAGACT	45	0.003823264	20.555555	4
CGAATTA	55	5.1383214E-4	20.181818	15
GCCGGCA	60	9.228314E-4	18.5	15
CCGTACA	110	3.8406142E-8	18.5	17
CGAGGGT	150	1.2732926E-11	18.5	16
ATAGACC	50	0.0070302486	18.499998	8
ACCATGC	135	1.1477823E-9	17.814816	8
ATAACAC	85	2.7188597E-5	17.411764	3
TGCGGGT	75	2.0647523E-4	17.266666	21
TCCACGA	150	2.5102054E-10	17.266666	12
TGGGGCG	65	0.0015784923	17.076923	37
CCGAATT	65	0.0015784923	17.076923	14
CGTCAAT	65	0.0015784923	17.076923	30
CCCGAAT	65	0.0015784923	17.076923	13
>>END_MODULE