FastQCFastQC Report
Fri 10 Feb 2017
ERR1633512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341742
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19620.5741173165721509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.45736257176466455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8190.23965447618378774No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5810.1700112950705503No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5550.16240321646154116No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5130.15011324332391102No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4770.13957898063451374No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4440.12992257316923295No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4420.1293373363531553No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4370.12787424431296124No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4280.12524067864061192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.12143663933610735No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3760.11002452142259365No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3760.11002452142259365No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3640.1065131005261279No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3460.10124596918142928No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3420.10007549554927403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATC303.594233E-430.8333347
GGTATCA4150.024.0722891
TTAACGG1300.022.7692335
TAGGCTG502.6967213E-422.1999995
ATAATAT1350.021.9259263
TAACGGC1400.021.14285936
GACACAT1600.020.812526
CACGATA450.00382039120.55555516
GTCTTGA450.00382039120.55555511
CTCTTAC1450.020.41379431
CACGAGG1550.020.29032114
GTGACAC1650.020.18181824
GGCAGTC656.884004E-519.92307712
AACGGCC1403.6379788E-1219.8214337
CGAGGGT1500.019.73333416
TCCACGA1500.019.73333412
CTAGCAT951.6675222E-719.4736844
CCACGAG1551.8189894E-1219.09677313
CACATGT1650.019.06060628
GTATCAA7950.018.8490561
Produced by FastQC (version 0.11.5)