##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633512.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341742
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312861749506936	33.0	33.0	33.0	27.0	33.0
2	31.568800440098087	33.0	33.0	33.0	27.0	33.0
3	31.294889126885195	33.0	33.0	33.0	27.0	33.0
4	31.604315536281756	33.0	33.0	33.0	27.0	33.0
5	31.72422178134382	33.0	33.0	33.0	27.0	33.0
6	34.57876994925997	37.0	37.0	37.0	27.0	37.0
7	34.75347191741138	37.0	37.0	37.0	27.0	37.0
8	34.886273855715714	37.0	37.0	37.0	27.0	37.0
9	35.02095440419966	37.0	37.0	37.0	27.0	37.0
10	35.048735595858865	37.0	37.0	37.0	27.0	37.0
11	35.039684908498224	37.0	37.0	37.0	27.0	37.0
12	35.03513176606914	37.0	37.0	37.0	27.0	37.0
13	35.051023871809726	37.0	37.0	37.0	33.0	37.0
14	35.024743812583765	37.0	37.0	37.0	27.0	37.0
15	35.01621398598943	37.0	37.0	37.0	27.0	37.0
16	35.00874929040036	37.0	37.0	37.0	27.0	37.0
17	35.00801481819618	37.0	37.0	37.0	27.0	37.0
18	34.99647687436721	37.0	37.0	37.0	27.0	37.0
19	34.975405422804336	37.0	37.0	37.0	27.0	37.0
20	34.96974910897695	37.0	37.0	37.0	27.0	37.0
21	34.985181803816914	37.0	37.0	37.0	27.0	37.0
22	34.8775626057084	37.0	37.0	37.0	27.0	37.0
23	34.91204183272761	37.0	37.0	37.0	27.0	37.0
24	34.910119329786795	37.0	37.0	37.0	27.0	37.0
25	34.92129150060572	37.0	37.0	37.0	27.0	37.0
26	34.80658508465451	37.0	37.0	37.0	27.0	37.0
27	34.794912536357835	37.0	37.0	37.0	27.0	37.0
28	34.819176454752416	37.0	37.0	37.0	27.0	37.0
29	34.815764524114684	37.0	37.0	37.0	27.0	37.0
30	34.82298634642508	37.0	37.0	37.0	27.0	37.0
31	34.80452797724599	37.0	37.0	37.0	27.0	37.0
32	34.79825716476172	37.0	37.0	37.0	27.0	37.0
33	34.800027506130355	37.0	37.0	37.0	27.0	37.0
34	34.759028740980035	37.0	37.0	37.0	27.0	37.0
35	34.69622112587859	37.0	37.0	37.0	27.0	37.0
36	34.72679389715048	37.0	37.0	37.0	27.0	37.0
37	34.726758782941516	37.0	37.0	37.0	27.0	37.0
38	34.71140509507172	37.0	37.0	37.0	27.0	37.0
39	34.59844268483242	37.0	37.0	37.0	27.0	37.0
40	34.3760409899866	37.0	37.0	37.0	27.0	37.0
41	34.58378542877375	37.0	37.0	37.0	27.0	37.0
42	34.59195240854211	37.0	37.0	37.0	27.0	37.0
43	34.17462296118124	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	19.0
17	23.0
18	20.0
19	21.0
20	54.0
21	159.0
22	396.0
23	840.0
24	1469.0
25	2542.0
26	3857.0
27	5438.0
28	7129.0
29	9297.0
30	11658.0
31	14723.0
32	18380.0
33	23555.0
34	33971.0
35	62725.0
36	145456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18148193666567	21.929701353652757	13.78437534748436	24.104441362197214
2	16.18794295111517	22.239876866173898	38.161829684381786	23.410350498329148
3	17.424255725079156	28.2689865454056	30.225725839961143	24.081031889554108
4	12.393852672483922	18.847551661779942	40.23795728941717	28.52063837631898
5	13.4733219797391	37.8724300788314	35.53792041949775	13.116327521931751
6	28.157791550350847	41.40579735590006	17.523160746996272	12.913250346752815
7	25.916334544773544	32.73053941277338	23.961643579074273	17.391482463378807
8	24.05615932487081	36.18928899579215	20.771225076227097	18.983326603109948
9	25.60820736110867	15.240444545885493	20.67670933043056	38.474638762575275
10	14.93758449356532	28.40710243399992	34.03737322307472	22.617939849360045
11	32.98950670388773	24.32507564185848	24.62062023397768	18.064797420276115
12	22.52722814286801	26.66046315641624	30.080001872757812	20.732306827957935
13	27.04935302069983	23.023801581309876	26.517372754885265	23.409472643105033
14	21.37957874653979	21.59523851326439	28.615446740523552	28.40973599967227
15	23.589140345640864	29.102949008316216	25.390499265527794	21.917411380515126
16	22.24602185274271	28.95371362021642	25.768269630305902	23.031994896734965
17	21.696484482445822	28.20402525882098	26.888705514686517	23.210784744046677
18	21.939650379526075	27.894434983115918	28.868561663477127	21.29735297388088
19	24.195738305505323	26.495718992690392	28.324876661341015	20.983666040463273
20	23.234194216689783	26.776047427591575	28.550778072346976	21.438980283371667
21	22.250411128863295	27.81045350000878	28.507177929549194	21.431957441578735
22	22.05406417706925	27.412492465075992	28.230068297136434	22.30337506071832
23	21.99202907456502	27.386742045168578	29.040328668995908	21.58090021127049
24	22.362191360734123	27.92398944232784	27.24774830135014	22.4660708955879
25	22.321224783608688	27.547682169589926	28.61837292460394	21.51272012219745
26	22.57931421949892	27.7419807925277	28.467674444463952	21.21103054350943
27	22.42188551597404	27.63488245518549	27.73876199003927	22.2044700388012
28	21.818213740189968	27.238091893884857	29.11875040235031	21.82494396357486
29	23.539395216274265	27.16405943665104	27.825084420410718	21.471460926663973
30	22.775368552884924	26.773413861919227	28.655242844016833	21.79597474117902
31	22.272357509466207	27.24306640682152	28.338044489702757	22.146531594009517
32	21.278040158950322	27.091782689865457	28.807404416197013	22.82277273498721
33	21.38367540425233	27.184542725213756	29.31100069643181	22.1207811741021
34	22.29137770598873	27.042622797314937	28.843981717201867	21.822017779494473
35	22.29079246917265	27.781191659204897	28.14579419562126	21.782221676001193
36	23.213710928127064	27.36362519093351	27.65214694125978	21.77051693967964
37	22.390575346313884	26.98702529978756	27.976075518958748	22.646323834939807
38	22.375944425911946	27.344604994410986	28.060057002065886	22.21939357761118
39	21.6918025879172	27.445558345184377	28.464748260383566	22.397890806514855
40	21.956037010376246	28.058593910025692	28.563360663892645	21.422008415705417
41	22.014560691984013	26.790970966401556	28.32751022701336	22.866958114601076
42	21.47819115004887	28.087855750829572	28.14579419562126	22.288158903500303
43	22.516401261770575	26.578237383757337	28.247625401618766	22.657735952853322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	37.5
2	36.0
3	118.5
4	201.0
5	201.0
6	246.5
7	292.0
8	274.0
9	256.0
10	367.0
11	478.0
12	478.0
13	786.5
14	1095.0
15	1989.0
16	2883.0
17	3242.5
18	3602.0
19	3602.0
20	3493.0
21	3384.0
22	3659.0
23	3934.0
24	4852.5
25	5771.0
26	5771.0
27	6965.0
28	8159.0
29	9340.5
30	10522.0
31	12213.0
32	13904.0
33	13904.0
34	16839.0
35	19774.0
36	21358.5
37	22943.0
38	24119.5
39	25296.0
40	25296.0
41	26349.5
42	27403.0
43	27895.5
44	28388.0
45	27585.5
46	26783.0
47	26783.0
48	25249.0
49	23715.0
50	23464.5
51	23214.0
52	22349.5
53	21485.0
54	21485.0
55	20660.0
56	19835.0
57	16913.0
58	13991.0
59	12532.0
60	11073.0
61	11073.0
62	9750.5
63	8428.0
64	6689.0
65	4950.0
66	4104.5
67	3259.0
68	3259.0
69	2603.0
70	1947.0
71	1761.5
72	1576.0
73	1479.5
74	1383.0
75	1383.0
76	1164.0
77	945.0
78	695.0
79	445.0
80	294.5
81	144.0
82	144.0
83	110.0
84	76.0
85	70.5
86	65.0
87	57.0
88	49.0
89	49.0
90	33.5
91	18.0
92	9.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	341742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.24724264843929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.62975728760237	49.28932221379612
2	6.287290134132066	7.072854675513296
3	1.777149113699271	2.9987921226210514
4	0.9509168979006392	2.1394581397887373
5	0.5671235867308643	1.5949568997252064
6	0.3704495026767983	1.2502057839633323
7	0.31311899987355946	1.2328456254607312
8	0.2372048888476629	1.0673696752328452
9	0.2074572816995509	1.0502010056646238
>10	1.4070825479333824	15.59311533955903
>50	0.17147530793467483	6.817893165049433
>100	0.07779898230369506	8.10895558730064
>500	0.002116979110304627	0.7346880466027159
>1k	0.0010584895551523136	1.0493417197222745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1962	0.5741173165721509	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1563	0.45736257176466455	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	819	0.23965447618378774	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	581	0.1700112950705503	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	555	0.16240321646154116	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	513	0.15011324332391102	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	477	0.13957898063451374	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	444	0.12992257316923295	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	442	0.1293373363531553	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	437	0.12787424431296124	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	428	0.12524067864061192	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.12143663933610735	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	376	0.11002452142259365	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	376	0.11002452142259365	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	364	0.1065131005261279	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	346	0.10124596918142928	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	342	0.10007549554927403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.926184080388129E-4	0.0
5	0.0	0.0	0.0	2.926184080388129E-4	0.0
6	0.0	0.0	5.852368160776258E-4	2.926184080388129E-4	0.0
7	0.0	0.0	0.0017557104482328774	2.926184080388129E-4	0.0
8	0.0	0.0	0.002340947264310503	2.926184080388129E-4	0.0
9	0.0	0.0	0.002633565672349316	0.0011704736321552515	0.0
10	0.0	0.0	0.002633565672349316	0.0011704736321552515	0.0
11	0.0	0.0	0.00409665771254338	0.0014630920401940645	0.0
12	0.0	0.0	0.004389276120582194	0.0014630920401940645	0.0
13	0.0	0.0	0.004681894528621006	0.0014630920401940645	0.0
14	0.0	0.0	0.0049745129366598195	0.0017557104482328774	0.0
15	0.0	0.0	0.005559749752737445	0.00204832885627169	0.0
16	0.0	0.0	0.006437604976853884	0.00204832885627169	2.926184080388129E-4
17	0.0	0.0	0.006437604976853884	0.002633565672349316	2.926184080388129E-4
18	0.0	0.0	0.007315460200970323	0.002633565672349316	2.926184080388129E-4
19	0.0	0.0	0.007315460200970323	0.002926184080388129	2.926184080388129E-4
20	0.0	0.0	0.007315460200970323	0.002926184080388129	2.926184080388129E-4
21	0.0	0.0	0.007900697017047948	0.003511420896465755	2.926184080388129E-4
22	0.0	0.0	0.007900697017047948	0.00409665771254338	2.926184080388129E-4
23	0.0	0.0	0.007900697017047948	0.00409665771254338	2.926184080388129E-4
24	0.0	0.0	0.007900697017047948	0.00409665771254338	2.926184080388129E-4
25	0.0	0.0	0.007900697017047948	0.004389276120582194	2.926184080388129E-4
26	0.0	0.0	0.007900697017047948	0.0049745129366598195	2.926184080388129E-4
27	0.0	0.0	0.007900697017047948	0.00702284179293151	2.926184080388129E-4
28	0.0	0.0	0.007900697017047948	0.017264486074289962	2.926184080388129E-4
29	0.0	0.0	0.007900697017047948	0.04125919553347262	2.926184080388129E-4
30	0.0	0.0	0.007900697017047948	0.07169150996950917	2.926184080388129E-4
31	0.0	0.0	0.007900697017047948	0.12641115227276717	2.926184080388129E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTATC	30	3.594233E-4	30.833334	7
GGTATCA	415	0.0	24.072289	1
TTAACGG	130	0.0	22.76923	35
TAGGCTG	50	2.6967213E-4	22.199999	5
ATAATAT	135	0.0	21.925926	3
TAACGGC	140	0.0	21.142859	36
GACACAT	160	0.0	20.8125	26
CACGATA	45	0.003820391	20.555555	16
GTCTTGA	45	0.003820391	20.555555	11
CTCTTAC	145	0.0	20.413794	31
CACGAGG	155	0.0	20.290321	14
GTGACAC	165	0.0	20.181818	24
GGCAGTC	65	6.884004E-5	19.923077	12
AACGGCC	140	3.6379788E-12	19.82143	37
CGAGGGT	150	0.0	19.733334	16
TCCACGA	150	0.0	19.733334	12
CTAGCAT	95	1.6675222E-7	19.473684	4
CCACGAG	155	1.8189894E-12	19.096773	13
CACATGT	165	0.0	19.060606	28
GTATCAA	795	0.0	18.849056	1
>>END_MODULE