FastQCFastQC Report
Fri 10 Feb 2017
ERR1633511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429046
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27650.6444530423311253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.5113670795206108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10910.254285088312209No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8650.20161008376724174No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8320.19391860080271114No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7870.18343021494198758No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7690.17923486059769816No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7260.16921262521967342No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6300.14683740205012982No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6280.146371251567431No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6200.1445066496366357No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6140.14310819818853923No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5960.1389128438442498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.1354167152240086No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5690.13261981232781567No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5640.1314544361210686No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5500.1281913827421768No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5390.12562755508733328No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5350.12469525412193563No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5290.12329680267383918No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5190.12096605026034504No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5040.11746992164010385No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5040.11746992164010385No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4970.11583839495065797No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4680.109079212951525No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4660.10861306246882618No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4620.10768076150342853No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4580.10674846053803089No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4410.10278618143509087No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4350.1013877299869944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAC200.001840650237.031
TACCCGA250.005492552629.59999830
ATTCGTG250.005492552629.59999811
GTCGGAA250.005492552629.59999821
ATTGTAC358.860299E-426.428573
CCGCTCT358.860299E-426.4285728
GGTATCA4950.023.1717171
TCACGGT400.00192922523.1250021
CGTGCAG400.00192922523.12500214
CTTAGGA400.00192922523.1250022
ATACCAT400.00192922523.1250026
TCTATAC652.6763428E-622.7692283
GTATCAA13200.022.7045461
AGGACCG502.698279E-422.1999995
TGCTCGC502.698279E-422.19999910
GGACCGT502.698279E-422.1999996
AGCTTCG705.0896324E-621.14285721
ATTGACG806.939208E-720.812517
TCGGAAC450.003821958820.55555522
ACGGTAC450.003821958820.5555553
Produced by FastQC (version 0.11.5)