##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633511.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429046
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841958671098205	33.0	33.0	33.0	27.0	33.0
2	31.953711257068008	33.0	33.0	33.0	33.0	33.0
3	31.665849349487	33.0	33.0	33.0	27.0	33.0
4	31.968117637735816	33.0	33.0	33.0	33.0	33.0
5	32.05995394433231	33.0	33.0	33.0	33.0	33.0
6	35.208089575476755	37.0	37.0	37.0	33.0	37.0
7	35.34979232995996	37.0	37.0	37.0	33.0	37.0
8	35.488691189289725	37.0	37.0	37.0	33.0	37.0
9	35.6081422504813	37.0	37.0	37.0	33.0	37.0
10	35.5826135192963	37.0	37.0	37.0	33.0	37.0
11	35.60510761083893	37.0	37.0	37.0	33.0	37.0
12	35.60586976687814	37.0	37.0	37.0	33.0	37.0
13	35.60144599879733	37.0	37.0	37.0	33.0	37.0
14	35.57752315602523	37.0	37.0	37.0	33.0	37.0
15	35.60241326104893	37.0	37.0	37.0	33.0	37.0
16	35.57074998951161	37.0	37.0	37.0	33.0	37.0
17	35.577826153838984	37.0	37.0	37.0	33.0	37.0
18	35.56695319383003	37.0	37.0	37.0	33.0	37.0
19	35.55949478610685	37.0	37.0	37.0	33.0	37.0
20	35.545894845774114	37.0	37.0	37.0	33.0	37.0
21	35.563091137080875	37.0	37.0	37.0	33.0	37.0
22	35.447334318464684	37.0	37.0	37.0	33.0	37.0
23	35.503570712697474	37.0	37.0	37.0	33.0	37.0
24	35.491443807890064	37.0	37.0	37.0	33.0	37.0
25	35.515399281195954	37.0	37.0	37.0	33.0	37.0
26	35.40410818420403	37.0	37.0	37.0	33.0	37.0
27	35.4165567328445	37.0	37.0	37.0	33.0	37.0
28	35.427066095476945	37.0	37.0	37.0	33.0	37.0
29	35.445751737575925	37.0	37.0	37.0	33.0	37.0
30	35.423355537634656	37.0	37.0	37.0	33.0	37.0
31	35.4062291689003	37.0	37.0	37.0	33.0	37.0
32	35.402346135379425	37.0	37.0	37.0	33.0	37.0
33	35.401693524703646	37.0	37.0	37.0	33.0	37.0
34	35.38207558163926	37.0	37.0	37.0	33.0	37.0
35	35.316178218652546	37.0	37.0	37.0	33.0	37.0
36	35.33956964987437	37.0	37.0	37.0	33.0	37.0
37	35.331980720016034	37.0	37.0	37.0	33.0	37.0
38	35.30795765489015	37.0	37.0	37.0	33.0	37.0
39	35.19510495378118	37.0	37.0	37.0	33.0	37.0
40	34.982976184371836	37.0	37.0	37.0	27.0	37.0
41	35.15136372323713	37.0	37.0	37.0	33.0	37.0
42	35.17553362576507	37.0	37.0	37.0	33.0	37.0
43	34.847349701430616	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	15.0
17	14.0
18	15.0
19	15.0
20	34.0
21	83.0
22	194.0
23	503.0
24	1081.0
25	1931.0
26	3020.0
27	4505.0
28	6192.0
29	8391.0
30	10897.0
31	13743.0
32	17521.0
33	22995.0
34	32995.0
35	66833.0
36	238059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.874871225929155	21.437328398353557	13.753769992028827	23.934030383688462
2	16.193601618474478	21.82959402954462	38.90841541466416	23.068388937316744
3	17.236613323513097	28.372482204705324	29.879779790511975	24.511124681269607
4	11.941143840054448	18.231145378351037	40.75460440139286	29.073106380201658
5	13.275266521538484	37.875192869762216	35.533252844683325	13.31628776401598
6	28.205134181416447	41.21073264871366	17.408855926870313	13.175277242999586
7	26.129366081958576	32.63729297091687	23.762487006055295	17.470853941069254
8	24.0776047323597	35.64676048722048	20.62972268707784	19.645912093341973
9	25.91120765605553	15.256639148249837	20.46377311523706	38.36838008045758
10	15.395785067335439	27.740615225407065	33.57495466686556	23.288645040391938
11	33.27475375600751	24.297161609710844	24.206029190343227	18.222055443938412
12	23.146469143168797	26.560555278454988	29.549745248761205	20.743230329615006
13	27.272833216018793	22.95861049864117	26.132162984854773	23.636393300485263
14	21.854766155610353	21.350391333330226	28.531439519305625	28.2634029917538
15	24.289470126746316	28.583415298126543	25.452748656321234	21.67436591880591
16	22.533248183178493	28.60882049943363	25.36837541895275	23.489555898435132
17	21.915132643119854	27.78210261836726	26.385515772201586	23.917248966311305
18	22.18130456874088	27.57886100791057	28.51582347813521	21.724010945213333
19	24.655864406147593	26.182274161744896	28.102581075222705	21.05928035688481
20	23.74570558867814	26.188101042778626	28.436577896076408	21.62961547246682
21	22.633936687441437	27.365830237317212	28.42795411214648	21.572278963094867
22	22.137486423367193	27.192655332994597	28.094423441775472	22.575434801862738
23	22.14308022915958	27.09639525831729	28.87359397360656	21.886930538916573
24	22.553758804417242	27.98114887447966	26.500888016669542	22.964204304433558
25	22.471949394703596	27.374687096488486	28.437044046559112	21.716319462248805
26	22.790097099145544	27.474210224544688	28.319574124919004	21.41611855139076
27	22.71784377432723	27.28728388098246	27.36280025917967	22.632072085510643
28	21.785775884170928	26.97356460612615	29.081030938407537	22.159628571295386
29	24.053131832018014	26.71135495960806	27.360935657248874	21.874577551125054
30	23.29190809377083	26.26338434573449	28.38669979442764	22.058007766067043
31	22.533015107937143	26.763563813670327	28.17203749714483	22.5313835812477
32	21.347827505675383	26.70809190622917	28.588776028677575	23.35530455941787
33	21.414953175184014	26.7950289712525	29.248145886455067	22.54187196710842
34	22.53394740890254	26.78360828442638	28.621406562466493	22.061037744204583
35	22.67752175757378	27.399859222554223	27.710781594514337	22.211837425357654
36	23.561109997529403	27.174475464169344	27.34322193890632	21.92119259939494
37	22.674491779436238	26.404394866750884	27.77790726402297	23.143206089789906
38	22.859320445826324	26.89734900220489	27.460458785305068	22.782871766663714
39	21.99064902131706	26.89105597068846	28.308619588575585	22.809675419418895
40	22.302736769483925	28.021237815991757	28.122625545978753	21.553399868545565
41	22.24609948583602	26.28482726793864	28.33006251077973	23.139010735445616
42	21.706996452594826	27.732690667201187	28.33425786512402	22.226055015079968
43	22.8478997590002	26.131929909613422	27.914023204971027	23.10614712641535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	45.5
2	51.0
3	171.5
4	292.0
5	292.0
6	355.0
7	418.0
8	418.0
9	418.0
10	538.5
11	659.0
12	659.0
13	1073.0
14	1487.0
15	2656.0
16	3825.0
17	4127.0
18	4429.0
19	4429.0
20	3869.5
21	3310.0
22	3319.5
23	3329.0
24	4183.5
25	5038.0
26	5038.0
27	6618.5
28	8199.0
29	9575.5
30	10952.0
31	13456.5
32	15961.0
33	15961.0
34	20269.0
35	24577.0
36	26522.5
37	28468.0
38	30355.5
39	32243.0
40	32243.0
41	33619.5
42	34996.0
43	35961.0
44	36926.0
45	35875.0
46	34824.0
47	34824.0
48	33172.0
49	31520.0
50	31492.0
51	31464.0
52	29804.0
53	28144.0
54	28144.0
55	27163.0
56	26182.0
57	22060.0
58	17938.0
59	16065.0
60	14192.0
61	14192.0
62	12394.5
63	10597.0
64	8406.0
65	6215.0
66	5109.5
67	4004.0
68	4004.0
69	3311.5
70	2619.0
71	2283.0
72	1947.0
73	1800.0
74	1653.0
75	1653.0
76	1407.0
77	1161.0
78	832.0
79	503.0
80	352.0
81	201.0
82	201.0
83	153.0
84	105.0
85	94.0
86	83.0
87	66.5
88	50.0
89	50.0
90	34.0
91	18.0
92	12.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	429046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.80812003306384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.73649875197799	41.846373223011696
2	7.298345177748187	7.1243701495653236
3	2.121474712877784	3.106355772997456
4	1.03554074617588	2.021711881539234
5	0.6290493591414169	1.535135831384808
6	0.44280641860351816	1.2967529298366975
7	0.34871367904591294	1.1914041372830955
8	0.2446690843698978	0.9553470430644636
9	0.21322733903559823	0.936650300218225
>10	1.566517439428604	15.361164980847853
>50	0.20515713126263965	7.005299914323573
>100	0.14681861052444492	13.227092044306021
>500	0.009723086789698339	2.9567808905729285
>1k	0.0014584630184547509	1.4355609010485688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2765	0.6444530423311253	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2194	0.5113670795206108	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1091	0.254285088312209	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	865	0.20161008376724174	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	832	0.19391860080271114	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	787	0.18343021494198758	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	769	0.17923486059769816	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	726	0.16921262521967342	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	630	0.14683740205012982	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	628	0.146371251567431	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	620	0.1445066496366357	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	614	0.14310819818853923	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	596	0.1389128438442498	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	581	0.1354167152240086	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	569	0.13261981232781567	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	564	0.1314544361210686	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	550	0.1281913827421768	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	539	0.12562755508733328	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	535	0.12469525412193563	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	529	0.12329680267383918	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	519	0.12096605026034504	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	504	0.11746992164010385	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	504	0.11746992164010385	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	497	0.11583839495065797	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	468	0.109079212951525	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	466	0.10861306246882618	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	462	0.10768076150342853	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	458	0.10674846053803089	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	441	0.10278618143509087	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	435	0.1013877299869944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.6615048269882484E-4	0.0	0.0
2	0.0	0.0	4.6615048269882484E-4	0.0	0.0
3	0.0	0.0	4.6615048269882484E-4	0.0	0.0
4	0.0	0.0	6.992257240482372E-4	2.3307524134941242E-4	0.0
5	0.0	0.0	6.992257240482372E-4	4.6615048269882484E-4	0.0
6	0.0	0.0	9.323009653976497E-4	4.6615048269882484E-4	0.0
7	0.0	0.0	0.0030299781375423616	4.6615048269882484E-4	0.0
8	0.0	0.0	0.0037292038615905987	4.6615048269882484E-4	0.0
9	0.0	0.0	0.0039622791029400115	6.992257240482372E-4	0.0
10	9.323009653976497E-4	0.0	0.004661504826988248	6.992257240482372E-4	2.3307524134941242E-4
11	9.323009653976497E-4	0.0	0.00675918199913296	9.323009653976497E-4	2.3307524134941242E-4
12	9.323009653976497E-4	0.0	0.006992257240482373	0.001165376206747062	2.3307524134941242E-4
13	9.323009653976497E-4	0.0	0.007691482964530609	0.001165376206747062	2.3307524134941242E-4
14	9.323009653976497E-4	0.0	0.008157633447229435	0.001165376206747062	2.3307524134941242E-4
15	9.323009653976497E-4	0.0	0.008390708688578847	0.001165376206747062	2.3307524134941242E-4
16	9.323009653976497E-4	0.0	0.008390708688578847	0.001165376206747062	2.3307524134941242E-4
17	9.323009653976497E-4	0.0	0.008390708688578847	0.001165376206747062	2.3307524134941242E-4
18	9.323009653976497E-4	0.0	0.008390708688578847	0.001165376206747062	2.3307524134941242E-4
19	9.323009653976497E-4	0.0	0.008623783929928259	0.0013984514480964744	2.3307524134941242E-4
20	0.001165376206747062	0.0	0.008856859171277673	0.001631526689445887	2.3307524134941242E-4
21	0.001165376206747062	0.0	0.009789160136675322	0.001631526689445887	2.3307524134941242E-4
22	0.001165376206747062	0.0	0.009789160136675322	0.0025638276548435365	2.3307524134941242E-4
23	0.001165376206747062	0.0	0.009789160136675322	0.0030299781375423616	2.3307524134941242E-4
24	0.001165376206747062	0.0	0.009789160136675322	0.0037292038615905987	2.3307524134941242E-4
25	0.001165376206747062	0.0	0.009789160136675322	0.0037292038615905987	2.3307524134941242E-4
26	0.001165376206747062	0.0	0.009789160136675322	0.004661504826988248	2.3307524134941242E-4
27	0.001165376206747062	0.0	0.009789160136675322	0.005593805792385898	2.3307524134941242E-4
28	0.001165376206747062	0.0	0.009789160136675322	0.017713718342555345	2.3307524134941242E-4
29	0.0013984514480964744	0.0	0.010022235378024734	0.03822433958130363	2.3307524134941242E-4
30	0.0013984514480964744	0.0	0.010022235378024734	0.07038872288752256	2.3307524134941242E-4
31	0.0013984514480964744	0.0	0.010022235378024734	0.13681516667210508	2.3307524134941242E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGAC	20	0.0018406502	37.0	31
TACCCGA	25	0.0054925526	29.599998	30
ATTCGTG	25	0.0054925526	29.599998	11
GTCGGAA	25	0.0054925526	29.599998	21
ATTGTAC	35	8.860299E-4	26.42857	3
CCGCTCT	35	8.860299E-4	26.42857	28
GGTATCA	495	0.0	23.171717	1
TCACGGT	40	0.001929225	23.125002	1
CGTGCAG	40	0.001929225	23.125002	14
CTTAGGA	40	0.001929225	23.125002	2
ATACCAT	40	0.001929225	23.125002	6
TCTATAC	65	2.6763428E-6	22.769228	3
GTATCAA	1320	0.0	22.704546	1
AGGACCG	50	2.698279E-4	22.199999	5
TGCTCGC	50	2.698279E-4	22.199999	10
GGACCGT	50	2.698279E-4	22.199999	6
AGCTTCG	70	5.0896324E-6	21.142857	21
ATTGACG	80	6.939208E-7	20.8125	17
TCGGAAC	45	0.0038219588	20.555555	22
ACGGTAC	45	0.0038219588	20.555555	3
>>END_MODULE