FastQCFastQC Report
Fri 10 Feb 2017
ERR1633510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336786
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93782.7845575528673994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73122.171111625780169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37271.1066374493001492No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20940.6217598118686644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.5534671868783144No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17870.5306040037293712No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15160.4501374760233501No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13610.40411418526898385No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13450.3993633939653073No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12230.3631386102747739No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC12110.3595755167970165No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10030.29781522984922176No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9670.2871259494159496No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.2871259494159496No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9150.2716858776790009No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9100.270201255396602No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9000.2672320108318042No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8820.2618873706151681No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8480.2517919390948555No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8010.23783648964030574No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7730.22952260485887183No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7380.2191302488820794No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7340.2179425510561603No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7340.2179425510561603No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7330.2176456265996805No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7240.21497330649136248No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7110.2111132885571253No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7080.21022251518768592No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7040.20903481736176682No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7020.20844096844880727No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7000.2078471195358477No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6990.2075501950793679No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6930.2057686483404892No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6890.2045809505145701No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6480.192407047798899No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6320.18765625649522247No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5800.1722161847582738No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5800.1722161847582738No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.171919260301794No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5760.17102848693235467No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5740.1704346380193951No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.16984078910643555No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5630.1671684689981175No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5620.16687154454163772No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5600.16627769562867814No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5510.1636053755203601No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5510.1636053755203601No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5460.16212075323796118No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5400.16033920649908248No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5400.16033920649908248No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5380.15974535758612293No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5330.15826073530372403No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5280.15677611302132513No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5250.15588533965188578No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5120.1520253217176486No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5090.15113454834820925No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.15083762389172947No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5050.14994685052229012No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5030.14935300160933057No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA5010.148759152696371No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4890.14519605921861362No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4890.14519605921861362No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4880.1448991347621338No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4870.14460221030565404No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4840.1437114369362147No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4810.14282066356677534No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4680.13896064563253815No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4660.1383667967195786No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4560.13539755215478078No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4560.13539755215478078No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4380.1300529119381447No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4340.12886521411222557No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4240.12589596954742774No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4230.12559904509094796No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4230.12559904509094796No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4220.1253021206344682No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4200.12470827172150863No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4200.12470827172150863No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4100.12173902715671078No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4080.12114517824375122No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4010.11906670704839276No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3960.11758208476599383No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3910.11609746248359493No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3850.11431591574471622No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3740.11104974672343862No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3660.10867435107160037No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3600.10689280433272166No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3590.10659587987624189No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3590.10659587987624189No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3560.10570510650680254No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3550.10540818205032275No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3500.10392355976792385No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3470.1030327863984845No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3440.10214201302904515No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3410.1012512396596058No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3400.10095431520312603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTT251.2317729E-437.08
ATAGATC251.2317729E-437.06
TTATAGA251.2317729E-437.04
TATAGAT251.2317729E-437.05
TGACATC352.3833329E-531.71428517
TATTATA352.3833329E-531.7142852
TCTTGGT303.5941234E-430.83333211
GATCTTG303.5941234E-430.8333329
TGGTGAC303.5941234E-430.83333214
TGTCCGC303.5941234E-430.83333214
CGCGAGT250.00549089529.618
GAGGTGC250.00549089529.621
ATTGTTA453.9972E-628.77777914
TATTGTT453.9972E-628.77777913
CACTGTG754.6020432E-1027.1333337
CTGTGCA754.6020432E-1027.1333339
ATCTTGG358.856325E-426.4285710
GACATCT358.856325E-426.4285718
ATTATAG358.856325E-426.428573
GTGACAT358.856325E-426.4285716
Produced by FastQC (version 0.11.5)