FastQCFastQC Report
Fri 10 Feb 2017
ERR1633509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216269
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47912.215296690695384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42991.987802227781143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20780.9608404348288474No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15450.7143881000050862No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12950.5987913200689882No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10940.5058515090003652No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10510.48596886285135643No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10000.4623871197443924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9460.4374182152781952No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7720.35696285644267095No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7580.35048943676624944No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6880.31812233838414194No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6680.30887459598925415No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6440.2977773051153887No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6370.29454059527717796No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6350.2936158210376892No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6060.2802065945651018No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5970.27604511048740227No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5930.2741955620084247No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5750.26587259385302564No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5640.2607863355358373No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5630.2603239484160929No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.255700077218649No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5490.25385052873967145No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5430.25107620602120506No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5370.24830188330273872No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5340.24691472194350553No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4840.2237953659562859No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4830.22333297883654152No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4790.22148343035756396No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4680.21639717204037565No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4620.21362284932190928No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4570.2113109137231873No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4510.20853659100472094No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4330.2002136228493219No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4290.19836407437034434No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4260.19697691301111117No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4230.19558975165187797No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4120.19050349333468966No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4080.1886539448557121No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4070.1881915577359677No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4010.18541723501750138No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3850.17801904110159109No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3840.17755665398184667No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3820.1766318797423579No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3790.17524471838312472No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3770.17431994414363594No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3760.17385755702389152No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3730.17247039566465838No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC3680.1701584600659364No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3670.16969607294619202No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3650.16877129870670324No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3650.16877129870670324No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3640.16830891158695882No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3630.16784652446721443No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3550.16414742750925929No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3450.1595235563118154No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3440.15906116919207097No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3440.15906116919207097No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3400.15721162071309341No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3300.1525877495156495No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3230.14935103967743873No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3230.14935103967743873No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3170.1465767169589724No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3120.14426478136025042No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3100.14334000712076164No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3090.14287762000101725No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.14056568440229528No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3040.14056568440229528No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3040.14056568440229528No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3000.13871613592331772No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3000.13871613592331772No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2990.13825374880357333No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2960.13686658744434013No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2920.13501703896536257No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2880.13316749048638502No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2830.13085555488766304No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2790.12900600640868548No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA2790.12900600640868548No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2790.12900600640868548No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2790.12900600640868548No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2780.1285436192889411No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2770.1280812321691967No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2700.12484452233098595No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2680.12391974809149717No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC2660.12299497385200837No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2640.12207019961251961No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT2630.12160781249277519No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2620.1211454253730308No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC2580.11929587689405324No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2560.11837110265456446No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2530.11698394129533128No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2460.11374723145712053No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.11189768297814295No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.11143529585839858No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2390.11051052161890977No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2370.109585747379421No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT2340.10819858602018782No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG2340.10819858602018782No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG2320.10727381178069904No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA2290.10588665042146586No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG2290.10588665042146586No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2280.10542426330172147No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA2280.10542426330172147No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA2280.10542426330172147No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2240.10357471482274391No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG2210.10218755346351072No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA2190.10126277922402192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT251.2303142E-436.9999961
TTATAGA250.005486604329.5999984
ATTATAG250.005486604329.5999983
GATCTTG250.005486604329.5999989
ACCCCAC250.005486604329.59999830
ATAGATC250.005486604329.5999986
TCTTGGT250.005486604329.59999811
TATTATA250.005486604329.5999982
TGGTGAC250.005486604329.59999814
ACTAGTG509.046606E-625.8999988
CCGCCGC451.3183652E-424.66666632
GAGTCGC751.3606041E-824.66666622
GCGAGTC751.3606041E-824.66666620
TCCGCCG551.8934144E-523.54545431
TATACAC400.001926148423.12500237
GGCGAGT802.699926E-823.12500219
AGTCGCC802.699926E-823.12500223
CGCCGTC753.7117934E-722.226
CCGTCAA502.6922853E-422.19999923
CGGGCCC502.6922853E-422.19999916