FastQCFastQC Report
Fri 10 Feb 2017
ERR1633508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441856
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26550.6008744930475086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.42638325608342986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9580.2168127172653534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.1131590382387022No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4610.10433263325608344No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4550.102974724797219No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4520.10229577056778678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT509.074558E-625.90000228
CGCTCTC601.334296E-624.66666829
TTCGCCG601.334296E-624.66666824
GCTCCGA952.8012437E-1023.36842229
TGCTCCG1155.456968E-1222.52173828
GTATCAA11000.022.0318161
GCCGCTC603.719607E-521.58333427
GTCGCCC957.1140676E-921.42105337
GCCGGCA705.0902017E-621.14285715
GGTATCA4650.021.0860211
TATACTG450.003822137820.5555555
AATACCC450.003822137820.5555555
TGCTCGC759.244619E-619.73333410
CGCCGGC851.2417622E-619.58823414
TAGGACC1052.249908E-819.380954
CCGGCAG902.146122E-618.516
ATAGCTC500.007028196518.53
ATTACAC609.2245534E-418.53
GCTTCGC801.6130032E-518.522
CGGAGTT500.007028196518.524
Produced by FastQC (version 0.11.5)