##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633508.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441856
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.889396092844727	33.0	33.0	33.0	33.0	33.0
2	32.01576305402665	33.0	33.0	33.0	33.0	33.0
3	31.730826332560834	33.0	33.0	33.0	27.0	33.0
4	32.00567153099652	33.0	33.0	33.0	33.0	33.0
5	32.10391847117613	33.0	33.0	33.0	33.0	33.0
6	35.29869459733488	37.0	37.0	37.0	33.0	37.0
7	35.42747637239282	37.0	37.0	37.0	33.0	37.0
8	35.55833348421205	37.0	37.0	37.0	33.0	37.0
9	35.65971492975087	37.0	37.0	37.0	33.0	37.0
10	35.68190994351101	37.0	37.0	37.0	33.0	37.0
11	35.685037659327925	37.0	37.0	37.0	33.0	37.0
12	35.69843568945539	37.0	37.0	37.0	33.0	37.0
13	35.68705867975087	37.0	37.0	37.0	33.0	37.0
14	35.661514158458864	37.0	37.0	37.0	33.0	37.0
15	35.67593288311124	37.0	37.0	37.0	33.0	37.0
16	35.66246695756083	37.0	37.0	37.0	33.0	37.0
17	35.659074449594435	37.0	37.0	37.0	33.0	37.0
18	35.627727132821555	37.0	37.0	37.0	33.0	37.0
19	35.64057068366164	37.0	37.0	37.0	33.0	37.0
20	35.649917620220165	37.0	37.0	37.0	33.0	37.0
21	35.648496342699886	37.0	37.0	37.0	33.0	37.0
22	35.562461525927	37.0	37.0	37.0	33.0	37.0
23	35.61313640643105	37.0	37.0	37.0	33.0	37.0
24	35.58159671929317	37.0	37.0	37.0	33.0	37.0
25	35.60514964151216	37.0	37.0	37.0	33.0	37.0
26	35.50238539252607	37.0	37.0	37.0	33.0	37.0
27	35.5100417330533	37.0	37.0	37.0	33.0	37.0
28	35.526519952201625	37.0	37.0	37.0	33.0	37.0
29	35.545170824884124	37.0	37.0	37.0	33.0	37.0
30	35.54078251738123	37.0	37.0	37.0	33.0	37.0
31	35.52187816845307	37.0	37.0	37.0	33.0	37.0
32	35.516000688006955	37.0	37.0	37.0	33.0	37.0
33	35.50599742902665	37.0	37.0	37.0	33.0	37.0
34	35.48072675260718	37.0	37.0	37.0	33.0	37.0
35	35.41606541497683	37.0	37.0	37.0	33.0	37.0
36	35.43737326187717	37.0	37.0	37.0	33.0	37.0
37	35.442483524044036	37.0	37.0	37.0	33.0	37.0
38	35.41814075173812	37.0	37.0	37.0	33.0	37.0
39	35.31378096031286	37.0	37.0	37.0	33.0	37.0
40	35.112719981170336	37.0	37.0	37.0	33.0	37.0
41	35.26461335819815	37.0	37.0	37.0	33.0	37.0
42	35.31662804171495	37.0	37.0	37.0	33.0	37.0
43	34.98643675767671	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	15.0
16	16.0
17	25.0
18	14.0
19	12.0
20	34.0
21	75.0
22	175.0
23	477.0
24	898.0
25	1679.0
26	2718.0
27	4190.0
28	5932.0
29	7932.0
30	10344.0
31	13578.0
32	17019.0
33	22524.0
34	33474.0
35	68053.0
36	252670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12393177867903	22.08864426419467	14.38862434820394	23.398799608922364
2	15.203595741599074	22.539922508690612	40.15833212630359	22.09814962340672
3	17.065740874855155	28.555230663383547	31.246152592699882	23.132875869061415
4	12.023826767091542	18.407128114136732	40.94071371668598	28.62833140208575
5	12.675849145422942	38.48719944959444	36.03934313441483	12.797608270567787
6	27.566220669177287	41.4082868626883	18.12830424391657	12.897188224217846
7	24.57271147161066	33.44370111529548	24.745844800115872	17.237742612977982
8	23.40400492468134	36.63569126593279	21.569923232908458	18.390380576477405
9	25.039831981460026	16.00091432502897	21.34654729142526	37.61270640208575
10	13.900003621089224	29.064672653534185	34.955279548088065	22.08004417728853
11	31.797010790845885	25.058842699884128	25.312771581691774	17.831374927578217
12	21.51922798377752	27.119695104287374	31.404575246234067	19.956501665701044
13	26.566347407300118	23.477105663383547	27.810870509849362	22.145676419466977
14	21.096013180764775	22.2045191193511	29.291443366164543	27.40802433371958
15	22.752208864426418	29.884623044611818	26.070031865585168	21.293136225376593
16	21.6656557792584	30.172499637891075	26.002362760718423	22.159481822132097
17	21.15734537949015	28.93612398609502	27.63230554750869	22.274225086906142
18	21.027438803592123	28.505893322711472	29.575246234067208	20.8914216396292
19	23.184250072421783	27.603563151796063	29.62209407589803	19.590092699884128
20	22.798151433951332	27.1864589368482	29.68772631807648	20.327663311123985
21	21.1878983198146	28.340228490730013	29.16131047219003	21.310562717265352
22	20.880784690034762	28.507024913093858	28.943592482618776	21.668597914252608
23	20.919711399188877	28.190858560254924	29.553746016801853	21.335684023754347
24	21.665203143105447	28.590762601390495	28.42690831402086	21.317125941483198
25	21.622429026651215	28.056651940903826	29.238032300115872	21.082886732329083
26	21.486638180764775	28.121831546929315	29.298006590382386	21.093523681923525
27	21.605228852838934	28.022477911355736	28.599815324449594	21.772477911355736
28	20.7642308806489	28.228427360950175	29.544014339513325	21.4633274188876
29	22.26924608922364	28.318954591541136	28.61316809096176	20.798631228273464
30	21.320973348783316	28.39726064600232	29.178058009849362	21.103707995365006
31	21.471701187717265	27.979930112977986	29.233732256662805	21.314636442641945
32	20.47092265353418	28.00640932792584	29.4331184820394	22.08954953650058
33	20.78256264484357	27.848212992468135	29.99981894553882	21.36940541714948
34	21.078360370799537	27.819017960602547	29.510066628041713	21.5925550405562
35	21.428700753186558	28.598910052143683	28.786527737543455	21.185861457126304
36	21.728798522595596	28.363991888760136	28.803501593279258	21.103707995365006
37	21.70480880648899	27.549925767670914	28.691247827346466	22.054017598493626
38	21.805746668597912	28.11209986964079	28.815722769409035	21.26643069235226
39	21.23587775202781	28.053030851680184	29.17172110370799	21.53937029258401
40	21.26009378621089	28.592799464078794	29.049509342410197	21.097597407300118
41	21.11434494495944	27.91022414542294	29.292122320393975	21.68330858922364
42	21.233840889339515	28.275954157010432	28.942460892236383	21.547744061413674
43	21.366236964078794	27.68752715816918	28.987271871378912	21.958964006373115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	57.5
2	61.0
3	197.0
4	333.0
5	333.0
6	388.0
7	443.0
8	409.0
9	375.0
10	590.5
11	806.0
12	806.0
13	1218.5
14	1631.0
15	2997.0
16	4363.0
17	5038.0
18	5713.0
19	5713.0
20	5588.5
21	5464.0
22	6482.5
23	7501.0
24	9009.0
25	10517.0
26	10517.0
27	12228.0
28	13939.0
29	16596.5
30	19254.0
31	20895.5
32	22537.0
33	22537.0
34	25008.0
35	27479.0
36	28681.0
37	29883.0
38	31229.0
39	32575.0
40	32575.0
41	33564.0
42	34553.0
43	33351.5
44	32150.0
45	31645.0
46	31140.0
47	31140.0
48	29808.5
49	28477.0
50	28783.0
51	29089.0
52	28340.0
53	27591.0
54	27591.0
55	24930.5
56	22270.0
57	19245.0
58	16220.0
59	14220.0
60	12220.0
61	12220.0
62	10627.0
63	9034.0
64	7358.5
65	5683.0
66	4769.5
67	3856.0
68	3856.0
69	3166.5
70	2477.0
71	2131.5
72	1786.0
73	1434.5
74	1083.0
75	1083.0
76	798.0
77	513.0
78	412.5
79	312.0
80	231.0
81	150.0
82	150.0
83	147.5
84	145.0
85	121.0
86	97.0
87	75.0
88	53.0
89	53.0
90	38.0
91	23.0
92	13.5
93	4.0
94	2.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	441856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.276063976917314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.44135031661375	54.80613904182698
2	5.889812072853908	7.218090027764322
3	1.4779230383736885	2.716839199570385
4	0.7212175234090026	1.7677348442273564
5	0.44664649457715905	1.3684369588387926
6	0.2921863839640164	1.074241893417792
7	0.20079429709761748	0.8612718936607626
8	0.15599493385685032	0.7647004437669876
9	0.12326534284654893	0.6797893530961602
>10	1.0253478368816722	12.843331678804018
>50	0.15392821124087652	6.673393424567943
>100	0.0704099846992322	7.964514556492516
>500	3.7452119520868196E-4	0.21985318960149347
>1k	7.490423904173639E-4	1.0416634943644874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2655	0.6008744930475086	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1884	0.42638325608342986	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	958	0.2168127172653534	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.1131590382387022	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	461	0.10433263325608344	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	455	0.102974724797219	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	452	0.10229577056778678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	2.2631807647740442E-4	0.0	0.0
7	0.0	0.0	6.789542294322132E-4	0.0	0.0
8	0.0	0.0	6.789542294322132E-4	0.0	0.0
9	0.0	0.0	6.789542294322132E-4	2.2631807647740442E-4	0.0
10	0.0	0.0	9.052723059096177E-4	2.2631807647740442E-4	0.0
11	0.0	0.0	0.003394771147161066	4.5263615295480883E-4	0.0
12	0.0	0.0	0.004073725376593279	4.5263615295480883E-4	0.0
13	0.0	0.0	0.004073725376593279	4.5263615295480883E-4	0.0
14	0.0	0.0	0.004073725376593279	4.5263615295480883E-4	0.0
15	0.0	0.0	0.004300043453070683	6.789542294322132E-4	0.0
16	0.0	0.0	0.004978997682502897	9.052723059096177E-4	0.0
17	0.0	0.0	0.005205315758980302	9.052723059096177E-4	0.0
18	0.0	0.0	0.005205315758980302	0.0013579084588644264	0.0
19	0.0	0.0	0.005205315758980302	0.0015842265353418308	0.0
20	0.0	0.0	0.005431633835457706	0.0015842265353418308	0.0
21	0.0	0.0	0.00565795191193511	0.0018105446118192353	0.0
22	0.0	0.0	0.00565795191193511	0.0020368626882966397	0.0
23	0.0	0.0	0.00565795191193511	0.0024894988412514484	0.0
24	0.0	0.0	0.00565795191193511	0.004300043453070683	0.0
25	0.0	0.0	0.00565795191193511	0.004752679606025492	0.0
26	0.0	0.0	0.00565795191193511	0.004752679606025492	0.0
27	0.0	0.0	0.00565795191193511	0.006336906141367323	0.0
28	0.0	0.0	0.00565795191193511	0.01380540266512167	0.0
29	0.0	0.0	0.00565795191193511	0.03394771147161066	0.0
30	0.0	0.0	0.00565795191193511	0.05974797219003476	0.0
31	0.0	0.0	0.00565795191193511	0.11066953939745075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTCT	50	9.074558E-6	25.900002	28
CGCTCTC	60	1.334296E-6	24.666668	29
TTCGCCG	60	1.334296E-6	24.666668	24
GCTCCGA	95	2.8012437E-10	23.368422	29
TGCTCCG	115	5.456968E-12	22.521738	28
GTATCAA	1100	0.0	22.031816	1
GCCGCTC	60	3.719607E-5	21.583334	27
GTCGCCC	95	7.1140676E-9	21.421053	37
GCCGGCA	70	5.0902017E-6	21.142857	15
GGTATCA	465	0.0	21.086021	1
TATACTG	45	0.0038221378	20.555555	5
AATACCC	45	0.0038221378	20.555555	5
TGCTCGC	75	9.244619E-6	19.733334	10
CGCCGGC	85	1.2417622E-6	19.588234	14
TAGGACC	105	2.249908E-8	19.38095	4
CCGGCAG	90	2.146122E-6	18.5	16
ATAGCTC	50	0.0070281965	18.5	3
ATTACAC	60	9.2245534E-4	18.5	3
GCTTCGC	80	1.6130032E-5	18.5	22
CGGAGTT	50	0.0070281965	18.5	24
>>END_MODULE