FastQCFastQC Report
Fri 10 Feb 2017
ERR1633507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515469
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25520.49508311848045183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.35850846510653406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9980.19361009100450272No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7190.13948462468160064No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7030.13638065528673887No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7020.13618665719956002No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6890.13366468206623483No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6160.11950282170217802No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT5860.1136828790868122No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5830.11310088482527562No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5800.11251889056373904No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5640.10941492116887729No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.10844493073298297No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5450.10572895751247893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGG250.005493565529.62
TTCGCCG659.370888E-825.61538524
GGTATCA4000.024.51251
GCGGGTA400.0019297523.12500222
GTATCAA9950.022.8693471
TGCGGGT450.003822988820.55555521
AGAGCCG450.003822988820.5555555
TGCGGTA555.1378063E-420.18181836
CATGCTA2750.019.5090924
CGAACTA2800.018.529
TTTCGTC500.00702974618.517
TGTATTG801.6138529E-518.55
GCCCTAA500.00702974618.520
GCGAACT2900.017.86206828
GCCGGCA953.598585E-617.52631615
TAGGCTG852.7183993E-517.4117645
TACATGC3000.017.2666662
GTACATG3250.017.0769231
GTATTGG650.001578335917.0769231
GTACTCA650.001578335917.0769231
Produced by FastQC (version 0.11.5)