##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633507.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515469
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.820031854485915	33.0	33.0	33.0	27.0	33.0
2	31.95088550426893	33.0	33.0	33.0	33.0	33.0
3	31.67511916332505	33.0	33.0	33.0	27.0	33.0
4	31.952495688392514	33.0	33.0	33.0	33.0	33.0
5	32.05129309425009	33.0	33.0	33.0	33.0	33.0
6	35.20837916538143	37.0	37.0	37.0	33.0	37.0
7	35.34405366763084	37.0	37.0	37.0	33.0	37.0
8	35.46412490372845	37.0	37.0	37.0	33.0	37.0
9	35.579567345465975	37.0	37.0	37.0	33.0	37.0
10	35.59272235575757	37.0	37.0	37.0	33.0	37.0
11	35.60026306140622	37.0	37.0	37.0	33.0	37.0
12	35.609951325879926	37.0	37.0	37.0	33.0	37.0
13	35.598026263461044	37.0	37.0	37.0	33.0	37.0
14	35.588549456902356	37.0	37.0	37.0	33.0	37.0
15	35.59596988373695	37.0	37.0	37.0	33.0	37.0
16	35.57709774981619	37.0	37.0	37.0	33.0	37.0
17	35.5663075762073	37.0	37.0	37.0	33.0	37.0
18	35.56434819552679	37.0	37.0	37.0	33.0	37.0
19	35.55552128256016	37.0	37.0	37.0	33.0	37.0
20	35.5531525659157	37.0	37.0	37.0	33.0	37.0
21	35.553841259125186	37.0	37.0	37.0	33.0	37.0
22	35.46537813137162	37.0	37.0	37.0	33.0	37.0
23	35.50649408596831	37.0	37.0	37.0	33.0	37.0
24	35.49466408261215	37.0	37.0	37.0	33.0	37.0
25	35.522108992005336	37.0	37.0	37.0	33.0	37.0
26	35.4040747358231	37.0	37.0	37.0	33.0	37.0
27	35.424902370462625	37.0	37.0	37.0	33.0	37.0
28	35.44162694555832	37.0	37.0	37.0	33.0	37.0
29	35.43502519065162	37.0	37.0	37.0	33.0	37.0
30	35.44476195464713	37.0	37.0	37.0	33.0	37.0
31	35.43475359332957	37.0	37.0	37.0	33.0	37.0
32	35.42155396347792	37.0	37.0	37.0	33.0	37.0
33	35.41171244051534	37.0	37.0	37.0	33.0	37.0
34	35.390613208553766	37.0	37.0	37.0	33.0	37.0
35	35.33595230751025	37.0	37.0	37.0	33.0	37.0
36	35.3563997058989	37.0	37.0	37.0	33.0	37.0
37	35.34418558633012	37.0	37.0	37.0	33.0	37.0
38	35.34605960785227	37.0	37.0	37.0	33.0	37.0
39	35.237511858133075	37.0	37.0	37.0	33.0	37.0
40	35.01686619369933	37.0	37.0	37.0	27.0	37.0
41	35.19365471056455	37.0	37.0	37.0	33.0	37.0
42	35.23270264555192	37.0	37.0	37.0	33.0	37.0
43	34.90978118955747	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	19.0
17	25.0
18	15.0
19	21.0
20	37.0
21	97.0
22	250.0
23	677.0
24	1262.0
25	2167.0
26	3503.0
27	5275.0
28	7491.0
29	10143.0
30	12949.0
31	16600.0
32	20848.0
33	27667.0
34	39496.0
35	79881.0
36	287034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53842229115621	21.564633372714944	13.638065528673888	24.25887880745496
2	15.822677988395034	21.800729044811618	38.8232852024079	23.55330776438544
3	17.22819413000588	27.67712510354648	30.198518242610128	24.896162523837514
4	12.082394867586606	18.20361651234119	40.50777059338195	29.206218026690255
5	13.358708283136329	37.89306437438527	35.701273985438505	13.0469533570399
6	28.706090957943154	40.835239364539866	17.706399414901767	12.752270262615212
7	25.93036632658802	32.73543122864809	24.084280528993986	17.24992191576991
8	24.03209504354287	36.404323053374696	20.81968071794812	18.743901185134316
9	25.626759320153102	15.563690542011255	20.873806184271025	37.93574395356462
10	14.722126839829357	28.340598561698183	34.3679251322582	22.569349466214263
11	33.24816817306181	24.12734810434769	24.76055786089949	17.863925861691005
12	22.39358719923022	26.27432493515614	30.83153400107475	20.500553864538894
13	27.01947158801014	22.85433265628001	26.715476585400864	23.41071917030898
14	21.445324549099944	21.435042650479467	28.608315922005005	28.511316878415577
15	23.445056831739638	29.32494485604372	25.2973505681234	21.93264774409324
16	22.489810250470928	29.120858868331556	25.61337345213776	22.775957429059748
17	21.726427777422114	28.312468839057246	27.011517666435807	22.94958571708483
18	21.875806304549837	28.249613458811297	28.57068029309231	21.303899943546554
19	23.840618931497335	27.161672185912245	28.130886629457834	20.866822253132582
20	23.190531341360973	26.817131583082592	28.588916113287123	21.403420962269312
21	22.211422995369265	27.56848617472632	28.642847581522844	21.57724324838157
22	21.91615790668304	27.506600784916262	28.324302722375155	22.252938586025543
23	22.088816204272227	27.33122651410657	28.78155621385573	21.79840106776547
24	22.496212187347833	27.96172029743787	27.243733376788903	22.298334138425396
25	22.378455348430265	27.62571561044408	28.235645596534418	21.760183444591235
26	22.303572086779226	27.85618533801257	28.42770370284149	21.412538872366717
27	22.270786410045996	27.92253268382774	27.685855017469528	22.120825888656736
28	21.812756926216707	27.577798082910903	28.59958600808196	22.00985898279043
29	23.461158672975486	27.38612797277819	27.54966836026997	21.603044993976358
30	22.736381819275262	26.998325796507643	28.329346672641808	21.935945711575283
31	22.256818547769118	27.29941082780924	28.23719758123185	22.206573043189795
32	21.54833753339192	27.236749445650467	28.521404778948877	22.693508242008733
33	21.44590654336148	27.30542476851178	29.050437562685634	22.198231125441104
34	22.303572086779226	27.05943519396899	28.73208670162512	21.904906017626665
35	22.253714578374257	27.817773716751155	27.922920680002093	22.005591024872494
36	23.158327658889284	27.506794783003443	27.602241841895435	21.73263571621184
37	22.44266871528647	26.95137825941036	27.78906199984868	22.81689102545449
38	22.50164413378884	27.41464569159348	27.84532144513055	22.238388729487127
39	21.98619121615461	27.39466388861406	28.16735826984746	22.451786625383875
40	22.015678925405798	28.140392535729596	28.105278881950223	21.738649656914383
41	21.985221225718714	26.853603223472216	28.442835553641444	22.71833999716763
42	21.808682966385952	27.931650593925145	28.234093611836986	22.025572827851917
43	22.543741718706652	26.763588111021225	28.055421373545258	22.637248796726865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	47.5
2	63.0
3	199.5
4	336.0
5	336.0
6	411.5
7	487.0
8	430.0
9	373.0
10	496.0
11	619.0
12	619.0
13	1047.0
14	1475.0
15	2596.0
16	3717.0
17	4325.5
18	4934.0
19	4934.0
20	4862.0
21	4790.0
22	5266.0
23	5742.0
24	7276.5
25	8811.0
26	8811.0
27	11036.5
28	13262.0
29	15107.5
30	16953.0
31	19853.0
32	22753.0
33	22753.0
34	26572.0
35	30391.0
36	32727.0
37	35063.0
38	36551.5
39	38040.0
40	38040.0
41	39179.5
42	40319.0
43	41146.5
44	41974.0
45	40509.0
46	39044.0
47	39044.0
48	37521.0
49	35998.0
50	35461.0
51	34924.0
52	32876.5
53	30829.0
54	30829.0
55	29619.5
56	28410.0
57	24643.0
58	20876.0
59	19169.0
60	17462.0
61	17462.0
62	15330.5
63	13199.0
64	10940.5
65	8682.0
66	7311.0
67	5940.0
68	5940.0
69	4953.0
70	3966.0
71	3257.0
72	2548.0
73	2026.5
74	1505.0
75	1505.0
76	1153.0
77	801.0
78	624.0
79	447.0
80	388.0
81	329.0
82	329.0
83	245.0
84	161.0
85	136.0
86	111.0
87	93.0
88	75.0
89	75.0
90	48.0
91	21.0
92	13.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	515469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.428546264841714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.60147595265174	55.564342703311894
2	7.495186722143339	9.50817595538192
3	1.9149780927185847	3.6439282965047726
4	0.8311824424824048	2.108827760300774
5	0.4588219420584191	1.4551204389588477
6	0.29745770752156725	1.132038597801929
7	0.2160301406616205	0.9591734440089098
8	0.16327962152127068	0.8285271222214209
9	0.11216422136403637	0.6402972153643854
>10	0.7368746047184552	9.002511434858347
>50	0.10036935582409484	4.498250028416327
>100	0.06784224891525965	8.252981081269912
>500	0.0037173835022060063	1.5412690729365548
>1k	6.195639170343343E-4	0.8645568486640541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2552	0.49508311848045183	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1848	0.35850846510653406	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	998	0.19361009100450272	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	719	0.13948462468160064	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	703	0.13638065528673887	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	702	0.13618665719956002	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	689	0.13366468206623483	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	616	0.11950282170217802	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	586	0.1136828790868122	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	583	0.11310088482527562	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	580	0.11251889056373904	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	564	0.10941492116887729	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.10844493073298297	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	545	0.10572895751247893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.8799617435772085E-4	0.0	0.0
2	0.0	0.0	3.8799617435772085E-4	0.0	0.0
3	0.0	0.0	3.8799617435772085E-4	0.0	0.0
4	0.0	0.0	3.8799617435772085E-4	0.0	0.0
5	0.0	0.0	7.759923487154417E-4	0.0	0.0
6	0.0	0.0	0.0015519846974308834	0.0	0.0
7	0.0	0.0	0.0025219751333251856	0.0	0.0
8	0.0	0.0	0.003103969394861767	0.0	0.0
9	0.0	0.0	0.003685963656398348	9.699904358943021E-4	0.0
10	1.9399808717886043E-4	0.0	0.004073959830756068	0.0011639885230731626	0.0
11	1.9399808717886043E-4	0.0	0.005819942615365813	0.0015519846974308834	0.0
12	1.9399808717886043E-4	0.0	0.006595934964081254	0.0015519846974308834	0.0
13	1.9399808717886043E-4	0.0	0.006789933051260115	0.0015519846974308834	0.0
14	1.9399808717886043E-4	0.0	0.006983931138438975	0.0017459827846097438	0.0
15	3.8799617435772085E-4	0.0	0.007371927312796696	0.0017459827846097438	0.0
16	3.8799617435772085E-4	0.0	0.007565925399975556	0.0021339789589674644	0.0
17	3.8799617435772085E-4	0.0	0.007953921574333276	0.0021339789589674644	0.0
18	3.8799617435772085E-4	0.0	0.007953921574333276	0.0021339789589674644	0.0
19	3.8799617435772085E-4	0.0	0.008147919661512137	0.0021339789589674644	0.0
20	3.8799617435772085E-4	0.0	0.008729913923048718	0.0021339789589674644	0.0
21	3.8799617435772085E-4	0.0	0.008729913923048718	0.002327977046146325	0.0
22	3.8799617435772085E-4	0.0	0.008729913923048718	0.0029099713076829064	0.0
23	3.8799617435772085E-4	0.0	0.008923912010227578	0.003685963656398348	0.0
24	3.8799617435772085E-4	0.0	0.008923912010227578	0.00465595409229265	0.0
25	3.8799617435772085E-4	0.0	0.008923912010227578	0.005431946441008092	0.0
26	3.8799617435772085E-4	0.0	0.008923912010227578	0.006013940702544673	0.0
27	3.8799617435772085E-4	0.0	0.008923912010227578	0.006401936876902394	0.0
28	3.8799617435772085E-4	0.0	0.008923912010227578	0.016683835497381994	0.0
29	3.8799617435772085E-4	0.0	0.008923912010227578	0.035113653779373734	0.0
30	3.8799617435772085E-4	0.0	0.008923912010227578	0.062079387897235334	0.0
31	3.8799617435772085E-4	0.0	0.008923912010227578	0.1210548063996089	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAGG	25	0.0054935655	29.6	2
TTCGCCG	65	9.370888E-8	25.615385	24
GGTATCA	400	0.0	24.5125	1
GCGGGTA	40	0.00192975	23.125002	22
GTATCAA	995	0.0	22.869347	1
TGCGGGT	45	0.0038229888	20.555555	21
AGAGCCG	45	0.0038229888	20.555555	5
TGCGGTA	55	5.1378063E-4	20.181818	36
CATGCTA	275	0.0	19.509092	4
CGAACTA	280	0.0	18.5	29
TTTCGTC	50	0.007029746	18.5	17
TGTATTG	80	1.6138529E-5	18.5	5
GCCCTAA	50	0.007029746	18.5	20
GCGAACT	290	0.0	17.862068	28
GCCGGCA	95	3.598585E-6	17.526316	15
TAGGCTG	85	2.7183993E-5	17.411764	5
TACATGC	300	0.0	17.266666	2
GTACATG	325	0.0	17.076923	1
GTATTGG	65	0.0015783359	17.076923	1
GTACTCA	65	0.0015783359	17.076923	1
>>END_MODULE