FastQCFastQC Report
Fri 10 Feb 2017
ERR1633506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572121
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29090.5084588749582692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.37963997126481985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11020.1926165968387806No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6190.1081938960464657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.10801910784606754No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6040.10557207304049318No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5760.10067800342934449No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5750.10050321522894633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTCC251.2328508E-436.99999631
AACGCCC303.5972393E-430.83333212
CGGAAAC250.00549406529.59999829
GTCGCCA803.274181E-1127.7512
GATCCGT358.86393E-426.4285729
CGCCCCT358.86393E-426.4285714
GGTATCA4500.025.91
TAGTACC751.3706085E-824.6666664
CGAGTCG400.001930008123.12521
CCGTCCT400.001930008123.12532
ATCCGTC400.001930008123.12530
CGTCCTA400.001930008123.12533
TAACGGC652.6789385E-622.76922836
GCTCAAG652.6789385E-622.7692281
TCGCCAT1005.329639E-1022.19999913
CCGATTA502.6998043E-422.19999918
CGTCTGA502.6998043E-422.19999924
GTATCAA11500.021.8782621
CGTGACG1059.804353E-1021.14285736
AAACGCC450.003823493620.55555711
Produced by FastQC (version 0.11.5)