##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633506.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572121
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893008646772273	33.0	33.0	33.0	33.0	33.0
2	32.0157169549798	33.0	33.0	33.0	33.0	33.0
3	31.722182894877132	33.0	33.0	33.0	27.0	33.0
4	32.014703183417495	33.0	33.0	33.0	33.0	33.0
5	32.102794688536164	33.0	33.0	33.0	33.0	33.0
6	35.28741647308873	37.0	37.0	37.0	33.0	37.0
7	35.414153649315445	37.0	37.0	37.0	33.0	37.0
8	35.53597927711096	37.0	37.0	37.0	33.0	37.0
9	35.65070500820631	37.0	37.0	37.0	33.0	37.0
10	35.66237386846489	37.0	37.0	37.0	33.0	37.0
11	35.67382424347297	37.0	37.0	37.0	33.0	37.0
12	35.6830111112859	37.0	37.0	37.0	33.0	37.0
13	35.67790205218826	37.0	37.0	37.0	33.0	37.0
14	35.659517829270385	37.0	37.0	37.0	33.0	37.0
15	35.67163414732198	37.0	37.0	37.0	33.0	37.0
16	35.653150295129876	37.0	37.0	37.0	33.0	37.0
17	35.651010887557	37.0	37.0	37.0	33.0	37.0
18	35.63940145528656	37.0	37.0	37.0	33.0	37.0
19	35.63109552000363	37.0	37.0	37.0	33.0	37.0
20	35.636802354746635	37.0	37.0	37.0	33.0	37.0
21	35.63335028778877	37.0	37.0	37.0	33.0	37.0
22	35.546648348863265	37.0	37.0	37.0	33.0	37.0
23	35.58296059749598	37.0	37.0	37.0	33.0	37.0
24	35.56928516869683	37.0	37.0	37.0	33.0	37.0
25	35.587131043957484	37.0	37.0	37.0	33.0	37.0
26	35.476301341849016	37.0	37.0	37.0	33.0	37.0
27	35.50635442502548	37.0	37.0	37.0	33.0	37.0
28	35.51930623067498	37.0	37.0	37.0	33.0	37.0
29	35.53264606612937	37.0	37.0	37.0	33.0	37.0
30	35.536330601393765	37.0	37.0	37.0	33.0	37.0
31	35.52472466488732	37.0	37.0	37.0	33.0	37.0
32	35.51660050933282	37.0	37.0	37.0	33.0	37.0
33	35.50657291027597	37.0	37.0	37.0	33.0	37.0
34	35.480340697160216	37.0	37.0	37.0	33.0	37.0
35	35.42232849344806	37.0	37.0	37.0	33.0	37.0
36	35.456873633374755	37.0	37.0	37.0	33.0	37.0
37	35.45903051976767	37.0	37.0	37.0	33.0	37.0
38	35.44417002696982	37.0	37.0	37.0	33.0	37.0
39	35.333356055799385	37.0	37.0	37.0	33.0	37.0
40	35.13716853602647	37.0	37.0	37.0	33.0	37.0
41	35.29558257781134	37.0	37.0	37.0	33.0	37.0
42	35.35680913652881	37.0	37.0	37.0	33.0	37.0
43	35.0220809933563	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	19.0
16	22.0
17	20.0
18	27.0
19	15.0
20	29.0
21	89.0
22	243.0
23	610.0
24	1269.0
25	2189.0
26	3593.0
27	5415.0
28	7676.0
29	10490.0
30	13737.0
31	17231.0
32	22006.0
33	29473.0
34	42307.0
35	87292.0
36	328367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90449572730244	22.12067027779089	14.306064626189215	23.668769368717456
2	14.940545793634563	22.605532745695402	40.14745132585589	22.306470134814138
3	16.996579394918207	28.263252004383688	31.91912200391176	22.821046596786342
4	12.065804261685901	18.495213425132096	41.106164605039844	28.332817708142162
5	12.603103189709868	38.66559696288023	36.291798413272716	12.439501434137185
6	27.37725061656538	41.542261165033274	18.36884155624422	12.711646662157131
7	24.160798152838296	33.829731822464126	24.995586597939944	17.013883426757626
8	23.182508595209754	37.03849360537369	21.782105533619635	17.99689226579692
9	24.6758989794117	16.155498574602227	21.66464786295207	37.50395458303401
10	13.62456543283676	29.30464010235597	35.47081823600252	21.599976228804746
11	31.654317880308536	24.986148035118443	25.784405746336876	17.575128338236144
12	21.13119427533686	26.958632876611766	31.90688682988389	20.003286018167486
13	26.199877298683322	23.465490691654388	28.20749456845667	22.12713744120562
14	20.770431429715043	22.5964437592747	29.24748436082577	27.38564045018449
15	22.3957869052176	30.22961925886307	26.095528743045616	21.27906509287371
16	21.626893611666066	30.380985840407888	26.30090487851346	21.69121566941259
17	20.990664562216736	29.070423913822424	27.85564592105516	22.08326560290568
18	20.882121089769473	28.8535117571283	29.558082992933315	20.706284160168913
19	22.717921558551428	27.922065437206466	29.694592577444283	19.665420426797827
20	22.66216412262441	27.47251018578238	29.62817306129298	20.237152630300233
21	21.171570349628837	28.594650432338614	28.96205522957556	21.271723988456987
22	20.890336135188186	28.61143009957684	29.138940888378507	21.35929287685647
23	20.874605197152352	28.32495223912424	29.563676215346053	21.236766348377355
24	21.629515434672037	28.646912104257666	28.58730932792189	21.136263133148407
25	21.747147893540003	28.036202132066467	29.203787310726227	21.0128626636673
26	21.44703655345635	28.342780635564857	29.079163323842334	21.131019487136463
27	21.357894571253283	28.344703305769237	28.67977228593252	21.61762983704496
28	20.861670870322886	28.38979866147196	29.48502152516688	21.26350894303827
29	22.102667093149876	28.442235121591413	28.667886688305444	20.787211096953268
30	21.341639268616255	28.537319902608015	29.073395313229195	21.047645515546538
31	21.516951833615614	28.229343093506444	29.206059557331404	21.047645515546538
32	20.701564878758163	28.137579288297403	29.43153633584504	21.72931949709939
33	20.842618956479487	28.106117412225736	29.781287524841773	21.269976106453008
34	21.120182618711773	28.29383993945337	29.14628199279523	21.439695449039625
35	21.358593724054877	28.82100115185424	28.717701325418926	21.10270379867196
36	21.72197839268267	28.632929048225815	28.608808276570862	21.036284282520658
37	21.775987946605703	27.598532478269455	28.757727823310102	21.867751751814737
38	21.819335420304444	28.295063456856155	28.792860251590135	21.092740871249262
39	21.25214771001239	28.279856883421512	29.0443804719631	21.423614934602995
40	21.347407279229394	28.28702319963784	29.185609337884816	21.17996018324795
41	20.997131725631466	28.12551890246993	29.32631383920534	21.55103553269326
42	21.19027268707144	28.273564508207176	29.069724761020833	21.466438043700546
43	21.289727173098	27.87609614050175	29.076716289036757	21.757460397363495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	57.0
2	65.0
3	231.0
4	397.0
5	397.0
6	450.0
7	503.0
8	469.5
9	436.0
10	659.5
11	883.0
12	883.0
13	1359.5
14	1836.0
15	3519.5
16	5203.0
17	6141.5
18	7080.0
19	7080.0
20	7524.0
21	7968.0
22	9630.0
23	11292.0
24	13277.5
25	15263.0
26	15263.0
27	17601.0
28	19939.0
29	23117.5
30	26296.0
31	28222.5
32	30149.0
33	30149.0
34	32615.5
35	35082.0
36	36747.5
37	38413.0
38	40435.5
39	42458.0
40	42458.0
41	43435.0
42	44412.0
43	42584.0
44	40756.0
45	40251.0
46	39746.0
47	39746.0
48	38110.5
49	36475.0
50	36521.0
51	36567.0
52	35842.0
53	35117.0
54	35117.0
55	31055.5
56	26994.0
57	23595.5
58	20197.0
59	17846.0
60	15495.0
61	15495.0
62	13469.0
63	11443.0
64	9508.5
65	7574.0
66	6381.0
67	5188.0
68	5188.0
69	4219.0
70	3250.0
71	2777.0
72	2304.0
73	1859.0
74	1414.0
75	1414.0
76	1086.0
77	758.0
78	603.5
79	449.0
80	352.5
81	256.0
82	256.0
83	214.5
84	173.0
85	150.0
86	127.0
87	102.5
88	78.0
89	78.0
90	50.5
91	23.0
92	16.0
93	9.0
94	6.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	572121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.795300680207966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34990907311494	53.71259287169057
2	6.790520154182294	8.256634290970496
3	1.694016823096599	3.0896478635246525
4	0.7637980341177012	1.8574132457254957
5	0.4604884714719452	1.3997767541453137
6	0.29119493220692816	1.0621970076043783
7	0.2003084841122518	0.8524470164280706
8	0.16147829196615684	0.7853697050727132
9	0.11549443504619349	0.6319367014971673
>10	0.9461264333750179	11.752207154011092
>50	0.1439234525399031	6.218606635611504
>100	0.08011932768231116	8.66077642628521
>500	0.0017480580585231365	0.6252436915309043
>1k	8.740290292615682E-4	1.095150635902431
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2909	0.5084588749582692	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2172	0.37963997126481985	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1102	0.1926165968387806	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	619	0.1081938960464657	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.10801910784606754	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	604	0.10557207304049318	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	576	0.10067800342934449	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	575	0.10050321522894633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.49576400796335E-4	0.0	0.0
2	0.0	0.0	3.49576400796335E-4	0.0	0.0
3	0.0	0.0	3.49576400796335E-4	0.0	0.0
4	0.0	0.0	3.49576400796335E-4	3.49576400796335E-4	0.0
5	0.0	0.0	3.49576400796335E-4	3.49576400796335E-4	0.0
6	0.0	0.0	6.9915280159267E-4	3.49576400796335E-4	0.0
7	0.0	0.0	0.0019226702043798427	5.243646011945025E-4	0.0
8	0.0	0.0	0.00209745840477801	5.243646011945025E-4	0.0
9	0.0	0.0	0.003320975807565183	0.0012235174027871727	0.0
10	0.0	0.0	0.0038453404087596854	0.0012235174027871727	0.0
11	0.0	0.0	0.0059427988135376955	0.0012235174027871727	0.0
12	0.0	0.0	0.006292375214334031	0.0012235174027871727	0.0
13	0.0	0.0	0.006816739815528533	0.00139830560318534	0.0
14	0.0	0.0	0.0071663162163248684	0.00139830560318534	0.0
15	0.0	0.0	0.007865469017917539	0.00139830560318534	0.0
16	0.0	0.0	0.007865469017917539	0.00139830560318534	0.0
17	0.0	0.0	0.007865469017917539	0.0015730938035835077	0.0
18	0.0	0.0	0.00838983361911204	0.0015730938035835077	0.0
19	0.0	0.0	0.00838983361911204	0.0015730938035835077	0.0
20	0.0	0.0	0.00838983361911204	0.001747882003981675	0.0
21	0.0	0.0	0.009438562821501045	0.00209745840477801	0.0
22	0.0	0.0	0.009438562821501045	0.0022722466051761777	0.0
23	0.0	0.0	0.009438562821501045	0.0024470348055743454	0.0
24	0.0	0.0	0.009438562821501045	0.003670552208361518	0.0
25	0.0	0.0	0.009438562821501045	0.004369705009954188	0.0
26	0.0	0.0	0.009438562821501045	0.004894069611148691	0.0
27	0.0	0.0	0.009438562821501045	0.006467163414732198	0.0
28	0.0	0.0	0.009438562821501045	0.015905726236233243	0.0
29	0.0	0.0	0.009438562821501045	0.03513242828003167	0.0
30	0.0	0.0	0.009438562821501045	0.0613506583397568	0.0
31	0.0	0.0	0.009438562821501045	0.11413669486000338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTCC	25	1.2328508E-4	36.999996	31
AACGCCC	30	3.5972393E-4	30.833332	12
CGGAAAC	25	0.005494065	29.599998	29
GTCGCCA	80	3.274181E-11	27.75	12
GATCCGT	35	8.86393E-4	26.42857	29
CGCCCCT	35	8.86393E-4	26.42857	14
GGTATCA	450	0.0	25.9	1
TAGTACC	75	1.3706085E-8	24.666666	4
CGAGTCG	40	0.0019300081	23.125	21
CCGTCCT	40	0.0019300081	23.125	32
ATCCGTC	40	0.0019300081	23.125	30
CGTCCTA	40	0.0019300081	23.125	33
TAACGGC	65	2.6789385E-6	22.769228	36
GCTCAAG	65	2.6789385E-6	22.769228	1
TCGCCAT	100	5.329639E-10	22.199999	13
CCGATTA	50	2.6998043E-4	22.199999	18
CGTCTGA	50	2.6998043E-4	22.199999	24
GTATCAA	1150	0.0	21.878262	1
CGTGACG	105	9.804353E-10	21.142857	36
AAACGCC	45	0.0038234936	20.555557	11
>>END_MODULE