FastQCFastQC Report
Fri 10 Feb 2017
ERR1633505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314278
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18940.6026511559829196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.4645568573046793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7990.2542335130044101No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6520.20745963764565128No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5730.18232265701067207No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4920.15654929711911109No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4470.14223076384602168No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4290.1365033505367859No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4280.13618516090849503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.13554878165191328No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3850.12250300689198734No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG3830.1218666276354056No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3750.11932111060907859No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3680.11709378321104245No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3560.11327550767155194No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3490.1110481802735158No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3360.10691171510573443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTC352.3828292E-531.71428527
AATGATC250.005490343629.5999987
ACGGACA250.005490343629.5999984
TTGCGGT250.005490343629.59999835
CGGGTAA405.926611E-527.75000223
CCGCTCT405.926611E-527.75000228
CGCTCTC405.926611E-527.75000229
GGTATCA2350.027.5531921
TGCGGGT451.3202253E-424.66666621
TTCGCCG451.3202253E-424.66666624
CCTAGTC400.001928082723.12500227
TCAGACT400.001928082723.1250024
CGATGTC400.001928082723.1250029
GTATCAA7100.022.9295771
GAGTAAT753.7228892E-722.21
GCTTCGC502.6960528E-422.19999922
GCTCGCC502.6960528E-422.19999911
CGTCAAT502.6960528E-422.19999930
AGTACGA502.6960528E-422.1999995
CATCAGA1850.022.016