##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633505.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314278
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.627765863343917	33.0	33.0	33.0	27.0	33.0
2	31.794373134613306	33.0	33.0	33.0	27.0	33.0
3	31.523514213530696	33.0	33.0	33.0	27.0	33.0
4	31.81978057643233	33.0	33.0	33.0	27.0	33.0
5	31.93013192141989	33.0	33.0	33.0	33.0	33.0
6	35.0158426615926	37.0	37.0	37.0	33.0	37.0
7	35.15851889091823	37.0	37.0	37.0	33.0	37.0
8	35.2878025187891	37.0	37.0	37.0	33.0	37.0
9	35.42001030934396	37.0	37.0	37.0	33.0	37.0
10	35.42208808761669	37.0	37.0	37.0	33.0	37.0
11	35.41555565454789	37.0	37.0	37.0	33.0	37.0
12	35.41023870585914	37.0	37.0	37.0	33.0	37.0
13	35.405055396814284	37.0	37.0	37.0	33.0	37.0
14	35.400228460153116	37.0	37.0	37.0	33.0	37.0
15	35.400327098937886	37.0	37.0	37.0	33.0	37.0
16	35.39944571366752	37.0	37.0	37.0	33.0	37.0
17	35.38934637486557	37.0	37.0	37.0	33.0	37.0
18	35.37275596764648	37.0	37.0	37.0	33.0	37.0
19	35.361282049650306	37.0	37.0	37.0	33.0	37.0
20	35.36203615906936	37.0	37.0	37.0	33.0	37.0
21	35.35599055613183	37.0	37.0	37.0	33.0	37.0
22	35.255003531904876	37.0	37.0	37.0	33.0	37.0
23	35.31682777668179	37.0	37.0	37.0	33.0	37.0
24	35.29159533915833	37.0	37.0	37.0	33.0	37.0
25	35.30808710759264	37.0	37.0	37.0	33.0	37.0
26	35.202276328600796	37.0	37.0	37.0	33.0	37.0
27	35.19750348417643	37.0	37.0	37.0	33.0	37.0
28	35.233309363048	37.0	37.0	37.0	33.0	37.0
29	35.246316955052535	37.0	37.0	37.0	33.0	37.0
30	35.23829539452332	37.0	37.0	37.0	33.0	37.0
31	35.21592029986191	37.0	37.0	37.0	33.0	37.0
32	35.20479002666429	37.0	37.0	37.0	33.0	37.0
33	35.192281992376174	37.0	37.0	37.0	33.0	37.0
34	35.170530549386214	37.0	37.0	37.0	33.0	37.0
35	35.102113415511106	37.0	37.0	37.0	33.0	37.0
36	35.1267635660148	37.0	37.0	37.0	33.0	37.0
37	35.1202661338051	37.0	37.0	37.0	33.0	37.0
38	35.106876714246624	37.0	37.0	37.0	33.0	37.0
39	35.002456423930404	37.0	37.0	37.0	27.0	37.0
40	34.78204328651703	37.0	37.0	37.0	27.0	37.0
41	34.96157542048759	37.0	37.0	37.0	27.0	37.0
42	34.98707195540254	37.0	37.0	37.0	27.0	37.0
43	34.63644607640369	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	9.0
17	14.0
18	12.0
19	16.0
20	32.0
21	103.0
22	219.0
23	518.0
24	960.0
25	1660.0
26	2614.0
27	3789.0
28	5410.0
29	6895.0
30	8947.0
31	11177.0
32	14215.0
33	18633.0
34	26367.0
35	52013.0
36	160661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.868594047308434	21.008788397533394	13.643653071484483	24.47896448367369
2	16.311991294331772	21.68653230579296	38.71349569489433	23.28798070498094
3	17.406881805280676	27.8788206619617	30.00210005154672	24.7121974812109
4	12.292938099389712	18.045488389260463	40.13643971261113	29.525133798738697
5	13.404692660638034	37.83561051043981	35.48005269220245	13.279644136719721
6	28.937119365657153	40.570132175971594	17.32860715672112	13.164141301650131
7	26.079140124348505	32.59375457397591	23.77831092217718	17.548794379498407
8	24.36568897600214	35.86347119429295	20.64764316942325	19.12319666028166
9	25.860543849712673	15.124189411921929	20.511139818886466	38.504126919478935
10	15.249237935840243	28.112371849127204	33.855694639777525	22.78269557525503
11	33.52064096118723	24.228549246208768	24.036362710721082	18.21444708188292
12	23.108521754624885	26.25159890288216	29.904415835661418	20.73546350683153
13	27.09925607264906	22.712057477774454	26.622926199097613	23.565760250478878
14	21.833854103691635	21.003379173852448	28.36883268952965	28.79393403292626
15	23.823812039022776	28.78884299887361	25.33807648005906	22.04926848204456
16	22.596236453076575	28.6217934440209	25.385168545046106	23.39680155785642
17	21.898764787862977	27.82281928738251	26.430103284353347	23.848312640401172
18	22.298410960996314	27.432082423841315	28.39556061830609	21.873945996856285
19	24.512374394644233	26.16536951361533	27.9835050496694	21.33875104207103
20	23.484621895264702	26.481968193764754	28.37837837837838	21.655031532592165
21	22.69201153119213	27.411081908374115	28.23360209750603	21.663304462927726
22	22.49887042681957	27.11198365778069	27.933549278027737	22.455596637372008
23	22.471824308414842	27.084301160119384	28.623066202534066	21.820808328931708
24	22.777604541202376	27.45212837042364	26.92616091485882	22.84410617351517
25	22.604191193783848	27.111665468152403	28.543200605833054	21.7409427322307
26	22.812923589942663	27.287306142968966	28.524745607392184	21.375024659696194
27	22.92365358058789	27.142211672468324	27.12980227696498	22.804332469978807
28	22.07122356639663	26.79252127097665	28.864253940778546	22.27200122184817
29	23.794856782848306	26.49660491666614	27.762363258007245	21.946175042478316
30	22.92524452872934	26.294554502701427	28.513608970402	22.26659199816723
31	22.49537034090837	26.82497661306232	28.32078605565773	22.35886699037158
32	21.593939123960315	26.73047429345993	28.55688275984956	23.118703822730193
33	21.854854619158832	26.989162461260413	28.729341538383213	22.426641381197538
34	22.655419723938678	26.931888328168057	28.561019225017343	21.851672722875925
35	22.682147652715113	27.321034243567798	27.96377729271537	22.033040811001726
36	23.36752811205366	27.091619521570077	27.227168303221987	22.313684063154277
37	22.501734133474187	26.6095622347094	27.85495643983989	23.03374719197653
38	23.070338999229982	26.99138978865845	27.650678698477144	22.287592513634426
39	21.840217896257453	27.263760110475438	28.134963312735856	22.76105868053125
40	22.37191276513151	27.53803957006217	28.33192269264791	21.758124972158406
41	21.844672551053527	26.50742336402803	28.527927503675087	23.119976581243357
42	21.690350581332453	27.709543779710955	28.13241779570953	22.46768784324706
43	22.779513678972123	25.928000050910338	28.33542277855911	22.95706349155843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	36.0
2	46.0
3	158.5
4	271.0
5	271.0
6	292.0
7	313.0
8	280.5
9	248.0
10	352.5
11	457.0
12	457.0
13	737.0
14	1017.0
15	1848.0
16	2679.0
17	2960.0
18	3241.0
19	3241.0
20	3036.0
21	2831.0
22	3047.5
23	3264.0
24	4036.5
25	4809.0
26	4809.0
27	5901.0
28	6993.0
29	8128.0
30	9263.0
31	10744.0
32	12225.0
33	12225.0
34	14910.0
35	17595.0
36	19061.0
37	20527.0
38	21766.5
39	23006.0
40	23006.0
41	23877.0
42	24748.0
43	25285.0
44	25822.0
45	24731.5
46	23641.0
47	23641.0
48	22361.0
49	21081.0
50	21302.0
51	21523.0
52	20459.0
53	19395.0
54	19395.0
55	19190.0
56	18985.0
57	16409.0
58	13833.0
59	12234.0
60	10635.0
61	10635.0
62	9731.0
63	8827.0
64	6929.5
65	5032.0
66	4126.5
67	3221.0
68	3221.0
69	2737.0
70	2253.0
71	2009.0
72	1765.0
73	1917.0
74	2069.0
75	2069.0
76	1824.0
77	1579.0
78	1134.5
79	690.0
80	416.0
81	142.0
82	142.0
83	127.5
84	113.0
85	84.0
86	55.0
87	44.0
88	33.0
89	33.0
90	26.5
91	20.0
92	11.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	314278.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.55622147604762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09069362305854	49.82076778524354
2	6.154874718440957	6.961929154669459
3	1.6814126641331029	2.8528304107602906
4	0.8994230553988604	2.0347187808720557
5	0.5729951731368516	1.620322095831701
6	0.36154319097891086	1.2268510069296155
7	0.26043261568629616	1.031035928463839
8	0.24145998504593438	1.092485151348881
9	0.1755907976619449	0.8937676837552336
>10	1.2899441158959148	14.511931245107007
>50	0.17880888682744905	7.004210059083982
>100	0.08997389846731514	9.217099856581042
>500	0.0017083651607718061	0.651854016902059
>1k	0.0011389101071812042	1.080196824451337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.6026511559829196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1460	0.4645568573046793	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	799	0.2542335130044101	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	652	0.20745963764565128	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	573	0.18232265701067207	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	492	0.15654929711911109	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	447	0.14223076384602168	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	429	0.1365033505367859	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	428	0.13618516090849503	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.13554878165191328	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	385	0.12250300689198734	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	383	0.1218666276354056	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	375	0.11932111060907859	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	368	0.11709378321104245	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	356	0.11327550767155194	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	349	0.1110481802735158	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	336	0.10691171510573443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	3.181896282908762E-4	0.0	0.0
6	0.0	0.0	6.363792565817524E-4	0.0	0.0
7	0.0	0.0	0.0035000859111996387	6.363792565817524E-4	0.0
8	0.0	0.0	0.0035000859111996387	6.363792565817524E-4	0.0
9	0.0	0.0	0.0035000859111996387	0.0012727585131635049	0.0
10	0.0	0.0	0.0038182755394905146	0.0015909481414543812	0.0
11	0.0	0.0	0.004772844424363144	0.0019091377697452573	0.0
12	0.0	0.0	0.005409223680944896	0.0019091377697452573	0.0
13	0.0	0.0	0.005409223680944896	0.0019091377697452573	0.0
14	0.0	0.0	0.005409223680944896	0.0019091377697452573	0.0
15	0.0	0.0	0.005409223680944896	0.0019091377697452573	0.0
16	0.0	0.0	0.006045602937526648	0.0022273273980361334	0.0
17	0.0	0.0	0.006363792565817525	0.0025455170263270098	0.0
18	0.0	0.0	0.006363792565817525	0.0031818962829087624	0.0
19	0.0	0.0	0.006363792565817525	0.0031818962829087624	0.0
20	0.0	0.0	0.006681982194108401	0.0035000859111996387	0.0
21	0.0	0.0	0.007318361450690153	0.0035000859111996387	3.181896282908762E-4
22	0.0	0.0	0.007318361450690153	0.004772844424363144	3.181896282908762E-4
23	0.0	0.0	0.007318361450690153	0.005409223680944896	3.181896282908762E-4
24	0.0	0.0	0.007318361450690153	0.006045602937526648	3.181896282908762E-4
25	0.0	0.0	0.007318361450690153	0.006363792565817525	3.181896282908762E-4
26	0.0	0.0	0.007636551078981029	0.007636551078981029	3.181896282908762E-4
27	0.0	0.0	0.007636551078981029	0.009545688848726287	3.181896282908762E-4
28	0.0	0.0	0.007636551078981029	0.021636894723779585	3.181896282908762E-4
29	0.0	0.0	0.007636551078981029	0.04581930647388618	3.181896282908762E-4
30	0.0	0.0	0.007636551078981029	0.08145654484246431	3.181896282908762E-4
31	0.0	0.0	0.007636551078981029	0.13077593722755013	3.181896282908762E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCTC	35	2.3828292E-5	31.714285	27
AATGATC	25	0.0054903436	29.599998	7
ACGGACA	25	0.0054903436	29.599998	4
TTGCGGT	25	0.0054903436	29.599998	35
CGGGTAA	40	5.926611E-5	27.750002	23
CCGCTCT	40	5.926611E-5	27.750002	28
CGCTCTC	40	5.926611E-5	27.750002	29
GGTATCA	235	0.0	27.553192	1
TGCGGGT	45	1.3202253E-4	24.666666	21
TTCGCCG	45	1.3202253E-4	24.666666	24
CCTAGTC	40	0.0019280827	23.125002	27
TCAGACT	40	0.0019280827	23.125002	4
CGATGTC	40	0.0019280827	23.125002	9
GTATCAA	710	0.0	22.929577	1
GAGTAAT	75	3.7228892E-7	22.2	1
GCTTCGC	50	2.6960528E-4	22.199999	22
GCTCGCC	50	2.6960528E-4	22.199999	11
CGTCAAT	50	2.6960528E-4	22.199999	30
AGTACGA	50	2.6960528E-4	22.199999	5
CATCAGA	185	0.0	22.0	16
>>END_MODULE