FastQCFastQC Report
Fri 10 Feb 2017
ERR1633504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373463
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21330.5711409162353432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.4359200242058785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8330.2230475308129587No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7630.20430404082867645No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7520.20135863525971784No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7400.19814546554812656No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6370.17056575885696842No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5620.15048344815952316No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5300.14191499559527984No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5210.13950511831158643No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5120.137095241027893No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5030.13468536374419957No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4720.12638467532258885No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4720.12638467532258885No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4540.12156492075520199No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4470.11969057175677378No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4360.11674516618781511No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT4290.1148708171893869No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.11379976061885648No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4170.11165764747779565No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4100.10978329847936744No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4100.10978329847936744No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4010.10737342119567399No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC3920.10496354391198058No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG3800.10175037420038933No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT3790.10148261005775673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTTA551.848639E-830.27272829
GGTATCA3250.030.169231
CCGCTGC250.005491652529.59999818
ACGGATC250.005491652529.5999988
CCCGGTC250.005491652529.59999813
AACGATT405.9295606E-527.7522
TTGCGGT358.85814E-426.4285735
GTATCAA9550.023.6335071
ACGTGAT652.6748003E-622.7692337
TTCAACG502.6973718E-422.19999919
TACGTGA502.6973718E-422.19999936
TATACTG705.0867184E-621.1428575
TGGACCT450.003821046620.55555523
AGACGAA450.003821046620.55555515
TTAAGAT450.003821046620.5555553
GTTTAGG1001.2827513E-820.3499981
CCTACGT555.1341625E-420.18181834
TGCGGTA555.1341625E-420.18181836
CTACGTG555.1341625E-420.18181835
CCGAGTT555.1341625E-420.18181813
Produced by FastQC (version 0.11.5)