##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633504.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373463
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873968773345688	33.0	33.0	33.0	33.0	33.0
2	31.978321815012464	33.0	33.0	33.0	33.0	33.0
3	31.696982030348387	33.0	33.0	33.0	27.0	33.0
4	31.983701196637952	33.0	33.0	33.0	33.0	33.0
5	32.08326929307589	33.0	33.0	33.0	33.0	33.0
6	35.25749806540407	37.0	37.0	37.0	33.0	37.0
7	35.40799490177072	37.0	37.0	37.0	33.0	37.0
8	35.51686512452371	37.0	37.0	37.0	33.0	37.0
9	35.62947601234928	37.0	37.0	37.0	33.0	37.0
10	35.63531327065867	37.0	37.0	37.0	33.0	37.0
11	35.66153005786383	37.0	37.0	37.0	33.0	37.0
12	35.65514120542062	37.0	37.0	37.0	33.0	37.0
13	35.65213421409885	37.0	37.0	37.0	33.0	37.0
14	35.62469642240329	37.0	37.0	37.0	33.0	37.0
15	35.634472491250804	37.0	37.0	37.0	33.0	37.0
16	35.62631104018337	37.0	37.0	37.0	33.0	37.0
17	35.64007411711468	37.0	37.0	37.0	33.0	37.0
18	35.619118895312255	37.0	37.0	37.0	33.0	37.0
19	35.61184642119835	37.0	37.0	37.0	33.0	37.0
20	35.612076698361015	37.0	37.0	37.0	33.0	37.0
21	35.6151265319456	37.0	37.0	37.0	33.0	37.0
22	35.513614467832156	37.0	37.0	37.0	33.0	37.0
23	35.55304006019338	37.0	37.0	37.0	33.0	37.0
24	35.55042400451986	37.0	37.0	37.0	33.0	37.0
25	35.57140600273655	37.0	37.0	37.0	33.0	37.0
26	35.45707606911528	37.0	37.0	37.0	33.0	37.0
27	35.47663891737602	37.0	37.0	37.0	33.0	37.0
28	35.4963490359152	37.0	37.0	37.0	33.0	37.0
29	35.511011800365765	37.0	37.0	37.0	33.0	37.0
30	35.502745921282695	37.0	37.0	37.0	33.0	37.0
31	35.47700843189285	37.0	37.0	37.0	33.0	37.0
32	35.48345083716459	37.0	37.0	37.0	33.0	37.0
33	35.4716424384745	37.0	37.0	37.0	33.0	37.0
34	35.46646923523883	37.0	37.0	37.0	33.0	37.0
35	35.38443433486048	37.0	37.0	37.0	33.0	37.0
36	35.4200041235678	37.0	37.0	37.0	33.0	37.0
37	35.41739610081856	37.0	37.0	37.0	33.0	37.0
38	35.394997630287335	37.0	37.0	37.0	33.0	37.0
39	35.30680950991129	37.0	37.0	37.0	33.0	37.0
40	35.09429849811092	37.0	37.0	37.0	33.0	37.0
41	35.25505337878183	37.0	37.0	37.0	33.0	37.0
42	35.29268495138742	37.0	37.0	37.0	33.0	37.0
43	34.94403461654836	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	10.0
16	13.0
17	15.0
18	13.0
19	11.0
20	27.0
21	56.0
22	169.0
23	344.0
24	795.0
25	1479.0
26	2415.0
27	3550.0
28	5123.0
29	6936.0
30	8922.0
31	11459.0
32	15054.0
33	19876.0
34	28388.0
35	58710.0
36	210095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.907666890696	21.049207016491593	13.675250292532326	24.367875800280082
2	16.150997555313378	21.472274361851106	38.940939263059526	23.435788819775986
3	17.33290848089369	27.941188283712176	29.81848268770936	24.90742054768478
4	12.146852566385427	17.87004335101469	40.46853369677853	29.514570385821354
5	13.190329430224681	37.92022235134404	35.57005647145768	13.319391746973597
6	28.73457343833258	40.76387754610229	17.407614676688183	13.093934338876945
7	26.11905329309731	32.49933728374698	23.912408993662023	17.469200429493682
8	24.14697038260818	35.89619319718419	20.672998396092783	19.28383802411484
9	25.767211209678067	15.254255441636788	20.485563496249963	38.49296985243518
10	15.174729491274904	28.09113620358643	33.80816841293515	22.92596589220351
11	33.39607939742357	24.297453830767708	24.071193130243156	18.235273641565563
12	23.128395584033758	26.255345241697302	29.650326806136086	20.965932368132854
13	27.098802290990005	22.877232818244376	26.391369426154665	23.632595464610954
14	21.696392949234596	21.13194613656507	28.33855027137896	28.833110642821374
15	23.83689950543963	28.86979433036204	25.296481846929954	21.996824317268377
16	22.586976487630636	28.44271052286304	25.29862396007101	23.67168902943531
17	21.798143323434985	28.044545242768358	26.44626107539435	23.711050358402304
18	22.122941228448333	27.571673766879183	28.472967870980526	21.83241713369196
19	24.41205688381446	26.22321354458139	27.865411031347147	21.499318540257
20	23.437395404631783	26.47946382908079	28.46359612598838	21.619544640299036
21	22.83733596099212	27.261870653853258	28.161022644813542	21.739770740341076
22	22.260036469476226	27.409944224729088	27.71332099833183	22.616698307462855
23	22.158286095275837	27.230542249165246	28.545264189491327	22.06590746606759
24	22.634102976733974	27.881744644047735	26.578268797712223	22.905883581506068
25	22.488439283141837	27.3328281516509	28.29115601813299	21.887576547074275
26	22.843226772130038	27.453589779978206	28.212433360199	21.490750087692756
27	23.053421624096632	27.14753536494914	27.118349073402186	22.680693937552046
28	21.827597379124573	27.11701025268902	28.795891426995446	22.25950094119096
29	24.014159367862412	26.68751656790633	27.45225095926504	21.84607310496622
30	23.087695434353602	26.32523168292441	28.434677598584063	22.15239528413792
31	22.54440198895205	26.90681540072243	28.072928241887418	22.475854368438107
32	21.42300575960671	26.615488013538158	28.587035395742017	23.374470831113122
33	21.70362258108568	26.95929717267842	28.77072159758798	22.566358648647924
34	22.62151806203024	27.038823123040302	28.540712199066576	21.79894661586288
35	22.737995464075425	27.51571106106897	27.698593970487035	22.047699504368573
36	23.669011388008986	27.202962542474086	27.043642877607688	22.084383191909236
37	22.524051914111972	26.424304415698472	27.74946915758723	23.30217451260232
38	22.98889046572217	27.012046708777042	27.518656466637925	22.480406358862858
39	22.10580432331985	27.15289064780179	28.12755212698447	22.613752901893896
40	22.19978953738389	27.79284694869371	28.15352524881983	21.853838265102567
41	22.104733266749317	26.46259468809494	28.279909924142416	23.152762121013325
42	21.692376487095107	27.86889196520137	27.847470833790766	22.591260713912757
43	22.813504952297816	26.05639648372128	28.01214578150982	23.11795278247109
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	44.5
2	46.0
3	153.0
4	260.0
5	260.0
6	318.5
7	377.0
8	318.5
9	260.0
10	369.5
11	479.0
12	479.0
13	796.5
14	1114.0
15	2031.0
16	2948.0
17	3290.5
18	3633.0
19	3633.0
20	3402.5
21	3172.0
22	3380.0
23	3588.0
24	4451.5
25	5315.0
26	5315.0
27	6642.5
28	7970.0
29	9240.5
30	10511.0
31	12415.5
32	14320.0
33	14320.0
34	17587.0
35	20854.0
36	22694.5
37	24535.0
38	26255.5
39	27976.0
40	27976.0
41	29160.0
42	30344.0
43	31049.5
44	31755.0
45	30366.0
46	28977.0
47	28977.0
48	27321.5
49	25666.0
50	25695.0
51	25724.0
52	24510.0
53	23296.0
54	23296.0
55	23160.5
56	23025.0
57	19539.5
58	16054.0
59	14244.5
60	12435.0
61	12435.0
62	11181.5
63	9928.0
64	7910.0
65	5892.0
66	4740.5
67	3589.0
68	3589.0
69	3011.0
70	2433.0
71	2170.0
72	1907.0
73	2053.5
74	2200.0
75	2200.0
76	1961.5
77	1723.0
78	1213.5
79	704.0
80	434.0
81	164.0
82	164.0
83	142.0
84	120.0
85	95.5
86	71.0
87	54.0
88	37.0
89	37.0
90	25.5
91	14.0
92	8.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	373463.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.327566592999574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.12817213457748	45.592052294984335
2	6.509818226862198	6.812858935489081
3	1.8548434446854636	2.91178331614102
4	0.9323915255680498	1.9515911857964239
5	0.589316495327394	1.541874907679867
6	0.4242357198431049	1.3319533728731512
7	0.29891298049544646	1.094897222467122
8	0.2528397311161274	1.058439029386821
9	0.2051577951289594	0.966186736801523
>10	1.4868456864875927	15.45073462806054
>50	0.18683670050338821	6.7818776136792795
>100	0.12438432150504111	11.75138741950265
>500	0.0052043649165289165	1.7301236188292355
>1k	0.0010408729833057833	1.0242397183089493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2133	0.5711409162353432	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1628	0.4359200242058785	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	833	0.2230475308129587	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	763	0.20430404082867645	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	752	0.20135863525971784	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	740	0.19814546554812656	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	637	0.17056575885696842	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	562	0.15048344815952316	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	530	0.14191499559527984	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	521	0.13950511831158643	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	512	0.137095241027893	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	503	0.13468536374419957	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	472	0.12638467532258885	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	472	0.12638467532258885	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	454	0.12156492075520199	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	447	0.11969057175677378	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	436	0.11674516618781511	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	429	0.1148708171893869	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.11379976061885648	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	417	0.11165764747779565	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	410	0.10978329847936744	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	410	0.10978329847936744	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	401	0.10737342119567399	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	392	0.10496354391198058	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	380	0.10175037420038933	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	379	0.10148261005775673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.677641426326035E-4	0.0
3	0.0	0.0	2.677641426326035E-4	2.677641426326035E-4	0.0
4	0.0	0.0	2.677641426326035E-4	2.677641426326035E-4	0.0
5	0.0	0.0	2.677641426326035E-4	2.677641426326035E-4	0.0
6	0.0	0.0	5.35528285265207E-4	2.677641426326035E-4	0.0
7	0.0	0.0	0.0016065848557956209	2.677641426326035E-4	0.0
8	0.0	0.0	0.0018743489984282245	2.677641426326035E-4	0.0
9	0.0	0.0	0.002142113141060828	5.35528285265207E-4	0.0
10	0.0	0.0	0.0024098772836934315	5.35528285265207E-4	0.0
11	0.0	0.0	0.004016462139489052	8.032924278978104E-4	0.0
12	0.0	0.0	0.004551990424754259	8.032924278978104E-4	0.0
13	0.0	0.0	0.00535528285265207	8.032924278978104E-4	0.0
14	0.0	0.0	0.005623046995284674	0.001071056570530414	0.0
15	2.677641426326035E-4	0.0	0.006961867708447691	0.0013388207131630175	0.0
16	2.677641426326035E-4	0.0	0.007497395993712898	0.0018743489984282245	0.0
17	2.677641426326035E-4	0.0	0.007765160136345502	0.002142113141060828	0.0
18	2.677641426326035E-4	0.0	0.008032924278978105	0.0024098772836934315	0.0
19	2.677641426326035E-4	0.0	0.008300688421610709	0.0024098772836934315	0.0
20	2.677641426326035E-4	0.0	0.008568452564243313	0.002677641426326035	0.0
21	2.677641426326035E-4	0.0	0.008836216706875915	0.0029454055689586383	0.0
22	5.35528285265207E-4	0.0	0.009103980849508518	0.004551990424754259	0.0
23	5.35528285265207E-4	0.0	0.009103980849508518	0.004551990424754259	0.0
24	5.35528285265207E-4	0.0	0.009103980849508518	0.005623046995284674	0.0
25	5.35528285265207E-4	0.0	0.009103980849508518	0.005623046995284674	0.0
26	5.35528285265207E-4	0.0	0.009103980849508518	0.0061585752805498805	0.0
27	5.35528285265207E-4	0.0	0.009103980849508518	0.008568452564243313	0.0
28	5.35528285265207E-4	0.0	0.009103980849508518	0.024098772836934314	0.0
29	5.35528285265207E-4	0.0	0.009103980849508518	0.04364555524911437	0.0
30	5.35528285265207E-4	0.0	0.009103980849508518	0.07738383722082241	0.0
31	5.35528285265207E-4	0.0	0.009103980849508518	0.1365597127426278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCTTA	55	1.848639E-8	30.272728	29
GGTATCA	325	0.0	30.16923	1
CCGCTGC	25	0.0054916525	29.599998	18
ACGGATC	25	0.0054916525	29.599998	8
CCCGGTC	25	0.0054916525	29.599998	13
AACGATT	40	5.9295606E-5	27.75	22
TTGCGGT	35	8.85814E-4	26.42857	35
GTATCAA	955	0.0	23.633507	1
ACGTGAT	65	2.6748003E-6	22.76923	37
TTCAACG	50	2.6973718E-4	22.199999	19
TACGTGA	50	2.6973718E-4	22.199999	36
TATACTG	70	5.0867184E-6	21.142857	5
TGGACCT	45	0.0038210466	20.555555	23
AGACGAA	45	0.0038210466	20.555555	15
TTAAGAT	45	0.0038210466	20.555555	3
GTTTAGG	100	1.2827513E-8	20.349998	1
CCTACGT	55	5.1341625E-4	20.181818	34
TGCGGTA	55	5.1341625E-4	20.181818	36
CTACGTG	55	5.1341625E-4	20.181818	35
CCGAGTT	55	5.1341625E-4	20.181818	13
>>END_MODULE