FastQCFastQC Report
Fri 10 Feb 2017
ERR1633503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624477
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42860.6863343245627942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.5274813964325348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16560.26518190421744914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.14860435212185558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.028.0925941
GGTAACG659.377436E-825.61538525
TGCGGGT701.9199979E-723.78571521
GTATCAA16850.023.6053411
TCGCCGT400.001930204623.12500225
CACGATA400.001930204623.12500216
CGCCGTC400.001930204623.12500226
GCGGGTA652.6795879E-622.7692322
GTATTGG753.7351128E-722.21
CGTGGGA1059.804353E-1021.14285737
ACGTGGG1059.804353E-1021.14285736
TGCTAGC1059.804353E-1021.14285728
CGAGTCG450.003823879620.55555721
GGCGTCA450.003823879620.55555725
CCGTCCG450.003823879620.55555728
ACCCGTC450.003823879620.55555725
TGTCCGG555.13948E-420.18181814
TACCCTC1750.020.0857145
CGGGTAA759.25466E-619.73333423
CAACCCA2100.019.3809517
Produced by FastQC (version 0.11.5)