##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633503.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	624477
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89386798873297	33.0	33.0	33.0	33.0	33.0
2	32.01671798961371	33.0	33.0	33.0	33.0	33.0
3	31.72985714445848	33.0	33.0	33.0	27.0	33.0
4	32.01290039505058	33.0	33.0	33.0	33.0	33.0
5	32.10310708000455	33.0	33.0	33.0	33.0	33.0
6	35.28892657375692	37.0	37.0	37.0	33.0	37.0
7	35.42304360288689	37.0	37.0	37.0	33.0	37.0
8	35.55015957353113	37.0	37.0	37.0	33.0	37.0
9	35.65547650273749	37.0	37.0	37.0	33.0	37.0
10	35.658105903019646	37.0	37.0	37.0	33.0	37.0
11	35.67519540351366	37.0	37.0	37.0	33.0	37.0
12	35.68101947709844	37.0	37.0	37.0	33.0	37.0
13	35.68135896117872	37.0	37.0	37.0	33.0	37.0
14	35.65226901871486	37.0	37.0	37.0	33.0	37.0
15	35.66224216424304	37.0	37.0	37.0	33.0	37.0
16	35.64800465029136	37.0	37.0	37.0	33.0	37.0
17	35.6520720538947	37.0	37.0	37.0	33.0	37.0
18	35.6324380241386	37.0	37.0	37.0	33.0	37.0
19	35.63214497891836	37.0	37.0	37.0	33.0	37.0
20	35.63220422849841	37.0	37.0	37.0	33.0	37.0
21	35.634481333980276	37.0	37.0	37.0	33.0	37.0
22	35.52743335623249	37.0	37.0	37.0	33.0	37.0
23	35.58709768334142	37.0	37.0	37.0	33.0	37.0
24	35.57580983767216	37.0	37.0	37.0	33.0	37.0
25	35.59724857760975	37.0	37.0	37.0	33.0	37.0
26	35.48237805395555	37.0	37.0	37.0	33.0	37.0
27	35.505856901054806	37.0	37.0	37.0	33.0	37.0
28	35.51553379868274	37.0	37.0	37.0	33.0	37.0
29	35.52249802634845	37.0	37.0	37.0	33.0	37.0
30	35.526181108351466	37.0	37.0	37.0	33.0	37.0
31	35.51413743020159	37.0	37.0	37.0	33.0	37.0
32	35.5017094304514	37.0	37.0	37.0	33.0	37.0
33	35.491007675222626	37.0	37.0	37.0	33.0	37.0
34	35.46090728721794	37.0	37.0	37.0	33.0	37.0
35	35.40567066521265	37.0	37.0	37.0	33.0	37.0
36	35.42444957940805	37.0	37.0	37.0	33.0	37.0
37	35.4274392811905	37.0	37.0	37.0	33.0	37.0
38	35.39570872906448	37.0	37.0	37.0	33.0	37.0
39	35.29234063063972	37.0	37.0	37.0	33.0	37.0
40	35.08959817575347	37.0	37.0	37.0	33.0	37.0
41	35.24015295999693	37.0	37.0	37.0	33.0	37.0
42	35.274953280905464	37.0	37.0	37.0	33.0	37.0
43	34.95154985692027	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	7.0
15	13.0
16	39.0
17	25.0
18	24.0
19	28.0
20	33.0
21	120.0
22	266.0
23	657.0
24	1299.0
25	2335.0
26	3920.0
27	5915.0
28	8488.0
29	11376.0
30	14911.0
31	19420.0
32	24571.0
33	32366.0
34	46735.0
35	96088.0
36	355841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38419349311504	21.704882645798005	14.447129357846647	23.46379450324031
2	15.320340060562678	22.585299378519945	39.87208496069511	22.222275600222265
3	17.192626790097954	28.18870831111474	31.28778161565598	23.330883283131325
4	11.945676141795454	18.564654903223015	40.910233683546394	28.579435271435138
5	12.82433140051595	37.982183491145385	36.00260698152214	13.190878126816518
6	27.364018210438495	41.40424707395148	18.224530286944116	13.007204428665908
7	24.69618576825087	33.44206431942249	24.517796492104594	17.343953420222043
8	23.711201533443184	36.31935203378187	21.529215647653956	18.44023078512099
9	25.248007532703365	15.78745093894571	21.389578799539454	37.57496272881147
10	14.251125341685922	28.940857709731503	34.80544519654046	22.00257175204211
11	32.104144748325396	24.803955950339244	25.33159748077191	17.76030182056345
12	21.65396003375625	26.659748877860995	31.588993669902976	20.097297418479783
13	27.12541855024284	23.170589148999884	27.497409832547877	22.206582468209398
14	21.297661883464084	22.132280292148472	29.40236389810994	27.167693926277508
15	22.828863192719666	29.620306272288648	25.940266815271016	21.610563719720663
16	21.611204255721187	29.86130794248627	26.277669153547688	22.24981864824485
17	21.088526879292594	28.677437279515498	27.795579340792376	22.438456500399536
18	21.601115813712916	27.99766844895809	29.43759337813883	20.963622359190172
19	23.382606565173738	27.160648030271734	29.559295218238624	19.89745018631591
20	23.065060842913347	26.9385421720896	29.48114982617454	20.515247158822504
21	21.755004587839103	27.763552540766113	29.091383669854938	21.39005920153985
22	21.249141281424297	27.80742925680209	29.217409127958277	21.726020333815338
23	21.27252084544347	27.64008922666487	29.81358801044714	21.27380191744452
24	21.662287001763076	27.82808654281903	28.858708967664143	21.650917487753752
25	21.745716815831486	27.686367952702824	29.487875454180056	21.080039777285634
26	21.754844453838974	27.897264430875758	29.034055697807926	21.313835417477346
27	21.726500735815733	27.894382018873394	28.574791385431332	21.804325859879548
28	21.349545299506627	27.78645170278489	29.44303793414329	21.42096506356519
29	22.166869236176833	27.741454048747993	28.841094227649698	21.25058248742548
30	21.68358482378054	27.78148754878082	29.293472778020647	21.24145484941799
31	21.757246463840943	27.647615524671043	29.178336431926237	21.416801579561778
32	20.85681298110259	27.619592074648065	29.54296155022523	21.980633394024117
33	21.07955937528524	27.512782696560485	29.940894540551533	21.466763387602747
34	21.467884325603663	27.418944172483535	29.34679740006437	21.766374101848427
35	21.43649806157793	28.285909649194448	28.985855363768405	21.291736925459222
36	22.04084377807349	27.703662424717002	28.923403103717188	21.332090693492315
37	22.14717275416068	27.077538484203583	29.0098754637881	21.765413297847637
38	21.892239425951637	27.689410498705318	28.924043639717716	21.494306435625333
39	21.429772433572413	27.546250702587926	29.279861388009486	21.744115475830174
40	21.42320693956703	28.09871300304094	29.18858500793464	21.289495049457386
41	21.332090693492315	27.461699950518593	29.302600416028135	21.903608939960957
42	21.34586221750361	27.872603794855532	29.04542521181725	21.73610877582361
43	21.64355132374771	27.259931110353143	29.123890871881592	21.972626694017556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	82.0
2	97.0
3	335.0
4	573.0
5	573.0
6	665.5
7	758.0
8	690.5
9	623.0
10	898.5
11	1174.0
12	1174.0
13	1849.5
14	2525.0
15	4607.5
16	6690.0
17	7641.0
18	8592.0
19	8592.0
20	8634.0
21	8676.0
22	9816.0
23	10956.0
24	12845.0
25	14734.0
26	14734.0
27	17146.0
28	19558.0
29	22577.5
30	25597.0
31	27793.5
32	29990.0
33	29990.0
34	32978.5
35	35967.0
36	37839.0
37	39711.0
38	41901.5
39	44092.0
40	44092.0
41	45064.0
42	46036.0
43	45390.5
44	44745.0
45	44645.5
46	44546.0
47	44546.0
48	42888.5
49	41231.0
50	41319.5
51	41408.0
52	40550.5
53	39693.0
54	39693.0
55	36132.5
56	32572.0
57	28986.5
58	25401.0
59	22312.0
60	19223.0
61	19223.0
62	16692.0
63	14161.0
64	11590.5
65	9020.0
66	7577.0
67	6134.0
68	6134.0
69	4967.5
70	3801.0
71	3290.5
72	2780.0
73	2166.0
74	1552.0
75	1552.0
76	1147.5
77	743.0
78	571.0
79	399.0
80	320.5
81	242.0
82	242.0
83	203.0
84	164.0
85	143.5
86	123.0
87	104.0
88	85.0
89	85.0
90	57.5
91	30.0
92	19.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	624477.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.85575472005219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.46409352577189	52.35229328890306
2	7.227506433443705	8.652157046356113
3	1.7528990804659286	3.1476329222812094
4	0.8718807699687516	2.087483260495194
5	0.46808799602624945	1.4008880138773974
6	0.318078085820356	1.1423282332012163
7	0.23720957587390337	0.9938850733529179
8	0.17882334394690677	0.8562884970804466
9	0.13735736820485508	0.7399466046235531
>10	1.137078817690361	13.912801312575116
>50	0.13618149395658938	5.646442565825552
>100	0.06917584196087306	7.24324101919888
>500	8.138334348336383E-4	0.3249131384373935
>1k	8.138334348336383E-4	1.499699023791987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4286	0.6863343245627942	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3294	0.5274813964325348	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1656	0.26518190421744914	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	928	0.14860435212185558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.6013400013130988E-4	0.0	0.0
4	0.0	0.0	1.6013400013130988E-4	0.0	0.0
5	0.0	0.0	1.6013400013130988E-4	0.0	0.0
6	0.0	0.0	0.0014412060011817888	0.0	0.0
7	0.0	0.0	0.002562144002100958	0.0	0.0
8	0.0	0.0	0.0032026800026261977	0.0	0.0
9	0.0	0.0	0.003843216003151437	6.405360005252395E-4	0.0
10	3.2026800026261975E-4	0.0	0.004804020003939297	6.405360005252395E-4	0.0
11	3.2026800026261975E-4	0.0	0.007686432006302874	8.006700006565494E-4	0.0
12	3.2026800026261975E-4	0.0	0.008166834006696804	8.006700006565494E-4	0.0
13	3.2026800026261975E-4	0.0	0.009447906007747283	9.608040007878593E-4	0.0
14	3.2026800026261975E-4	0.0	0.010088442008272523	9.608040007878593E-4	0.0
15	3.2026800026261975E-4	0.0	0.011049246009060381	0.0011209380009191692	0.0
16	3.2026800026261975E-4	0.0	0.012010050009848242	0.0011209380009191692	0.0
17	3.2026800026261975E-4	0.0	0.012330318010110862	0.0014412060011817888	0.0
18	3.2026800026261975E-4	0.0	0.01265058601037348	0.0014412060011817888	0.0
19	3.2026800026261975E-4	0.0	0.012810720010504791	0.0014412060011817888	0.0
20	3.2026800026261975E-4	0.0	0.013130988010767411	0.0016013400013130989	0.0
21	3.2026800026261975E-4	0.0	0.01361139001116134	0.0022418760018383384	0.0
22	3.2026800026261975E-4	0.0	0.01377152401129265	0.002722278002232268	0.0
23	3.2026800026261975E-4	0.0	0.01377152401129265	0.0032026800026261977	0.0
24	3.2026800026261975E-4	0.0	0.01377152401129265	0.0036830820030201274	0.0
25	3.2026800026261975E-4	0.0	0.01377152401129265	0.004163484003414057	0.0
26	3.2026800026261975E-4	0.0	0.01377152401129265	0.004323618003545367	0.0
27	3.2026800026261975E-4	0.0	0.01409179201155527	0.0064053600052523954	0.0
28	3.2026800026261975E-4	0.0	0.014251926011686579	0.01697420401391885	0.0
29	3.2026800026261975E-4	0.0	0.014251926011686579	0.0389125620319083	0.0
30	3.2026800026261975E-4	0.0	0.014251926011686579	0.060850920049897755	0.0
31	3.2026800026261975E-4	0.0	0.014251926011686579	0.1199403660983511	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	540	0.0	28.092594	1
GGTAACG	65	9.377436E-8	25.615385	25
TGCGGGT	70	1.9199979E-7	23.785715	21
GTATCAA	1685	0.0	23.605341	1
TCGCCGT	40	0.0019302046	23.125002	25
CACGATA	40	0.0019302046	23.125002	16
CGCCGTC	40	0.0019302046	23.125002	26
GCGGGTA	65	2.6795879E-6	22.76923	22
GTATTGG	75	3.7351128E-7	22.2	1
CGTGGGA	105	9.804353E-10	21.142857	37
ACGTGGG	105	9.804353E-10	21.142857	36
TGCTAGC	105	9.804353E-10	21.142857	28
CGAGTCG	45	0.0038238796	20.555557	21
GGCGTCA	45	0.0038238796	20.555557	25
CCGTCCG	45	0.0038238796	20.555557	28
ACCCGTC	45	0.0038238796	20.555557	25
TGTCCGG	55	5.13948E-4	20.181818	14
TACCCTC	175	0.0	20.085714	5
CGGGTAA	75	9.25466E-6	19.733334	23
CAACCCA	210	0.0	19.38095	17
>>END_MODULE