FastQCFastQC Report
Fri 10 Feb 2017
ERR1633502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences728340
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50350.691298020155422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.4777988302166571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19140.26278935661916136No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG13260.18205783013427795No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11460.15734409753686465No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA11090.1522640525029519No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10340.14196666392069637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.12439245407364692No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG8050.11052530411620946No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG7910.10860312491418843No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7870.10805393085646814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24800.023.2741931
TCGTCAC400.001930511323.12500219
GGTATCA9400.022.2393631
TCTCGCG502.7007854E-422.220
CTATCGC450.003824482220.55555530
TTCGTCA450.003824482220.55555518
TACCGCA2250.019.7333347
TAGTACC2000.019.4250014
GCTCTCG500.007032468418.518
ACCGCAA1950.018.025648
GGACGAT1653.6379788E-1217.93939612
AACGTCA1552.0008883E-1117.90322528
AGCGGCT1258.561983E-917.769
ATAGTAC2500.017.763
CGCCGTT1258.561983E-917.7625
GACTGTT1156.395385E-817.6956527
GACGATG1800.017.47222113
AGGACCG1705.456968E-1217.4117645
GTCTATA852.7208991E-517.4117641
CGGTCTG1705.456968E-1217.41176410
Produced by FastQC (version 0.11.5)