##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633502.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728340
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.832123733421206	33.0	33.0	33.0	27.0	33.0
2	31.94733503583491	33.0	33.0	33.0	33.0	33.0
3	31.6729219869841	33.0	33.0	33.0	27.0	33.0
4	31.954559683664222	33.0	33.0	33.0	33.0	33.0
5	32.060117527528355	33.0	33.0	33.0	33.0	33.0
6	35.220449240739214	37.0	37.0	37.0	33.0	37.0
7	35.37756267677184	37.0	37.0	37.0	33.0	37.0
8	35.52374303210039	37.0	37.0	37.0	33.0	37.0
9	35.63889392316775	37.0	37.0	37.0	33.0	37.0
10	35.64961693714474	37.0	37.0	37.0	33.0	37.0
11	35.66852294258176	37.0	37.0	37.0	33.0	37.0
12	35.637752972512835	37.0	37.0	37.0	33.0	37.0
13	35.65154460828734	37.0	37.0	37.0	33.0	37.0
14	35.63152511189829	37.0	37.0	37.0	33.0	37.0
15	35.646546942362086	37.0	37.0	37.0	33.0	37.0
16	35.62166295960678	37.0	37.0	37.0	33.0	37.0
17	35.630310020045584	37.0	37.0	37.0	33.0	37.0
18	35.60857841118159	37.0	37.0	37.0	33.0	37.0
19	35.59367191146992	37.0	37.0	37.0	33.0	37.0
20	35.59793365735783	37.0	37.0	37.0	33.0	37.0
21	35.59062388444957	37.0	37.0	37.0	33.0	37.0
22	35.503702940934176	37.0	37.0	37.0	33.0	37.0
23	35.54300464068979	37.0	37.0	37.0	33.0	37.0
24	35.53738226652388	37.0	37.0	37.0	33.0	37.0
25	35.55808688249993	37.0	37.0	37.0	33.0	37.0
26	35.454315292308536	37.0	37.0	37.0	33.0	37.0
27	35.458325781915036	37.0	37.0	37.0	33.0	37.0
28	35.484078864266685	37.0	37.0	37.0	33.0	37.0
29	35.48749485130571	37.0	37.0	37.0	33.0	37.0
30	35.48686877007991	37.0	37.0	37.0	33.0	37.0
31	35.47660296015597	37.0	37.0	37.0	33.0	37.0
32	35.46667765054782	37.0	37.0	37.0	33.0	37.0
33	35.45700634319137	37.0	37.0	37.0	33.0	37.0
34	35.43015487272428	37.0	37.0	37.0	33.0	37.0
35	35.3600694730483	37.0	37.0	37.0	33.0	37.0
36	35.392872834115934	37.0	37.0	37.0	33.0	37.0
37	35.38560425076201	37.0	37.0	37.0	33.0	37.0
38	35.37044512178378	37.0	37.0	37.0	33.0	37.0
39	35.26512480434962	37.0	37.0	37.0	33.0	37.0
40	35.04575884888926	37.0	37.0	37.0	27.0	37.0
41	35.21280720542604	37.0	37.0	37.0	33.0	37.0
42	35.2371351291979	37.0	37.0	37.0	33.0	37.0
43	34.90770519262981	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	30.0
16	55.0
17	38.0
18	36.0
19	22.0
20	42.0
21	123.0
22	302.0
23	730.0
24	1514.0
25	2791.0
26	4619.0
27	7050.0
28	9987.0
29	13257.0
30	18096.0
31	23351.0
32	29998.0
33	39265.0
34	56473.0
35	113687.0
36	406872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66853392646291	20.542741027542082	13.441249965675372	23.34747508031963
2	16.331383694428425	22.220528873877583	38.8554795837109	22.592607847983086
3	18.20180135650932	27.487849081472937	29.919268473515114	24.391081088502624
4	12.083504956476371	18.054754647554713	40.03981656918472	29.821923826784197
5	14.110854820550841	36.8603948705275	34.89743800972073	14.131312299200923
6	29.04961968311503	40.35299448059972	17.312106983002444	13.285278853282808
7	26.934810665348603	32.335310431941124	22.943405552351923	17.78647335035835
8	24.69904165636928	35.172584232638606	20.844660460773813	19.283713650218303
9	26.322733888019336	14.951670922920615	20.36960760084576	38.3559875882143
10	15.134964439684762	27.93956119394788	33.585138808798085	23.340335557569265
11	33.973007112063044	24.23977812560068	23.747837548397726	18.039377213938547
12	23.12258011368317	25.642557047532744	30.507180712304695	20.72768212647939
13	28.431227174121975	22.704231540214735	26.146167998462253	22.718373287201032
14	22.316912431007495	21.29664717027762	28.29049619683115	28.09594420188374
15	23.82692149270945	28.802345058626464	25.438943350632947	21.93179009803114
16	22.957410000823792	28.543811955954634	25.14443803718044	23.354340006041134
17	22.648762940384984	27.155586676552158	26.24364994370761	23.952000439355245
18	22.927067029134747	26.36996457698328	28.6171293626603	22.08583903122168
19	25.33198780789192	26.160996238020708	27.83068347200483	20.676332482082543
20	24.71483098552874	25.965895049015568	28.002169316527997	21.3171046489277
21	23.02853063129857	26.88167614026416	27.911277699975283	22.178515528461983
22	23.055578438641295	26.270423154021472	28.066699618310132	22.6072987890271
23	22.34505862646566	26.498338687975398	28.863854793091136	22.292747892467805
24	22.740890243567566	27.223137545651753	27.29686684790071	22.739105362879975
25	22.903451684652772	27.165197572562267	28.328939780871572	21.602410961913392
26	23.654611857099706	26.610511574264766	27.50940494823846	22.225471620397066
27	23.242990910838344	26.63481341131889	27.409588928247796	22.71260674959497
28	22.56761951835681	26.5262102864047	28.355575692671003	22.55059450256748
29	23.816898701156052	26.871790647225197	27.510777933382762	21.80053271823599
30	23.41379026278936	26.43023862481808	27.838921382870634	22.317049729521926
31	22.968531180492626	26.018205783013425	28.058736304473186	22.95452673202076
32	21.87879287146113	26.52470000274597	28.263448389488428	23.333058736304473
33	22.299887415218166	26.292802811873578	28.680561276326987	22.726748496581266
34	22.737595079221244	26.48296125435923	28.344729110031032	22.4347145563885
35	22.892742400527226	27.193481066534858	27.32569953593102	22.588076997006894
36	23.154433369030947	26.500672762720708	27.40299859955515	22.941895268693195
37	23.16212208583903	26.310651618749485	27.847845786308596	22.67938050910289
38	22.932147074168657	26.328363127110965	27.35178625367273	23.387703545047643
39	22.655078672048766	26.36419803937721	28.06340445396381	22.91731883461021
40	23.01301589916797	27.249636158936763	27.683911360079083	22.053436581816186
41	22.847708487794165	26.18159101518522	27.899195430705443	23.071505066315183
42	21.923689485679766	27.872284921877146	27.663728478457863	22.54029711398523
43	23.428069308290084	25.94282889859132	27.33997858143175	23.28912321168685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	50.0
2	70.0
3	302.5
4	535.0
5	535.0
6	670.5
7	806.0
8	754.5
9	703.0
10	947.5
11	1192.0
12	1192.0
13	1859.0
14	2526.0
15	4520.0
16	6514.0
17	7442.0
18	8370.0
19	8370.0
20	7612.0
21	6854.0
22	7279.0
23	7704.0
24	9402.5
25	11101.0
26	11101.0
27	13444.0
28	15787.0
29	17982.5
30	20178.0
31	23332.5
32	26487.0
33	26487.0
34	32912.0
35	39337.0
36	41694.0
37	44051.0
38	45386.0
39	46721.0
40	46721.0
41	48079.5
42	49438.0
43	51476.0
44	53514.0
45	53036.5
46	52559.0
47	52559.0
48	51955.5
49	51352.0
50	52348.5
51	53345.0
52	53706.0
53	54067.0
54	54067.0
55	51536.5
56	49006.0
57	42192.5
58	35379.0
59	31884.5
60	28390.0
61	28390.0
62	25370.5
63	22351.0
64	17462.5
65	12574.0
66	10558.5
67	8543.0
68	8543.0
69	7371.0
70	6199.0
71	5876.5
72	5554.0
73	4889.0
74	4224.0
75	4224.0
76	2692.0
77	1160.0
78	930.0
79	700.0
80	582.5
81	465.0
82	465.0
83	351.0
84	237.0
85	196.0
86	155.0
87	126.0
88	97.0
89	97.0
90	71.5
91	46.0
92	30.5
93	15.0
94	9.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	728340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.967842061047286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.64770312907366	46.7618955742236
2	7.658002734815486	8.265717641911808
3	1.9633489176435266	3.17873112894342
4	0.8991021403723956	1.94090409233468
5	0.5371793154549325	1.4495204227466647
6	0.3328640910288048	1.0778374015461964
7	0.2470124611840721	0.9331510644604805
8	0.191542292971317	0.8269699372069506
9	0.14138752643011535	0.6867341726204352
>10	1.097343030906616	12.05254003157289
>50	0.15232624222325833	5.772615662528498
>100	0.1249590801984032	13.043625269490189
>500	0.0054217782730654794	1.9136149448915334
>1k	0.0015490795065901369	1.3945953096999677
>5k	2.581799177650228E-4	0.7015473458226933
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5035	0.691298020155422	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3480	0.4777988302166571	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1914	0.26278935661916136	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1326	0.18205783013427795	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1146	0.15734409753686465	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1109	0.1522640525029519	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1034	0.14196666392069637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.12439245407364692	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	805	0.11052530411620946	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	791	0.10860312491418843	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	787	0.10805393085646814	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.3729851443007387E-4	0.0	0.0
2	1.3729851443007387E-4	0.0	2.7459702886014774E-4	0.0	0.0
3	1.3729851443007387E-4	0.0	2.7459702886014774E-4	0.0	0.0
4	1.3729851443007387E-4	0.0	4.118955432902216E-4	0.0	0.0
5	1.3729851443007387E-4	0.0	4.118955432902216E-4	0.0	0.0
6	1.3729851443007387E-4	0.0	8.237910865804432E-4	0.0	0.0
7	1.3729851443007387E-4	0.0	0.0023340747453112556	0.0	0.0
8	1.3729851443007387E-4	0.0	0.003020567317461625	0.0	0.0
9	1.3729851443007387E-4	0.0	0.003432462860751847	2.7459702886014774E-4	0.0
10	2.7459702886014774E-4	0.0	0.0037070598896119943	2.7459702886014774E-4	0.0
11	2.7459702886014774E-4	0.0	0.006453030178213472	4.118955432902216E-4	0.0
12	2.7459702886014774E-4	0.0	0.007276821264793915	4.118955432902216E-4	0.0
13	2.7459702886014774E-4	0.0	0.007963313836944284	4.118955432902216E-4	0.0
14	2.7459702886014774E-4	0.0	0.009199000466814948	4.118955432902216E-4	0.0
15	2.7459702886014774E-4	0.0	0.009610896010105171	5.491940577202955E-4	0.0
16	2.7459702886014774E-4	0.0	0.010846582639975835	5.491940577202955E-4	0.0
17	4.118955432902216E-4	0.0	0.011395776697696132	6.864925721503693E-4	0.0
18	5.491940577202955E-4	0.0	0.011670373726556278	9.610896010105171E-4	0.0
19	5.491940577202955E-4	0.0	0.011807672240986352	0.001098388115440591	0.0
20	5.491940577202955E-4	0.0	0.012494164813136721	0.001098388115440591	0.0
21	5.491940577202955E-4	0.0	0.012906060356426944	0.0013729851443007386	0.0
22	5.491940577202955E-4	0.0	0.012906060356426944	0.0017848806875909603	0.0
23	5.491940577202955E-4	0.0	0.01318065738528709	0.002883268803031551	0.0
24	5.491940577202955E-4	0.0	0.01318065738528709	0.0035697613751819205	0.0
25	5.491940577202955E-4	0.0	0.01318065738528709	0.0037070598896119943	0.0
26	5.491940577202955E-4	0.0	0.01318065738528709	0.004118955432902216	0.0
27	5.491940577202955E-4	0.0	0.01318065738528709	0.00604113463492325	0.0
28	5.491940577202955E-4	0.0	0.01318065738528709	0.019221792020210342	0.0
29	5.491940577202955E-4	0.0	0.01318065738528709	0.04379822610319356	0.0
30	6.864925721503693E-4	0.0	0.01318065738528709	0.07331740670565945	0.0
31	6.864925721503693E-4	0.0	0.01318065738528709	0.13798500700222424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2480	0.0	23.274193	1
TCGTCAC	40	0.0019305113	23.125002	19
GGTATCA	940	0.0	22.239363	1
TCTCGCG	50	2.7007854E-4	22.2	20
CTATCGC	45	0.0038244822	20.555555	30
TTCGTCA	45	0.0038244822	20.555555	18
TACCGCA	225	0.0	19.733334	7
TAGTACC	200	0.0	19.425001	4
GCTCTCG	50	0.0070324684	18.5	18
ACCGCAA	195	0.0	18.02564	8
GGACGAT	165	3.6379788E-12	17.939396	12
AACGTCA	155	2.0008883E-11	17.903225	28
AGCGGCT	125	8.561983E-9	17.76	9
ATAGTAC	250	0.0	17.76	3
CGCCGTT	125	8.561983E-9	17.76	25
GACTGTT	115	6.395385E-8	17.695652	7
GACGATG	180	0.0	17.472221	13
AGGACCG	170	5.456968E-12	17.411764	5
GTCTATA	85	2.7208991E-5	17.411764	1
CGGTCTG	170	5.456968E-12	17.411764	10
>>END_MODULE