FastQCFastQC Report
Fri 10 Feb 2017
ERR1633501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572347
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23450.4097164831824051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.3120484601124842No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16130.28182204152376095No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10460.18275626499308986No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10210.17838828542824545No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9310.1626635589948056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8810.15392759986511678No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8360.14606523664839685No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7850.13715455833611429No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7090.12387590045898729No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7010.12247814699823709No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6260.10937420830370388No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6120.106928139747391No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6110.10675342056479724No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5840.10203600263476527No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5810.10151184508698395No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5800.10133712590439017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGC250.00549406729.59999817
GTCGGTA451.3220782E-424.66666820
GGTATCA5700.024.3421041
TGCACCG753.733985E-722.25
GTATCAA14100.021.255321
CGTCGGT555.1387574E-420.18181819
CGATAAG555.1387574E-420.18181812
AACGGCC1850.020.037
CTATCCC609.229093E-418.59
GCGGGGT1103.8415237E-818.529
GCGATAA609.229093E-418.511
AGGTCGC1700.018.535
ATCAAGC4000.018.49999830
TAAGGTC500.00703067318.4999985
TCAAGCA4050.018.27160531
GTTTAAC2700.017.81481633
TTTAACG2350.017.31914934
AGCGTCG752.0649815E-417.26666617
GTTTAGG1502.5102054E-1017.2666661
GCATCAG4500.017.26666615
Produced by FastQC (version 0.11.5)