##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633501.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572347
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69135157518079	33.0	33.0	33.0	27.0	33.0
2	31.84201716790688	33.0	33.0	33.0	33.0	33.0
3	31.553428252441265	33.0	33.0	33.0	27.0	33.0
4	31.853300532718787	33.0	33.0	33.0	27.0	33.0
5	31.950905656882973	33.0	33.0	33.0	33.0	33.0
6	35.06900184678176	37.0	37.0	37.0	33.0	37.0
7	35.225148380265814	37.0	37.0	37.0	33.0	37.0
8	35.35827391425132	37.0	37.0	37.0	33.0	37.0
9	35.49635623144701	37.0	37.0	37.0	33.0	37.0
10	35.495984079588084	37.0	37.0	37.0	33.0	37.0
11	35.50966983316065	37.0	37.0	37.0	33.0	37.0
12	35.515044195217236	37.0	37.0	37.0	33.0	37.0
13	35.49740454654257	37.0	37.0	37.0	33.0	37.0
14	35.49405343262042	37.0	37.0	37.0	33.0	37.0
15	35.485247585817696	37.0	37.0	37.0	33.0	37.0
16	35.47635437942367	37.0	37.0	37.0	33.0	37.0
17	35.465897436345436	37.0	37.0	37.0	33.0	37.0
18	35.45364962164561	37.0	37.0	37.0	33.0	37.0
19	35.43551901206786	37.0	37.0	37.0	33.0	37.0
20	35.43309216262163	37.0	37.0	37.0	33.0	37.0
21	35.444457645449354	37.0	37.0	37.0	33.0	37.0
22	35.34357828380336	37.0	37.0	37.0	33.0	37.0
23	35.39253634595796	37.0	37.0	37.0	33.0	37.0
24	35.37420655651204	37.0	37.0	37.0	33.0	37.0
25	35.40519824512053	37.0	37.0	37.0	33.0	37.0
26	35.28811717367262	37.0	37.0	37.0	33.0	37.0
27	35.29225801830009	37.0	37.0	37.0	33.0	37.0
28	35.3273066863284	37.0	37.0	37.0	33.0	37.0
29	35.32820649011876	37.0	37.0	37.0	33.0	37.0
30	35.34038092276189	37.0	37.0	37.0	33.0	37.0
31	35.31050568973018	37.0	37.0	37.0	33.0	37.0
32	35.31138103283497	37.0	37.0	37.0	33.0	37.0
33	35.31263901094965	37.0	37.0	37.0	33.0	37.0
34	35.286406672875025	37.0	37.0	37.0	33.0	37.0
35	35.2218933618941	37.0	37.0	37.0	33.0	37.0
36	35.261965206421976	37.0	37.0	37.0	33.0	37.0
37	35.23561231211136	37.0	37.0	37.0	33.0	37.0
38	35.22591015590193	37.0	37.0	37.0	33.0	37.0
39	35.136957125659784	37.0	37.0	37.0	33.0	37.0
40	34.92284750335024	37.0	37.0	37.0	27.0	37.0
41	35.10547098176456	37.0	37.0	37.0	27.0	37.0
42	35.143077538626045	37.0	37.0	37.0	33.0	37.0
43	34.79533394950965	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	22.0
16	23.0
17	23.0
18	16.0
19	21.0
20	50.0
21	132.0
22	317.0
23	738.0
24	1508.0
25	2746.0
26	4347.0
27	6229.0
28	8881.0
29	11953.0
30	15539.0
31	19618.0
32	24796.0
33	32797.0
34	47042.0
35	94225.0
36	301323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.969551688049386	20.47132246696497	13.48832089623952	25.07080494874613
2	16.408752033294487	21.015223282379395	38.741183233248364	23.834841451077754
3	17.68175599767274	27.24658292958642	29.559515468762832	25.512145603978002
4	12.577160359012977	17.512103671374184	39.398302078983555	30.512433890629286
5	13.804213178369066	37.43638037763804	35.08151523463913	13.677891209353765
6	29.90738136130704	39.611459481747964	17.069365262681554	13.411793894263447
7	27.11047668634587	31.914031173396555	23.206376551287942	17.769115588969626
8	24.80330988019506	35.474633395475124	20.24418752959306	19.477869194736762
9	26.118595886761003	15.181175056390616	19.89003174647548	38.8101973103729
10	15.522052181631075	27.69316516029611	33.32558744957168	23.45919520850114
11	33.97501865127274	23.672527330448137	23.703277906584642	18.64917611169448
12	23.715508249366206	25.573297317885828	29.338146264416515	21.373048168331447
13	27.277158786540333	22.065635008133178	26.016909322491426	24.640296882835063
14	21.927082696336313	20.703698979814693	27.676916276314895	29.692302047534103
15	24.32615179253145	28.167702460220813	24.687995219683163	22.81815052756457
16	23.02431916302523	28.035090600632135	24.712980062794074	24.227610173548562
17	22.51169308129509	27.471271798402018	25.88464690126794	24.132388219034954
18	22.748262854527063	27.114495227545525	27.778777559767065	22.358464358160347
19	25.190662308005457	25.80742102256149	26.80000069887673	22.201915970556325
20	23.98212972200431	26.270951013982774	27.3931723237826	22.353746940230316
21	23.708519482062453	26.66162310626246	27.10820533697215	22.52165207470293
22	23.072891095786296	26.835818131308454	26.804718116806765	23.286572656098485
23	23.04703265676242	26.75142876611566	27.310530150415747	22.89100842670618
24	23.46950364027417	27.193992455625693	25.88586993554609	23.45063396855404
25	23.3199440199739	26.75771865668904	27.298823965181963	22.623513358155105
26	23.481384544690545	26.840360830055893	27.427592002753578	22.250662622499988
27	23.63059472662563	26.708622566380186	26.132748140551097	23.528034566443086
28	22.462596990986235	26.464889306661867	27.884832103601486	23.18768159875041
29	24.39726249984712	26.107938016622782	26.70932144311056	22.785478040419534
30	23.572762677187093	26.005552575622833	27.540111156343965	22.881573590846113
31	23.014185450434788	26.426800524856425	27.429339194579512	23.129674830129275
32	22.03575802790964	26.248761677793365	27.557408355420748	24.15807193887624
33	22.497016669957212	26.567100028479228	27.82665061579776	23.109232685765804
34	23.31452772531349	26.569371377852946	27.512854963859333	22.60324593297423
35	23.44259688615473	26.838089480682175	26.883516468156554	22.835797165006543
36	24.243859057529786	26.638210735794893	26.245092574958896	22.872837631716425
37	23.045984341666855	26.240200437846273	26.872858598018333	23.840956622468536
38	23.744686352859368	26.51206348596219	26.664243894001366	23.07900626717708
39	22.64447966006636	26.69499447013787	27.141926139212753	23.51859973058302
40	23.026590512398947	27.0225929375012	27.50376956636446	22.44704698373539
41	22.563409959342845	25.9755008762167	27.62816962437123	23.832919540069224
42	22.25171093759555	27.222471682388484	27.151710413438003	23.37410696657797
43	23.441548571059165	25.392113525536082	27.355258261159747	23.811079642245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	39.0
2	35.0
3	154.0
4	273.0
5	273.0
6	331.0
7	389.0
8	375.0
9	361.0
10	443.0
11	525.0
12	525.0
13	912.5
14	1300.0
15	2280.5
16	3261.0
17	3667.0
18	4073.0
19	4073.0
20	3866.5
21	3660.0
22	3961.5
23	4263.0
24	5554.5
25	6846.0
26	6846.0
27	8772.5
28	10699.0
29	12773.5
30	14848.0
31	17827.5
32	20807.0
33	20807.0
34	25544.5
35	30282.0
36	32996.0
37	35710.0
38	38405.0
39	41100.0
40	41100.0
41	42770.0
42	44440.0
43	45195.5
44	45951.0
45	44418.0
46	42885.0
47	42885.0
48	40971.5
49	39058.0
50	39762.0
51	40466.0
52	39020.0
53	37574.0
54	37574.0
55	37765.5
56	37957.0
57	32929.5
58	27902.0
59	24852.0
60	21802.0
61	21802.0
62	20051.0
63	18300.0
64	14685.0
65	11070.0
66	8983.0
67	6896.0
68	6896.0
69	5968.5
70	5041.0
71	4477.5
72	3914.0
73	4306.5
74	4699.0
75	4699.0
76	4100.5
77	3502.0
78	2536.5
79	1571.0
80	950.0
81	329.0
82	329.0
83	261.0
84	193.0
85	183.0
86	173.0
87	133.5
88	94.0
89	94.0
90	68.5
91	43.0
92	27.5
93	12.0
94	6.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	572347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.82753972709428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7270089360427	45.81574509980857
2	7.337908312072387	7.752872857395589
3	1.954998541985017	3.098332894293747
4	0.9181936967625709	1.9402365597156912
5	0.5478651313762142	1.4471183496433344
6	0.3616098185596152	1.1461774233399256
7	0.262181323635961	0.9695276003056629
8	0.19838312161719823	0.8384073790734013
9	0.17829260077153775	0.8476883505274834
>10	1.2065404954144063	12.930462314993637
>50	0.16679182802899786	6.242452324593454
>100	0.13114679989455316	13.13206608455442
>500	0.0073980246094363334	2.4513261082581392
>1k	0.0016813692294173484	1.3875866534969437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2345	0.4097164831824051	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1786	0.3120484601124842	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1613	0.28182204152376095	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1046	0.18275626499308986	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1021	0.17838828542824545	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	931	0.1626635589948056	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	881	0.15392759986511678	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	836	0.14606523664839685	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	785	0.13715455833611429	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	709	0.12387590045898729	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	701	0.12247814699823709	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	626	0.10937420830370388	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	612	0.106928139747391	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	611	0.10675342056479724	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	584	0.10203600263476527	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	581	0.10151184508698395	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	580	0.10133712590439017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	1.7471918259377616E-4	0.0	0.0
6	0.0	0.0	6.988767303751047E-4	0.0	0.0
7	0.0	0.0	0.002096630191125314	1.7471918259377616E-4	0.0
8	0.0	0.0	0.00227134937371909	1.7471918259377616E-4	0.0
9	0.0	0.0	0.003144945286687971	5.241575477813285E-4	0.0
10	0.0	0.0	0.003319664469281747	6.988767303751047E-4	0.0
11	0.0	0.0	0.00454269874743818	6.988767303751047E-4	0.0
12	0.0	0.0	0.00454269874743818	6.988767303751047E-4	0.0
13	0.0	0.0	0.00454269874743818	6.988767303751047E-4	0.0
14	0.0	0.0	0.005241575477813285	8.735959129688807E-4	0.0
15	0.0	0.0	0.005416294660407061	0.001048315095562657	0.0
16	0.0	0.0	0.006115171390782165	0.001048315095562657	0.0
17	0.0	0.0	0.006464609755969718	0.0013977534607502093	0.0
18	0.0	0.0	0.006464609755969718	0.0015724726433439854	0.0
19	0.0	0.0	0.006464609755969718	0.0015724726433439854	0.0
20	0.0	0.0	0.006639328938563494	0.0017471918259377615	0.0
21	0.0	0.0	0.007163486486344822	0.0019219110085315378	1.7471918259377616E-4
22	0.0	0.0	0.007163486486344822	0.0026207877389066423	1.7471918259377616E-4
23	0.0	0.0	0.007163486486344822	0.003319664469281747	1.7471918259377616E-4
24	0.0	0.0	0.007163486486344822	0.004367979564844404	1.7471918259377616E-4
25	0.0	0.0	0.007163486486344822	0.00454269874743818	1.7471918259377616E-4
26	0.0	0.0	0.007163486486344822	0.004892137112625732	1.7471918259377616E-4
27	0.0	0.0	0.007163486486344822	0.006464609755969718	1.7471918259377616E-4
28	0.0	0.0	0.007163486486344822	0.016248883981221183	1.7471918259377616E-4
29	0.0	0.0	0.007163486486344822	0.03040113777131705	1.7471918259377616E-4
30	0.0	0.0	0.007163486486344822	0.053289350691101724	1.7471918259377616E-4
31	0.0	0.0	0.007163486486344822	0.10815117402554744	1.7471918259377616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCGC	25	0.005494067	29.599998	17
GTCGGTA	45	1.3220782E-4	24.666668	20
GGTATCA	570	0.0	24.342104	1
TGCACCG	75	3.733985E-7	22.2	5
GTATCAA	1410	0.0	21.25532	1
CGTCGGT	55	5.1387574E-4	20.181818	19
CGATAAG	55	5.1387574E-4	20.181818	12
AACGGCC	185	0.0	20.0	37
CTATCCC	60	9.229093E-4	18.5	9
GCGGGGT	110	3.8415237E-8	18.5	29
GCGATAA	60	9.229093E-4	18.5	11
AGGTCGC	170	0.0	18.5	35
ATCAAGC	400	0.0	18.499998	30
TAAGGTC	50	0.007030673	18.499998	5
TCAAGCA	405	0.0	18.271605	31
GTTTAAC	270	0.0	17.814816	33
TTTAACG	235	0.0	17.319149	34
AGCGTCG	75	2.0649815E-4	17.266666	17
GTTTAGG	150	2.5102054E-10	17.266666	1
GCATCAG	450	0.0	17.266666	15
>>END_MODULE