FastQCFastQC Report
Fri 10 Feb 2017
ERR1633500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593846
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29050.48918406455545715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.35935242470270073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12010.20224098503652463No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9250.15576428905810596No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7990.1345466669810018No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5950.10019432647521412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAG451.3221602E-424.66666628
GCTCGGT551.9006517E-523.54545426
GGTATCA6150.022.5609761
TGCTCGC753.734458E-722.210
ATCTCGT502.6999717E-422.19999937
CTCGGTA502.6999717E-422.19999927
TAGGGGT1005.329639E-1022.1999994
GTATCAA15100.021.8079471
CCGGCAG806.948467E-720.812516
GCCGGCA909.458927E-820.55555515
ATAGTAC2200.020.1818183
CTGCTCG1850.020.09
TAGTACC1650.019.0606064
GCCCACG801.614527E-518.518
TTTGTCG609.2296506E-418.536
AGCTTCG801.614527E-518.521
GTGCTAT609.2296506E-418.51
CGGTAGG609.2296506E-418.529
CGCAAGG1501.2732926E-1118.510
TAGTACT801.614527E-518.54
Produced by FastQC (version 0.11.5)