##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633500.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593846
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11912684433338	33.0	33.0	33.0	27.0	33.0
2	31.41544609208448	33.0	33.0	33.0	27.0	33.0
3	31.1594773729216	33.0	33.0	33.0	27.0	33.0
4	31.5065269446961	33.0	33.0	33.0	27.0	33.0
5	31.61073577998336	33.0	33.0	33.0	27.0	33.0
6	34.42827264981157	37.0	37.0	37.0	27.0	37.0
7	34.60111544070348	37.0	37.0	37.0	27.0	37.0
8	34.73173853153848	37.0	37.0	37.0	27.0	37.0
9	34.882151264806026	37.0	37.0	37.0	27.0	37.0
10	34.86025838348663	37.0	37.0	37.0	27.0	37.0
11	34.88096914014744	37.0	37.0	37.0	27.0	37.0
12	34.89140450554521	37.0	37.0	37.0	27.0	37.0
13	34.89620878140124	37.0	37.0	37.0	27.0	37.0
14	34.89542743404856	37.0	37.0	37.0	27.0	37.0
15	34.90854868097116	37.0	37.0	37.0	27.0	37.0
16	34.890874064993284	37.0	37.0	37.0	27.0	37.0
17	34.88524634332807	37.0	37.0	37.0	27.0	37.0
18	34.858704108472566	37.0	37.0	37.0	27.0	37.0
19	34.83749995790154	37.0	37.0	37.0	27.0	37.0
20	34.83902560596518	37.0	37.0	37.0	27.0	37.0
21	34.84353856050222	37.0	37.0	37.0	27.0	37.0
22	34.72165174136055	37.0	37.0	37.0	27.0	37.0
23	34.77651781775073	37.0	37.0	37.0	27.0	37.0
24	34.751822863166545	37.0	37.0	37.0	27.0	37.0
25	34.7728906147385	37.0	37.0	37.0	27.0	37.0
26	34.65539382264089	37.0	37.0	37.0	27.0	37.0
27	34.65205625700939	37.0	37.0	37.0	27.0	37.0
28	34.65900755414703	37.0	37.0	37.0	27.0	37.0
29	34.65721752777656	37.0	37.0	37.0	27.0	37.0
30	34.670948360349314	37.0	37.0	37.0	27.0	37.0
31	34.655210273370535	37.0	37.0	37.0	27.0	37.0
32	34.65523216456792	37.0	37.0	37.0	27.0	37.0
33	34.63775456936647	37.0	37.0	37.0	27.0	37.0
34	34.61233046951567	37.0	37.0	37.0	27.0	37.0
35	34.536186149271025	37.0	37.0	37.0	27.0	37.0
36	34.570649629701975	37.0	37.0	37.0	27.0	37.0
37	34.55855726905629	37.0	37.0	37.0	27.0	37.0
38	34.540097937849204	37.0	37.0	37.0	27.0	37.0
39	34.44691889816552	37.0	37.0	37.0	27.0	37.0
40	34.22903917850756	37.0	37.0	37.0	27.0	37.0
41	34.41974181858596	37.0	37.0	37.0	27.0	37.0
42	34.428383789736735	37.0	37.0	37.0	27.0	37.0
43	34.01811412386376	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	17.0
16	34.0
17	40.0
18	39.0
19	63.0
20	146.0
21	339.0
22	859.0
23	1671.0
24	2993.0
25	4912.0
26	7288.0
27	10374.0
28	13789.0
29	17548.0
30	21656.0
31	27131.0
32	33272.0
33	43167.0
34	60652.0
35	111610.0
36	236245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.317614330988164	21.549526308167437	13.523202985285748	23.609656375558647
2	16.382698544740556	22.983399736632055	38.10701764430509	22.526884074322297
3	17.937647134105475	27.930641950943514	30.207663266234007	23.924047648717007
4	12.430158660662865	19.123644850685196	39.846189079323594	28.60000740932834
5	13.87952432112029	37.42906410079381	35.56612320365886	13.125288374427042
6	28.672248360686105	41.14298993341708	17.433812806687254	12.750948899209561
7	26.516975781599943	32.35838921201793	23.89205282177534	17.232582184606784
8	24.251573640304052	36.14405081452093	20.919901792720673	18.684473752454338
9	25.80029165810665	15.293695671941885	20.755549418536116	38.15046325141535
10	14.723345783250203	28.454009962178745	34.59971103619457	22.222933218376483
11	33.48157603149638	24.41895710335676	24.378710978940667	17.720755886206188
12	22.439319284797744	26.31793427925759	31.013764511337953	20.228981924606714
13	27.67889318106041	22.909643240840218	26.649501722668838	22.76196185543053
14	21.798075595356373	21.687272457842603	28.356341543093667	28.15831040370736
15	23.321366145431643	29.48727447856852	25.37930709308474	21.812052282915097
16	22.817700211839433	29.022002337306308	25.586936680553546	22.573360770300717
17	22.13317930911381	28.011470987427717	26.75424268244629	23.101107021012183
18	22.268399551398847	27.526159980870464	28.678142144596407	21.527298323134282
19	24.47132758324549	26.961367088437072	28.169087608571918	20.398217719745524
20	23.85955281335565	26.93139298740751	28.247053950013978	20.96200024922286
21	22.492531733816513	27.61018850004883	28.15948916048942	21.737790605645234
22	22.54136594335905	27.042196124921276	28.32451510997801	22.091922821741665
23	22.0137880864736	27.146263509394693	28.930732883609554	21.909215520522153
24	22.29786847095038	28.098867383126265	27.317856818097617	22.28540732782573
25	22.36051097422564	27.783970928489875	28.46125089669712	21.394267200587358
26	23.001586269841003	27.397001916321738	27.926263711467282	21.675148102369977
27	22.559552476567998	27.456444936902834	27.521613347568223	22.46238923896094
28	22.169383981705696	27.1829733634646	28.693634376589216	21.954008278240487
29	23.409941297912255	27.551419054771774	27.753154858330277	21.2854847889857
30	22.950394546734337	27.173206521556093	27.88837510061531	21.988023831094257
31	22.65031674878672	26.792973262428305	28.2802275337377	22.276482455047265
32	21.561819057466078	27.337390501914637	28.315590237199544	22.78520020341974
33	21.79959113979045	27.06021426430421	28.814372749837503	22.325821846067836
34	22.64374938957238	27.074190951862942	28.513789770411858	21.768269888152822
35	22.499099093030853	27.907235209128295	27.56842683119866	22.025238866642194
36	22.986094037848197	27.362817969641963	27.29343971332635	22.357648279183493
37	22.702687228675448	26.97551216982181	27.948491696500437	22.373308905002308
38	22.51610686945774	27.090861940637804	27.74574552998589	22.647285659918566
39	22.28439696486968	27.24881534943403	28.04531814645548	22.421469539240814
40	22.425005809587	27.742040865813696	28.18676896030284	21.646184364296467
41	22.471482505565415	26.942675373750095	27.981833674050176	22.604008446634314
42	21.726003037824622	28.44811617826844	27.753154858330277	22.072725925576663
43	22.948205426996225	26.598646787214193	27.706846556177865	22.746301229611717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	29.0
2	42.0
3	151.5
4	261.0
5	261.0
6	368.0
7	475.0
8	477.5
9	480.0
10	618.5
11	757.0
12	757.0
13	1253.0
14	1749.0
15	3058.0
16	4367.0
17	4972.5
18	5578.0
19	5578.0
20	5426.5
21	5275.0
22	5961.0
23	6647.0
24	8364.5
25	10082.0
26	10082.0
27	12536.0
28	14990.0
29	17262.5
30	19535.0
31	22365.0
32	25195.0
33	25195.0
34	30681.0
35	36167.0
36	38311.0
37	40455.0
38	41590.0
39	42725.0
40	42725.0
41	43774.5
42	44824.0
43	45532.5
44	46241.0
45	44975.5
46	43710.0
47	43710.0
48	41888.0
49	40066.0
50	40326.5
51	40587.0
52	39821.0
53	39055.0
54	39055.0
55	37533.5
56	36012.0
57	30548.0
58	25084.0
59	22636.5
60	20189.0
61	20189.0
62	18003.5
63	15818.0
64	12381.5
65	8945.0
66	7469.0
67	5993.0
68	5993.0
69	5107.0
70	4221.0
71	3964.0
72	3707.0
73	3234.5
74	2762.0
75	2762.0
76	1773.5
77	785.0
78	599.5
79	414.0
80	339.5
81	265.0
82	265.0
83	206.5
84	148.0
85	131.5
86	115.0
87	92.0
88	69.0
89	69.0
90	50.0
91	31.0
92	19.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	593846.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.01322159142598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.93525032638692	53.37290885126398
2	6.347894715177073	7.619152243619272
3	1.5413302326376457	2.775005783905416
4	0.6927021602511	1.662851529600349
5	0.43250596228173943	1.2978038077013478
6	0.29968312727279733	1.079096995454034
7	0.23016549834601147	0.9669081138458123
8	0.16037466378173304	0.7699680188146867
9	0.13563851321516385	0.732609373492187
>10	0.9952733205719306	11.993783586721644
>50	0.1338078242877717	5.711208120637299
>100	0.09195728603044176	10.00550580081164
>500	0.0025622773197336167	0.9470555259284889
>1k	8.540924399112055E-4	1.0661422482038194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2905	0.48918406455545715	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2134	0.35935242470270073	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.20224098503652463	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	925	0.15576428905810596	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	799	0.1345466669810018	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	595	0.10019432647521412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.683938260087632E-4	0.0	0.0
4	0.0	0.0	5.051814780262896E-4	0.0	0.0
5	0.0	0.0	6.735753040350528E-4	0.0	0.0
6	0.0	0.0	0.001683938260087632	0.0	0.0
7	0.0	0.0	0.003873057998201554	3.367876520175264E-4	0.0
8	0.0	0.0	0.004546633302236607	3.367876520175264E-4	0.0
9	0.0	0.0	0.005725390084297949	0.0010103629560525792	0.0
10	0.0	0.0	0.007072540692368055	0.0011787567820613425	0.0
11	1.683938260087632E-4	0.0	0.010608811038552083	0.0011787567820613425	0.0
12	1.683938260087632E-4	0.0	0.011282386342587136	0.0013471506080701056	0.0
13	1.683938260087632E-4	0.0	0.012124355472630952	0.0013471506080701056	0.0
14	1.683938260087632E-4	0.0	0.012966324602674767	0.001515544434078869	0.0
15	1.683938260087632E-4	0.0	0.014481869036753636	0.001515544434078869	0.0
16	1.683938260087632E-4	0.0	0.014818656688771163	0.001515544434078869	0.0
17	1.683938260087632E-4	0.0	0.015492231992806216	0.001515544434078869	0.0
18	1.683938260087632E-4	0.0	0.01566062581881498	0.0018523320860963954	0.0
19	1.683938260087632E-4	0.0	0.015997413470832507	0.0018523320860963954	0.0
20	1.683938260087632E-4	0.0	0.016670988774867557	0.0020207259121051585	0.0
21	1.683938260087632E-4	0.0	0.016670988774867557	0.002357513564122685	0.0
22	1.683938260087632E-4	0.0	0.017007776426885085	0.003031088868157738	0.0
23	1.683938260087632E-4	0.0	0.01717617025289385	0.0035362703461840274	0.0
24	1.683938260087632E-4	0.0	0.017512957904911374	0.00420984565021908	0.0
25	1.683938260087632E-4	0.0	0.017512957904911374	0.005220208606271659	0.0
26	1.683938260087632E-4	0.0	0.017512957904911374	0.006062177736315476	0.0
27	1.683938260087632E-4	0.0	0.017512957904911374	0.007409328344385581	0.0
28	1.683938260087632E-4	0.0	0.017512957904911374	0.016165807296841268	0.0
29	1.683938260087632E-4	0.0	0.017512957904911374	0.030479282507586143	0.0
30	1.683938260087632E-4	0.0	0.017512957904911374	0.05439120580083052	0.0
31	1.683938260087632E-4	0.0	0.017512957904911374	0.11484458933797652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTAG	45	1.3221602E-4	24.666666	28
GCTCGGT	55	1.9006517E-5	23.545454	26
GGTATCA	615	0.0	22.560976	1
TGCTCGC	75	3.734458E-7	22.2	10
ATCTCGT	50	2.6999717E-4	22.199999	37
CTCGGTA	50	2.6999717E-4	22.199999	27
TAGGGGT	100	5.329639E-10	22.199999	4
GTATCAA	1510	0.0	21.807947	1
CCGGCAG	80	6.948467E-7	20.8125	16
GCCGGCA	90	9.458927E-8	20.555555	15
ATAGTAC	220	0.0	20.181818	3
CTGCTCG	185	0.0	20.0	9
TAGTACC	165	0.0	19.060606	4
GCCCACG	80	1.614527E-5	18.5	18
TTTGTCG	60	9.2296506E-4	18.5	36
AGCTTCG	80	1.614527E-5	18.5	21
GTGCTAT	60	9.2296506E-4	18.5	1
CGGTAGG	60	9.2296506E-4	18.5	29
CGCAAGG	150	1.2732926E-11	18.5	10
TAGTACT	80	1.614527E-5	18.5	4
>>END_MODULE