FastQCFastQC Report
Fri 10 Feb 2017
ERR1633499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750942
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51620.6874032881367669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39080.5204130279036198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20140.26819647855626666No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11380.15154299533119733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.12957059266894114No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9530.1269072711341222No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7640.10173888263008328No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7530.10027405578593286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAT358.8665204E-426.428571
GGTATCA10050.023.378111
GTATCAA25450.022.6070731
ATCTCGC1103.846253E-818.511
CGAACTA1103.846253E-818.524
CGAGTCG609.232749E-418.521
GCGAACT1103.846253E-818.523
ACGGCGT609.232749E-418.535
AGCATCG500.007032667318.4999987
GTATAAC500.007032667318.4999981
TTTACGG650.001579244317.07692332
CGGTACA650.001579244317.07692333
GTTTATA2450.016.6122461
GTATTAG1504.660251E-916.0333331
TTAGGGC700.002591371515.8571423
AAAGCGA1403.4669938E-815.85714220
CGGCAAG2003.6379788E-1215.72499918
TCTCGCC1302.585275E-715.65384612
TGCTCGG1201.9332274E-615.41666710
TTGGCCG1455.3361873E-815.31034531
Produced by FastQC (version 0.11.5)