##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633499.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750942
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462045004807294	33.0	33.0	33.0	27.0	33.0
2	31.68594245627492	33.0	33.0	33.0	27.0	33.0
3	31.410789381869705	33.0	33.0	33.0	27.0	33.0
4	31.71353047239334	33.0	33.0	33.0	27.0	33.0
5	31.827059879458066	33.0	33.0	33.0	33.0	33.0
6	34.84084789504382	37.0	37.0	37.0	27.0	37.0
7	35.01374007579813	37.0	37.0	37.0	33.0	37.0
8	35.14626695537072	37.0	37.0	37.0	33.0	37.0
9	35.28046506920641	37.0	37.0	37.0	33.0	37.0
10	35.285304324435174	37.0	37.0	37.0	33.0	37.0
11	35.28755216781056	37.0	37.0	37.0	33.0	37.0
12	35.297345201094096	37.0	37.0	37.0	33.0	37.0
13	35.296967009436145	37.0	37.0	37.0	33.0	37.0
14	35.28186997131603	37.0	37.0	37.0	33.0	37.0
15	35.29272300657041	37.0	37.0	37.0	33.0	37.0
16	35.259071938977975	37.0	37.0	37.0	33.0	37.0
17	35.27041768871631	37.0	37.0	37.0	33.0	37.0
18	35.25607703391207	37.0	37.0	37.0	33.0	37.0
19	35.240430019895015	37.0	37.0	37.0	33.0	37.0
20	35.2376681554634	37.0	37.0	37.0	33.0	37.0
21	35.22987128167022	37.0	37.0	37.0	33.0	37.0
22	35.12287367066964	37.0	37.0	37.0	33.0	37.0
23	35.175225250418805	37.0	37.0	37.0	33.0	37.0
24	35.16269032761518	37.0	37.0	37.0	33.0	37.0
25	35.184978333879315	37.0	37.0	37.0	33.0	37.0
26	35.07028771862541	37.0	37.0	37.0	33.0	37.0
27	35.08856209933656	37.0	37.0	37.0	33.0	37.0
28	35.09684636097062	37.0	37.0	37.0	33.0	37.0
29	35.09652809404721	37.0	37.0	37.0	33.0	37.0
30	35.10313845809663	37.0	37.0	37.0	33.0	37.0
31	35.08647538691404	37.0	37.0	37.0	33.0	37.0
32	35.075868708901616	37.0	37.0	37.0	33.0	37.0
33	35.06352021860543	37.0	37.0	37.0	33.0	37.0
34	35.02419627614383	37.0	37.0	37.0	27.0	37.0
35	34.95622298393218	37.0	37.0	37.0	27.0	37.0
36	34.96894034426094	37.0	37.0	37.0	27.0	37.0
37	34.97359849362534	37.0	37.0	37.0	27.0	37.0
38	34.95401908536212	37.0	37.0	37.0	27.0	37.0
39	34.83867462467141	37.0	37.0	37.0	27.0	37.0
40	34.61766687706907	37.0	37.0	37.0	27.0	37.0
41	34.787361740320826	37.0	37.0	37.0	27.0	37.0
42	34.81846933584751	37.0	37.0	37.0	27.0	37.0
43	34.44309014544399	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	25.0
16	29.0
17	36.0
18	27.0
19	34.0
20	90.0
21	220.0
22	643.0
23	1409.0
24	2664.0
25	4533.0
26	7134.0
27	10141.0
28	13833.0
29	17799.0
30	22957.0
31	28882.0
32	36211.0
33	47029.0
34	66606.0
35	130941.0
36	359695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34380551360824	21.032782824772088	14.200697257577815	23.422714404041855
2	15.656468808509846	23.0234825059725	39.41369639732496	21.906352288192696
3	17.999792260920284	27.965009281675552	31.298156182501447	22.737042274902723
4	12.481789539005675	18.36759696487878	40.00921509251047	29.141398403605073
5	13.513427135517789	37.75804256520477	35.06422599881216	13.664304300465282
6	27.845026646531956	40.94803593353415	17.677397189130453	13.529540230803445
7	24.889671905420123	33.46689890830451	23.77773516463322	17.86569402164215
8	24.07562767830272	35.6695989836765	21.2289364558115	19.02583688220928
9	25.82143494437653	15.383345185114164	20.808930649770556	37.98628922073875
10	14.459438944685473	28.583698874213987	34.175209270489596	22.781652910610937
11	32.63234710536899	24.593776882901743	24.71961882542194	18.054257186307332
12	22.277486144069716	26.664376210146724	31.12317595766384	19.93496168811972
13	28.12014776107875	23.0234825059725	26.944957133839896	21.911412599108854
14	21.78011084744228	22.24432779096122	29.048981146346854	26.926580215249647
15	23.552018664557316	29.11995866524978	25.898271770656056	21.42975089953685
16	21.774118373988937	29.38828830988279	25.913319537327784	22.924273778800494
17	21.662392035603283	28.06075569085229	27.21461843924031	23.062233834304113
18	22.353257641735315	26.868786137944074	29.18414471423891	21.593811506081696
19	24.206396765662326	26.543594578542685	29.310652487142814	19.93935616865217
20	24.230899323782662	26.066593691656614	29.094657110668997	20.607849873891727
21	22.354722468579464	27.701873114035436	28.414444790676246	21.528959626708854
22	21.65653272822668	27.592943263261343	28.74842531114254	22.002098697369437
23	21.773718875758714	27.09037448964101	29.285484098638776	21.8504225359615
24	22.301189705729605	27.433809801555913	28.54641237272652	21.71858811998796
25	22.41025272258044	27.00594719698725	29.032734884984457	21.55106519544785
26	22.133933113342973	27.186254064894495	28.729515728245325	21.95029709351721
27	22.1850688868115	27.258163746334603	28.67212114916998	21.884646217683922
28	21.43534387475997	27.48401341248725	29.079875676150756	22.000767036602028
29	22.559132396376818	27.393194148149924	28.254512332510366	21.793161122962893
30	22.06708374281902	27.615847828460787	28.900101472550478	21.416966956169716
31	22.488554375704116	27.166678651613573	28.722058427947832	21.622708544734483
32	21.1782001805732	27.156025365474296	28.990121740427355	22.675652713525146
33	21.44546449659228	27.109017740384743	29.64756266129741	21.797955101725567
34	21.459313768573338	27.167211315920536	28.722591092254795	22.65088382325133
35	21.869332118858715	27.808272809351454	28.615392400478335	21.7070026713115
36	22.283079119292832	27.59760407594728	28.367969829893653	21.751346974866234
37	22.77153228877863	26.882235911694913	28.076202955754237	22.27002884377222
38	22.412250213731554	27.477621440803684	28.366771335202984	21.743357010261775
39	22.056030958449522	27.174668616218028	28.732978046240586	22.03632237909186
40	22.02953090917807	27.445927914539336	28.699819693132095	21.824721483150498
41	21.91101310087863	27.093437309406056	28.90809143715493	22.087458152560384
42	21.784771660128214	27.187718891738644	28.765603735042124	22.261905713091025
43	21.699811703167487	27.104090595545326	28.698221700211203	22.49787600107598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	62.0
1	69.0
2	76.0
3	313.0
4	550.0
5	550.0
6	703.0
7	856.0
8	842.5
9	829.0
10	1114.0
11	1399.0
12	1399.0
13	2154.5
14	2910.0
15	5526.5
16	8143.0
17	9139.5
18	10136.0
19	10136.0
20	9710.0
21	9284.0
22	10466.5
23	11649.0
24	14102.5
25	16556.0
26	16556.0
27	19148.0
28	21740.0
29	26285.5
30	30831.0
31	32726.5
32	34622.0
33	34622.0
34	37484.0
35	40346.0
36	42048.5
37	43751.0
38	46165.0
39	48579.0
40	48579.0
41	50479.0
42	52379.0
43	51342.0
44	50305.0
45	50762.0
46	51219.0
47	51219.0
48	51023.0
49	50827.0
50	51812.0
51	52797.0
52	53166.0
53	53535.0
54	53535.0
55	48238.0
56	42941.0
57	38489.5
58	34038.0
59	29989.0
60	25940.0
61	25940.0
62	22491.0
63	19042.0
64	15582.0
65	12122.0
66	10202.0
67	8282.0
68	8282.0
69	6822.5
70	5363.0
71	4671.0
72	3979.0
73	3208.5
74	2438.0
75	2438.0
76	1837.0
77	1236.0
78	966.5
79	697.0
80	563.0
81	429.0
82	429.0
83	401.0
84	373.0
85	339.0
86	305.0
87	264.5
88	224.0
89	224.0
90	161.0
91	98.0
92	75.5
93	53.0
94	27.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	750942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.40581807954261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97983750022853	51.385343837060695
2	7.115110159122587	8.311276591392387
3	1.6558674873027992	2.9013688568170997
4	0.7619201210096358	1.7800227191532756
5	0.4318373303163931	1.2610906277207303
6	0.30796886069657303	1.079230395120483
7	0.22392474034844	0.9154955353809863
8	0.16082489762679397	0.7514487770761276
9	0.13074855909321442	0.6872828900904548
>10	0.9798733031063975	11.840458665087175
>50	0.1524087529266303	6.230546354453594
>100	0.09528411774369308	9.890427603564206
>500	0.0034690819566198998	1.3151036334820372
>1k	6.9381639132398E-4	0.953639241206161
>5k	2.3127213044132666E-4	0.6972642723946463
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5162	0.6874032881367669	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3908	0.5204130279036198	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.26819647855626666	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1138	0.15154299533119733	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	973	0.12957059266894114	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	953	0.1269072711341222	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	764	0.10173888263008328	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	753	0.10027405578593286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	1.331660767409467E-4	0.0	0.0
5	0.0	0.0	3.994982302228401E-4	0.0	0.0
6	0.0	0.0	7.989964604456802E-4	0.0	0.0
7	0.0	0.0	0.002263823304596094	1.331660767409467E-4	0.0
8	0.0	0.0	0.0029296536883008273	1.331660767409467E-4	0.0
9	0.0	0.0	0.0037286501487465078	1.331660767409467E-4	0.0
10	2.663321534818934E-4	0.0	0.004128148378969348	2.663321534818934E-4	0.0
11	2.663321534818934E-4	0.0	0.005859307376601655	2.663321534818934E-4	0.0
12	2.663321534818934E-4	0.0	0.0065251377603063885	2.663321534818934E-4	0.0
13	2.663321534818934E-4	0.0	0.0074573002974930155	2.663321534818934E-4	0.0
14	2.663321534818934E-4	0.0	0.008256296757938696	2.663321534818934E-4	0.0
15	2.663321534818934E-4	0.0	0.009321625371866269	3.994982302228401E-4	0.0
16	2.663321534818934E-4	0.0	0.009854289678830057	7.989964604456802E-4	0.0
17	2.663321534818934E-4	0.0	0.009987455755571003	7.989964604456802E-4	0.0
18	2.663321534818934E-4	0.0	0.01012062183231195	9.321625371866269E-4	0.0
19	2.663321534818934E-4	0.0	0.010653286139275736	9.321625371866269E-4	0.0
20	2.663321534818934E-4	0.0	0.011452282599721416	0.0011984946906685202	0.0
21	2.663321534818934E-4	0.0	0.01171861475320331	0.0011984946906685202	0.0
22	3.994982302228401E-4	0.0	0.011984946906685204	0.0021306572278551473	0.0
23	3.994982302228401E-4	0.0	0.012251279060167097	0.0027964876115598807	0.0
24	3.994982302228401E-4	0.0	0.012251279060167097	0.0033291519185236675	0.0
25	3.994982302228401E-4	0.0	0.012384445136908043	0.003595484072005561	0.0
26	3.994982302228401E-4	0.0	0.012384445136908043	0.004128148378969348	0.0
27	3.994982302228401E-4	0.0	0.012384445136908043	0.005726141299860708	0.0
28	3.994982302228401E-4	0.0	0.01251761121364899	0.016246261362395497	0.0
29	3.994982302228401E-4	0.0	0.01251761121364899	0.037819165794428865	0.0
30	3.994982302228401E-4	0.0	0.012650777290389936	0.06924635990529229	0.0
31	3.994982302228401E-4	0.0	0.012650777290389936	0.13862588588732552	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAT	35	8.8665204E-4	26.42857	1
GGTATCA	1005	0.0	23.37811	1
GTATCAA	2545	0.0	22.607073	1
ATCTCGC	110	3.846253E-8	18.5	11
CGAACTA	110	3.846253E-8	18.5	24
CGAGTCG	60	9.232749E-4	18.5	21
GCGAACT	110	3.846253E-8	18.5	23
ACGGCGT	60	9.232749E-4	18.5	35
AGCATCG	50	0.0070326673	18.499998	7
GTATAAC	50	0.0070326673	18.499998	1
TTTACGG	65	0.0015792443	17.076923	32
CGGTACA	65	0.0015792443	17.076923	33
GTTTATA	245	0.0	16.612246	1
GTATTAG	150	4.660251E-9	16.033333	1
TTAGGGC	70	0.0025913715	15.857142	3
AAAGCGA	140	3.4669938E-8	15.857142	20
CGGCAAG	200	3.6379788E-12	15.724999	18
TCTCGCC	130	2.585275E-7	15.653846	12
TGCTCGG	120	1.9332274E-6	15.416667	10
TTGGCCG	145	5.3361873E-8	15.310345	31
>>END_MODULE