FastQCFastQC Report
Fri 10 Feb 2017
ERR1633498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613923
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22170.36112020562839314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.24807671320344732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8500.13845384518905465No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8030.1307981619844834No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7510.12232804439644711No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7080.11532390869864786No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7040.11467236119187586No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6430.10473626171360252No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6230.10147852417974242No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6200.1009898635496634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA358.8646705E-426.4285737
GGTATCA4300.025.8139531
CGCAACT451.3222314E-424.66666615
TCTATAC701.9198887E-723.7857133
ATCGGGC400.001930167423.12500210
GTATCAA12400.021.7822591
TTACGGG450.003823808420.55555529
TAACGGC1550.020.29032336
TTAACGG1550.020.29032335
GCCGGCA759.254247E-619.73333215
CTGCTCG2550.019.5882369
TTTCGGG951.6729427E-719.47368422
AACGGCC1650.019.06060637
ACAGTTC1103.842979E-818.58
TCGGGCG500.007031238618.511
TTGCGGT701.2180237E-418.535
TGCTCGG1800.018.510
CGCAACC500.007031238618.514
TTCGCCG801.6146714E-518.524
GCGTCAA500.007031238618.526
Produced by FastQC (version 0.11.5)