##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633498.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613923
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.656544876148963	33.0	33.0	33.0	27.0	33.0
2	31.815931313861835	33.0	33.0	33.0	27.0	33.0
3	31.544649736204704	33.0	33.0	33.0	27.0	33.0
4	31.849437144397587	33.0	33.0	33.0	27.0	33.0
5	31.945770072142597	33.0	33.0	33.0	33.0	33.0
6	35.05491893934581	37.0	37.0	37.0	33.0	37.0
7	35.199207392458014	37.0	37.0	37.0	33.0	37.0
8	35.33667251430554	37.0	37.0	37.0	33.0	37.0
9	35.45247042381536	37.0	37.0	37.0	33.0	37.0
10	35.462312048905154	37.0	37.0	37.0	33.0	37.0
11	35.47417998674101	37.0	37.0	37.0	33.0	37.0
12	35.48798464954074	37.0	37.0	37.0	33.0	37.0
13	35.484244766851866	37.0	37.0	37.0	33.0	37.0
14	35.466579685074514	37.0	37.0	37.0	33.0	37.0
15	35.46719702633718	37.0	37.0	37.0	33.0	37.0
16	35.44593051571614	37.0	37.0	37.0	33.0	37.0
17	35.443700594374214	37.0	37.0	37.0	33.0	37.0
18	35.423494477320446	37.0	37.0	37.0	33.0	37.0
19	35.41532244271676	37.0	37.0	37.0	33.0	37.0
20	35.42640526580695	37.0	37.0	37.0	33.0	37.0
21	35.42071562720406	37.0	37.0	37.0	33.0	37.0
22	35.3248681023516	37.0	37.0	37.0	33.0	37.0
23	35.363633550135766	37.0	37.0	37.0	33.0	37.0
24	35.35352153283066	37.0	37.0	37.0	33.0	37.0
25	35.372892040207	37.0	37.0	37.0	33.0	37.0
26	35.26348744060737	37.0	37.0	37.0	33.0	37.0
27	35.274610985416736	37.0	37.0	37.0	33.0	37.0
28	35.301071958535516	37.0	37.0	37.0	33.0	37.0
29	35.298337087875844	37.0	37.0	37.0	33.0	37.0
30	35.29094039480521	37.0	37.0	37.0	33.0	37.0
31	35.28704902732102	37.0	37.0	37.0	33.0	37.0
32	35.279518767011496	37.0	37.0	37.0	33.0	37.0
33	35.27092811313471	37.0	37.0	37.0	33.0	37.0
34	35.257991637387754	37.0	37.0	37.0	33.0	37.0
35	35.20353855450928	37.0	37.0	37.0	33.0	37.0
36	35.220190479913605	37.0	37.0	37.0	33.0	37.0
37	35.220561861992465	37.0	37.0	37.0	33.0	37.0
38	35.206003032953646	37.0	37.0	37.0	33.0	37.0
39	35.10361885773949	37.0	37.0	37.0	33.0	37.0
40	34.91522063841882	37.0	37.0	37.0	27.0	37.0
41	35.07640371838162	37.0	37.0	37.0	27.0	37.0
42	35.11775906750521	37.0	37.0	37.0	33.0	37.0
43	34.7689091302981	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	16.0
16	36.0
17	25.0
18	28.0
19	31.0
20	61.0
21	171.0
22	388.0
23	882.0
24	1723.0
25	3039.0
26	4746.0
27	7106.0
28	9778.0
29	13047.0
30	16824.0
31	21245.0
32	26976.0
33	34792.0
34	50598.0
35	100247.0
36	322160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.378353637182514	21.396494348639813	13.702858501799087	24.52229351237859
2	15.850684857873057	21.71884747761527	38.843470598104325	23.58699706640735
3	17.373677154952656	27.78345167064925	30.05800401679038	24.784867157607714
4	12.221565245152894	18.357351003301716	40.279481303029854	29.14160244851553
5	13.374152784632601	37.598851973293065	36.102736010867815	12.92425923120652
6	28.937179418265806	40.744686222865084	17.551223850548034	12.766910508321075
7	26.180319030236692	32.42035238946904	24.139020691519946	17.260307888774324
8	24.178765089433043	36.3032497560769	20.832417094651934	18.685568059838122
9	25.55255300746185	15.523608009473499	20.670996199849167	38.252842783215485
10	14.627893074538663	28.37407948553809	34.515403397494474	22.48262404242877
11	33.508762499531706	23.814875806900865	24.72297014446437	17.953391549103063
12	22.42821982561331	26.13503647851603	30.691959740879554	20.744783954991096
13	27.24348167441194	22.866548410794188	26.37057090221412	23.519399012579754
14	21.464418176220796	21.214549707373724	28.31625464431207	29.00477747209341
15	23.567776414957574	29.39472865489646	24.72997428016217	22.307520649983793
16	22.58263658471828	28.95183923716818	25.544571550503893	22.92095262760965
17	22.079641909490277	28.124699677321097	26.90272232837017	22.892936084818455
18	22.26663604393385	28.304526789190177	28.006932465472055	21.42190470140392
19	24.04992156996887	27.054695784324746	27.724975281916464	21.170407363789923
20	23.39283590938929	27.03270605597119	28.09391405762612	21.480543977013404
21	22.335211419021604	27.550523436978253	28.167213152137972	21.94705199186217
22	22.207508107694288	27.392848940339427	27.87417966096725	22.525463290999035
23	22.2630525326466	27.285017827968655	28.27879066267268	22.173138976712064
24	22.475294132977588	28.155322410139384	26.552352656603517	22.817030800279515
25	22.56097263011811	27.49693365454625	27.84420847565574	22.097885239679897
26	22.507057073932724	27.67822674830557	27.989503569665903	21.825212608095804
27	22.437178603831427	27.670082404470918	27.352452994919556	22.5402859967781
28	21.96154892388785	27.485531573177745	28.366260915456827	22.186658587477584
29	23.630487862484383	27.212370280963572	27.207646561539477	21.949495295012568
30	23.01363526044797	26.991984336797938	27.90219620375845	22.09218419899564
31	22.546801471845818	27.157640290394724	27.95627464682053	22.33928359093893
32	21.639358681789084	27.21057852531995	28.298499974752534	22.851562818138433
33	21.6901793873173	27.149658833436767	28.750511057575622	22.409650721670307
34	22.421378576792204	27.198850670198055	28.218033857666192	22.16173689534355
35	22.556411797570703	27.689954603427463	27.485531573177745	22.268102025824085
36	23.300316163427663	27.374931383903196	27.25716417205415	22.067588280615
37	22.535725164230694	26.905654292150643	27.56257706585353	22.996043477765127
38	22.818822555923138	27.255698190163912	27.49335014325901	22.432129110653943
39	22.14544820767425	27.320852940841117	27.921905515838308	22.611793335646325
40	22.367300133730126	27.956926194327302	27.981359225831255	21.69441444611132
41	22.27657214341212	26.79928916167011	28.00172008541788	22.92241860949989
42	22.032404715249307	27.83492392368424	27.847466213189602	22.28520514787685
43	22.657727434873753	26.843268618377223	27.78882693758012	22.710177009168902
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	30.0
2	37.0
3	126.5
4	216.0
5	216.0
6	294.0
7	372.0
8	356.5
9	341.0
10	435.0
11	529.0
12	529.0
13	891.0
14	1253.0
15	2183.0
16	3113.0
17	3732.5
18	4352.0
19	4352.0
20	4379.5
21	4407.0
22	4992.5
23	5578.0
24	7205.0
25	8832.0
26	8832.0
27	11586.0
28	14340.0
29	16969.0
30	19598.0
31	23016.0
32	26434.0
33	26434.0
34	31533.0
35	36632.0
36	39449.5
37	42267.0
38	45204.0
39	48141.0
40	48141.0
41	49683.5
42	51226.0
43	51552.0
44	51878.0
45	50067.5
46	48257.0
47	48257.0
48	45974.0
49	43691.0
50	42795.5
51	41900.0
52	39537.0
53	37174.0
54	37174.0
55	35504.0
56	33834.0
57	29093.0
58	24352.0
59	22426.5
60	20501.0
61	20501.0
62	18048.0
63	15595.0
64	13022.0
65	10449.0
66	8716.5
67	6984.0
68	6984.0
69	5738.5
70	4493.0
71	3718.5
72	2944.0
73	2344.5
74	1745.0
75	1745.0
76	1365.0
77	985.0
78	771.5
79	558.0
80	457.5
81	357.0
82	357.0
83	297.0
84	237.0
85	200.5
86	164.0
87	123.5
88	83.0
89	83.0
90	61.5
91	40.0
92	24.0
93	8.0
94	5.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	613923.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.33658776422437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.17072907268377	52.5958434516541
2	7.430449916445698	8.966559870225991
3	1.8865701120227545	3.414876094122706
4	0.860565784513442	2.076944119367356
5	0.5143301697214967	1.551646371259475
6	0.3419871299109082	1.2380601886862828
7	0.25024750053993194	1.0569356195373776
8	0.17504060139842947	0.8449082086863164
9	0.1510117095249836	0.8200378138661759
>10	1.0204561699101145	12.224133670529449
>50	0.12211760123886355	5.14353545312529
>100	0.0723963267991318	8.06079775556623
>500	0.0035515179184474055	1.3892356853279224
>1k	5.463873720688316E-4	0.616485698045376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2217	0.36112020562839314	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1523	0.24807671320344732	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.13845384518905465	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	803	0.1307981619844834	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	751	0.12232804439644711	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	708	0.11532390869864786	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	704	0.11467236119187586	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	643	0.10473626171360252	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	623	0.10147852417974242	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	620	0.1009898635496634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.6288687669300546E-4	1.6288687669300546E-4	0.0
4	0.0	0.0	1.6288687669300546E-4	1.6288687669300546E-4	0.0
5	0.0	0.0	3.257737533860109E-4	1.6288687669300546E-4	0.0
6	0.0	0.0	8.144343834650274E-4	1.6288687669300546E-4	0.0
7	0.0	0.0	0.0016288687669300547	1.6288687669300546E-4	0.0
8	0.0	0.0	0.0019546425203160658	1.6288687669300546E-4	0.0
9	0.0	0.0	0.002769076903781093	3.257737533860109E-4	0.0
10	0.0	0.0	0.003094850657167104	4.886606300790164E-4	0.0
11	0.0	0.0	0.004072171917325137	6.515475067720218E-4	0.0
12	0.0	0.0	0.004072171917325137	6.515475067720218E-4	0.0
13	0.0	0.0	0.004560832547404153	6.515475067720218E-4	0.0
14	0.0	0.0	0.005212380054176175	6.515475067720218E-4	0.0
15	0.0	0.0	0.005863927560948197	6.515475067720218E-4	0.0
16	0.0	0.0	0.006515475067720219	6.515475067720218E-4	3.257737533860109E-4
17	0.0	0.0	0.006515475067720219	6.515475067720218E-4	3.257737533860109E-4
18	0.0	0.0	0.0066783619444132246	6.515475067720218E-4	3.257737533860109E-4
19	0.0	0.0	0.0066783619444132246	9.773212601580329E-4	3.257737533860109E-4
20	0.0	0.0	0.007004135697799236	0.0011402081368510383	3.257737533860109E-4
21	0.0	0.0	0.007004135697799236	0.0013030950135440437	3.257737533860109E-4
22	0.0	0.0	0.007004135697799236	0.0014659818902370493	3.257737533860109E-4
23	0.0	0.0	0.007004135697799236	0.0019546425203160658	3.257737533860109E-4
24	0.0	0.0	0.007004135697799236	0.0019546425203160658	3.257737533860109E-4
25	0.0	0.0	0.0071670225744922406	0.003094850657167104	3.257737533860109E-4
26	0.0	0.0	0.0071670225744922406	0.003746398163939126	3.257737533860109E-4
27	1.6288687669300546E-4	0.0	0.0071670225744922406	0.004560832547404153	3.257737533860109E-4
28	1.6288687669300546E-4	0.0	0.0071670225744922406	0.010913420738431366	3.257737533860109E-4
29	1.6288687669300546E-4	0.0	0.0071670225744922406	0.023944370873871804	3.257737533860109E-4
30	1.6288687669300546E-4	0.0	0.0071670225744922406	0.04560832547404153	3.257737533860109E-4
31	1.6288687669300546E-4	0.0	0.0071670225744922406	0.09773212601580328	3.257737533860109E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	35	8.8646705E-4	26.42857	37
GGTATCA	430	0.0	25.813953	1
CGCAACT	45	1.3222314E-4	24.666666	15
TCTATAC	70	1.9198887E-7	23.785713	3
ATCGGGC	40	0.0019301674	23.125002	10
GTATCAA	1240	0.0	21.782259	1
TTACGGG	45	0.0038238084	20.555555	29
TAACGGC	155	0.0	20.290323	36
TTAACGG	155	0.0	20.290323	35
GCCGGCA	75	9.254247E-6	19.733332	15
CTGCTCG	255	0.0	19.588236	9
TTTCGGG	95	1.6729427E-7	19.473684	22
AACGGCC	165	0.0	19.060606	37
ACAGTTC	110	3.842979E-8	18.5	8
TCGGGCG	50	0.0070312386	18.5	11
TTGCGGT	70	1.2180237E-4	18.5	35
TGCTCGG	180	0.0	18.5	10
CGCAACC	50	0.0070312386	18.5	14
TTCGCCG	80	1.6146714E-5	18.5	24
GCGTCAA	50	0.0070312386	18.5	26
>>END_MODULE