FastQCFastQC Report
Fri 10 Feb 2017
ERR1633497.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633497.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395918
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15040.3798766411226567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.29804151364676523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7080.17882490818805916No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4510.11391247682600943No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4480.11315474416419562No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4370.11037639107087831No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4350.10987123596300245No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4010.1012835991291126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG200.001840479237.015
TCGATTG250.00549204529.613
GGTATCA3650.025.8493161
GAGGCAC1103.6379788E-1223.5454547
TACCGAG652.6754751E-622.7692326
GCGACAA603.7183498E-521.58333216
CCTACCG603.7183498E-521.58333224
TATTAGA1301.8189894E-1221.3461532
TCGATAC450.003821446620.5555531
GTATTAG1550.020.2903231
CGGTGAT555.134913E-420.18181830
TTAACGG1202.3646862E-1020.04166635
GTGACAC1202.3646862E-1020.04166624
CGACAAA656.8875306E-519.92307717
GCACCGC1303.274181E-1119.92307710
CTACCGA656.8875306E-519.92307725
CGAGCCT656.8875306E-519.92307729
GTACAGA851.2410928E-619.5882361
TTAGAGG1700.019.5882364
TAACGGC1254.0927262E-1019.2436
Produced by FastQC (version 0.11.5)