##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633497.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395918
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.961726923251785	33.0	33.0	33.0	27.0	33.0
2	31.30392909642906	33.0	33.0	33.0	27.0	33.0
3	31.06300041927874	33.0	33.0	33.0	27.0	33.0
4	31.409875277203867	33.0	33.0	33.0	27.0	33.0
5	31.502629332336493	33.0	33.0	33.0	27.0	33.0
6	34.102809167554895	37.0	33.0	37.0	27.0	37.0
7	34.28218469481054	37.0	33.0	37.0	27.0	37.0
8	34.42169085517708	37.0	37.0	37.0	27.0	37.0
9	34.55174808925081	37.0	37.0	37.0	27.0	37.0
10	34.50137402189342	37.0	37.0	37.0	27.0	37.0
11	34.54240777130618	37.0	37.0	37.0	27.0	37.0
12	34.58729332841649	37.0	37.0	37.0	27.0	37.0
13	34.59093297096874	37.0	37.0	37.0	27.0	37.0
14	34.617635469971056	37.0	37.0	37.0	27.0	37.0
15	34.62683181870994	37.0	37.0	37.0	27.0	37.0
16	34.602508600265715	37.0	37.0	37.0	27.0	37.0
17	34.59741916255386	37.0	37.0	37.0	27.0	37.0
18	34.5499775205977	37.0	37.0	37.0	27.0	37.0
19	34.51420243585793	37.0	37.0	37.0	27.0	37.0
20	34.542887668658665	37.0	37.0	37.0	27.0	37.0
21	34.558597992513604	37.0	37.0	37.0	27.0	37.0
22	34.456804186725535	37.0	37.0	37.0	27.0	37.0
23	34.49273839532428	37.0	37.0	37.0	27.0	37.0
24	34.46333584227037	37.0	37.0	37.0	27.0	37.0
25	34.47820255709516	37.0	37.0	37.0	27.0	37.0
26	34.359781570931354	37.0	37.0	37.0	27.0	37.0
27	34.36053172626655	37.0	37.0	37.0	27.0	37.0
28	34.342876555246285	37.0	37.0	37.0	27.0	37.0
29	34.348951045418495	37.0	37.0	37.0	27.0	37.0
30	34.35388893659798	37.0	37.0	37.0	27.0	37.0
31	34.370278694073015	37.0	37.0	37.0	27.0	37.0
32	34.331502987992465	37.0	37.0	37.0	27.0	37.0
33	34.33715819942513	37.0	37.0	37.0	27.0	37.0
34	34.314289827691596	37.0	37.0	37.0	27.0	37.0
35	34.2442627008623	37.0	37.0	37.0	27.0	37.0
36	34.26587071060169	37.0	37.0	37.0	27.0	37.0
37	34.27002561136397	37.0	37.0	37.0	27.0	37.0
38	34.23437681540117	37.0	37.0	37.0	27.0	37.0
39	34.14699255906526	37.0	37.0	37.0	27.0	37.0
40	33.93206926686839	37.0	33.0	37.0	27.0	37.0
41	34.118648305962346	37.0	37.0	37.0	27.0	37.0
42	34.15147075909658	37.0	37.0	37.0	27.0	37.0
43	33.72411711516021	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	18.0
16	18.0
17	19.0
18	32.0
19	49.0
20	138.0
21	299.0
22	714.0
23	1418.0
24	2587.0
25	4075.0
26	5809.0
27	7909.0
28	10333.0
29	13031.0
30	16212.0
31	19940.0
32	24509.0
33	31792.0
34	44524.0
35	78044.0
36	134447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32375390863765	21.88382442829071	13.512393980571735	24.28002768249991
2	16.358943013452283	22.405649654726485	37.591622507690985	23.643784824130247
3	18.357589197763176	27.614051394480676	29.841027687551463	24.18733172020469
4	13.046135816002302	19.122141453533306	39.01085578326825	28.820866947196137
5	14.349941149429931	37.387539844109135	35.338125571456715	12.924393435004216
6	29.298743679246712	40.4126612076238	17.428103799271565	12.860491313857919
7	26.501952424491943	32.338514540889776	23.61196005233407	17.54757298228421
8	24.224208043079628	36.479018382594376	20.50601387155926	18.790759702766735
9	25.5050288190989	15.54235978157093	20.527735541197924	38.42487585813224
10	14.819482822200555	28.379108805358683	33.97622740062336	22.825180971817396
11	33.75749523891311	23.77083133376103	24.32195555645361	18.14971787087225
12	22.55163948090261	26.157184063366657	30.59648715137983	20.694689304350902
13	27.6701236114549	22.6461034860754	26.302921311988847	23.38085159048086
14	21.430952874079985	21.35972600386949	28.12981475962194	29.07950636242858
15	23.676367328588242	29.304552962987284	24.482089725650262	22.536989982774212
16	22.57159310766371	28.89133608474482	25.402229754646164	23.13484105294531
17	22.126804035179003	28.159113755878746	26.686334038866633	23.027748170075622
18	22.507943564071347	27.855515536045345	27.968922857763477	21.667618042119834
19	24.19566677948464	27.047267363443943	27.57869053692936	21.17837532014205
20	23.693037447148146	26.62344222793609	28.00074762955966	21.68277269535611
21	22.757995342469904	27.393551189892857	27.975237296611926	21.873216171025316
22	22.401608413863478	27.321819164574485	27.74286594698902	22.533706474573016
23	22.47586621472123	27.174566450628667	28.33945412939043	22.010113205259675
24	22.69131486823029	27.81762890295465	26.642132966927495	22.848923261887563
25	22.617562222480412	27.447350208881637	27.9118403305735	22.023247238064446
26	22.75142832606752	27.673659697210027	27.803737137488067	21.771174839234387
27	22.78805207138852	27.398855318525555	27.17961800170742	22.633474608378503
28	22.274561904232694	27.32358720745205	28.167448815158696	22.234402073156563
29	23.699857041104472	27.051056026753013	27.16850458933415	22.08058234280836
30	23.01335124950116	26.99296318934729	27.866628948418615	22.12705661273294
31	22.935809940442212	27.024282806035593	27.59283487994989	22.447072373572304
32	21.885592471168273	27.043983855242754	27.94038159416849	23.130042079420484
33	21.863365646421734	27.332679999393818	28.369763435862982	22.43419091832147
34	22.529665233710013	26.9770508034492	28.108093089983278	22.38519087285751
35	22.55214463601049	27.821417566263722	27.558989487722208	22.067448310003588
36	23.494764067306868	27.156128289191194	27.222808763430812	22.126298880071126
37	22.774412883475872	26.912138372087153	27.34177279133558	22.9716759531014
38	22.76355204865654	27.319293389035103	27.199319050914582	22.717835511393776
39	22.35942796235584	27.132385999121027	27.717860769149166	22.790325269373962
40	22.438484736738413	28.057325001641754	27.61379881692674	21.890391444693094
41	22.44530433069474	26.72371551684945	27.80878868856683	23.022191463888987
42	22.170499952010264	27.818891790724344	27.70497931389833	22.30562894336706
43	22.941114069074906	26.69668971857809	27.502412115640105	22.859784096706896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	17.0
2	22.0
3	72.0
4	122.0
5	122.0
6	190.5
7	259.0
8	236.0
9	213.0
10	291.0
11	369.0
12	369.0
13	614.0
14	859.0
15	1587.0
16	2315.0
17	2679.5
18	3044.0
19	3044.0
20	3022.5
21	3001.0
22	3367.5
23	3734.0
24	4748.5
25	5763.0
26	5763.0
27	7264.0
28	8765.0
29	10480.0
30	12195.0
31	14224.5
32	16254.0
33	16254.0
34	19355.5
35	22457.0
36	24015.5
37	25574.0
38	27400.5
39	29227.0
40	29227.0
41	30407.0
42	31587.0
43	32114.0
44	32641.0
45	32139.0
46	31637.0
47	31637.0
48	30200.5
49	28764.0
50	28408.5
51	28053.0
52	26743.0
53	25433.0
54	25433.0
55	24196.5
56	22960.0
57	20112.0
58	17264.0
59	15718.5
60	14173.0
61	14173.0
62	12323.5
63	10474.0
64	8632.0
65	6790.0
66	5698.0
67	4606.0
68	4606.0
69	3850.0
70	3094.0
71	2472.0
72	1850.0
73	1432.0
74	1014.0
75	1014.0
76	787.0
77	560.0
78	439.0
79	318.0
80	264.0
81	210.0
82	210.0
83	165.0
84	120.0
85	111.0
86	102.0
87	78.0
88	54.0
89	54.0
90	38.0
91	22.0
92	13.0
93	4.0
94	3.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	395918.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.47173153515602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.60597674927723	55.60503037105246
2	7.008810275928176	8.897226484291119
3	1.922390953770629	3.660524475700256
4	0.9078252638354388	2.3048496570798065
5	0.5240641053009585	1.6631628099437084
6	0.36168519060667315	1.3774071191057093
7	0.2612866701285391	1.1609022166079455
8	0.1857208066611582	0.9430416944711719
9	0.13333808596349006	0.7616879276117576
>10	0.9241303296594512	11.427757555392278
>50	0.10675908496418898	4.725940842032514
>100	0.056803890489394354	6.605117297313713
>500	4.028644715559882E-4	0.18103917953227164
>1k	8.057289431119764E-4	0.6863123698652783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1504	0.3798766411226567	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.29804151364676523	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.17882490818805916	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	451	0.11391247682600943	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	448	0.11315474416419562	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	437	0.11037639107087831	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	435	0.10987123596300245	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	401	0.1012835991291126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.525775539379366E-4	0.0	0.0
2	2.525775539379366E-4	0.0	2.525775539379366E-4	0.0	0.0
3	2.525775539379366E-4	0.0	2.525775539379366E-4	0.0	0.0
4	2.525775539379366E-4	0.0	2.525775539379366E-4	0.0	0.0
5	2.525775539379366E-4	0.0	2.525775539379366E-4	0.0	0.0
6	2.525775539379366E-4	0.0	2.525775539379366E-4	0.0	0.0
7	2.525775539379366E-4	0.0	7.577326618138099E-4	2.525775539379366E-4	0.0
8	2.525775539379366E-4	0.0	0.0010103102157517465	2.525775539379366E-4	0.0
9	2.525775539379366E-4	0.0	0.0010103102157517465	0.0010103102157517465	0.0
10	2.525775539379366E-4	0.0	0.0010103102157517465	0.0010103102157517465	0.0
11	2.525775539379366E-4	0.0	0.0025257755393793663	0.0010103102157517465	0.0
12	2.525775539379366E-4	0.0	0.0025257755393793663	0.0010103102157517465	0.0
13	2.525775539379366E-4	0.0	0.0025257755393793663	0.0010103102157517465	0.0
14	2.525775539379366E-4	0.0	0.002778353093317303	0.0010103102157517465	0.0
15	2.525775539379366E-4	0.0	0.002778353093317303	0.0010103102157517465	0.0
16	2.525775539379366E-4	0.0	0.0030309306472552397	0.0010103102157517465	0.0
17	2.525775539379366E-4	0.0	0.0030309306472552397	0.0010103102157517465	0.0
18	2.525775539379366E-4	0.0	0.0030309306472552397	0.0012628877696896832	0.0
19	2.525775539379366E-4	0.0	0.0030309306472552397	0.0012628877696896832	0.0
20	2.525775539379366E-4	0.0	0.0030309306472552397	0.0015154653236276198	0.0
21	2.525775539379366E-4	0.0	0.0030309306472552397	0.0017680428775655565	0.0
22	2.525775539379366E-4	0.0	0.0030309306472552397	0.002020620431503493	0.0
23	2.525775539379366E-4	0.0	0.0030309306472552397	0.0022731979854414297	0.0
24	2.525775539379366E-4	0.0	0.0030309306472552397	0.003536085755131113	0.0
25	2.525775539379366E-4	0.0	0.0030309306472552397	0.0037886633090690497	0.0
26	2.525775539379366E-4	0.0	0.0030309306472552397	0.004041240863006986	0.0
27	2.525775539379366E-4	2.525775539379366E-4	0.0030309306472552397	0.004546395970882859	0.0
28	2.525775539379366E-4	2.525775539379366E-4	0.0030309306472552397	0.01288145525083477	0.0
29	2.525775539379366E-4	2.525775539379366E-4	0.0030309306472552397	0.0255103329477316	0.0
30	2.525775539379366E-4	2.525775539379366E-4	0.0030309306472552397	0.04293818416944923	0.0
31	2.525775539379366E-4	2.525775539379366E-4	0.0030309306472552397	0.08486605812314671	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	20	0.0018404792	37.0	15
TCGATTG	25	0.005492045	29.6	13
GGTATCA	365	0.0	25.849316	1
GAGGCAC	110	3.6379788E-12	23.545454	7
TACCGAG	65	2.6754751E-6	22.76923	26
GCGACAA	60	3.7183498E-5	21.583332	16
CCTACCG	60	3.7183498E-5	21.583332	24
TATTAGA	130	1.8189894E-12	21.346153	2
TCGATAC	45	0.0038214466	20.555553	1
GTATTAG	155	0.0	20.290323	1
CGGTGAT	55	5.134913E-4	20.181818	30
TTAACGG	120	2.3646862E-10	20.041666	35
GTGACAC	120	2.3646862E-10	20.041666	24
CGACAAA	65	6.8875306E-5	19.923077	17
GCACCGC	130	3.274181E-11	19.923077	10
CTACCGA	65	6.8875306E-5	19.923077	25
CGAGCCT	65	6.8875306E-5	19.923077	29
GTACAGA	85	1.2410928E-6	19.588236	1
TTAGAGG	170	0.0	19.588236	4
TAACGGC	125	4.0927262E-10	19.24	36
>>END_MODULE