FastQCFastQC Report
Fri 10 Feb 2017
ERR1633496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628761
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45810.7285757227308945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33450.5319986449541241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18610.2959789172674514No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8780.1396397041165085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.13932161823013833No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7220.11482900497963455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG1000.025.90000224
CCGGCAG1100.025.22727216
GGTATCA7850.024.5095541
GTATCAA22250.023.9460681
GCCGCTC1103.6379788E-1223.54545427
TGTTACG551.9008825E-523.54545416
ATAGAAC400.001930219223.1250023
GCCGGCA1155.456968E-1222.52173815
CGCTCTC1155.456968E-1222.52173829
CGAGTCG753.7351856E-722.20000321
CCGCTCT1201.0913936E-1121.58333428
TAGGACC1450.020.4137944
TGCTCCG1101.7498678E-920.18181828
TGCGGGT851.2434721E-619.58823621
GCTCTCT1457.2759576E-1219.13793230
CTTCGGT801.6147727E-518.52
ACCGCAA701.2180829E-418.58
GCGCACG500.007031425418.59
GCTTCGC1409.276846E-1118.522
GCGGGTA902.1490614E-618.522
Produced by FastQC (version 0.11.5)