##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633496.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628761
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35909351884102	33.0	33.0	33.0	27.0	33.0
2	31.60433773723243	33.0	33.0	33.0	27.0	33.0
3	31.34123458675077	33.0	33.0	33.0	27.0	33.0
4	31.64945981064347	33.0	33.0	33.0	27.0	33.0
5	31.76446376286061	33.0	33.0	33.0	27.0	33.0
6	34.769796472745604	37.0	37.0	37.0	27.0	37.0
7	34.94596993134116	37.0	37.0	37.0	27.0	37.0
8	35.09270613158259	37.0	37.0	37.0	33.0	37.0
9	35.21525508102443	37.0	37.0	37.0	33.0	37.0
10	35.209220991759985	37.0	37.0	37.0	33.0	37.0
11	35.21746736836413	37.0	37.0	37.0	33.0	37.0
12	35.2313025139918	37.0	37.0	37.0	33.0	37.0
13	35.219181851291665	37.0	37.0	37.0	33.0	37.0
14	35.203280737832024	37.0	37.0	37.0	33.0	37.0
15	35.224907397246334	37.0	37.0	37.0	33.0	37.0
16	35.20509382738433	37.0	37.0	37.0	33.0	37.0
17	35.21573857157171	37.0	37.0	37.0	33.0	37.0
18	35.189579188276625	37.0	37.0	37.0	33.0	37.0
19	35.161261592242525	37.0	37.0	37.0	33.0	37.0
20	35.1747722902661	37.0	37.0	37.0	33.0	37.0
21	35.17534325443213	37.0	37.0	37.0	33.0	37.0
22	35.05783914714812	37.0	37.0	37.0	33.0	37.0
23	35.0996722124941	37.0	37.0	37.0	33.0	37.0
24	35.10144554131061	37.0	37.0	37.0	33.0	37.0
25	35.11444571148656	37.0	37.0	37.0	33.0	37.0
26	34.98840417901238	37.0	37.0	37.0	27.0	37.0
27	34.996461294514134	37.0	37.0	37.0	27.0	37.0
28	35.01694602559637	37.0	37.0	37.0	27.0	37.0
29	35.01648480106113	37.0	37.0	37.0	27.0	37.0
30	35.01727842534763	37.0	37.0	37.0	27.0	37.0
31	35.02057220470099	37.0	37.0	37.0	27.0	37.0
32	35.00541382178602	37.0	37.0	37.0	27.0	37.0
33	34.97965999799606	37.0	37.0	37.0	27.0	37.0
34	34.9410682278322	37.0	37.0	37.0	27.0	37.0
35	34.87138356227565	37.0	37.0	37.0	27.0	37.0
36	34.89377839910554	37.0	37.0	37.0	27.0	37.0
37	34.88619046028618	37.0	37.0	37.0	27.0	37.0
38	34.86304971205275	37.0	37.0	37.0	27.0	37.0
39	34.753376879291174	37.0	37.0	37.0	27.0	37.0
40	34.51085229522823	37.0	37.0	37.0	27.0	37.0
41	34.70056507957714	37.0	37.0	37.0	27.0	37.0
42	34.736593077496856	37.0	37.0	37.0	27.0	37.0
43	34.3393785556038	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	18.0
16	25.0
17	33.0
18	30.0
19	40.0
20	78.0
21	213.0
22	555.0
23	1232.0
24	2375.0
25	4004.0
26	6210.0
27	8889.0
28	12079.0
29	15707.0
30	20083.0
31	25359.0
32	31446.0
33	40934.0
34	57901.0
35	110905.0
36	290639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.503369324751375	20.970925359556333	14.094067539176253	23.431637776516038
2	15.8930022695428	22.79451174611657	38.86643732674259	22.446048657598038
3	17.880561930526863	28.00269100659869	30.909041750363013	23.207705312511433
4	12.136885080340543	18.618044058076123	40.568673947652606	28.67639691393073
5	13.60055728647292	37.39560818816688	35.263637534770766	13.740196990589428
6	27.779235671423642	41.34194073741851	17.617027773669168	13.26179581748868
7	25.411245290340844	33.38406803220938	23.554737014541296	17.649949662908483
8	24.494680808765175	35.29290143631682	21.088458094570115	19.123959660347893
9	26.13743536892396	15.240767159540747	20.898242734520746	37.72355473701454
10	14.686661545483895	28.459462339426267	33.96187104480081	22.892005070289027
11	32.850797043709775	24.57833739688053	24.644340218302343	17.926525341107354
12	22.706879084421587	26.43420950090734	31.003036129785404	19.85587528488567
13	28.272745924126973	23.074427326122326	26.594842873524282	22.05798387622642
14	21.780294897425254	21.946812858940042	29.283463828068218	26.989428415566486
15	23.433387248891073	29.14764115458815	25.6943417292103	21.724629867310473
16	21.518510212942594	29.29698247823895	26.28359583371106	22.900911475107392
17	21.541253353818064	28.000782491280468	27.340913320005534	23.11705083489593
18	22.7685877463774	26.880006870655144	29.042036640313253	21.309368742654204
19	24.373967214887692	26.45218135348725	28.969194972334478	20.204656459290575
20	24.330230405511795	26.19166901255008	28.702638999556267	20.77546158238186
21	22.691610961875817	27.026962550158167	28.844346261934184	21.43708022603183
22	21.98116613466802	27.14210964102417	29.14986775579274	21.726856468515063
23	21.998342772532013	26.807483288562743	29.445528587173825	21.748645351731422
24	21.795881105857394	27.08803504034124	28.973330088857292	22.14275376494407
25	22.238020487911943	27.39069376122247	29.014999339971787	21.356286410893805
26	22.320086646595446	27.322623381539252	28.777707268739633	21.57958270312567
27	22.313088757095304	27.140678254535505	28.814923317444947	21.731309670924244
28	21.9323399511102	27.12636438964885	28.93515978249287	22.006135876748083
29	22.42489594615442	27.328826056323468	28.570156227883093	21.67612176963902
30	22.599684140714835	26.991336930884707	28.972693917084552	21.436285011315906
31	22.46767849787121	27.053999850499633	28.68657566229458	21.79174598933458
32	21.294736791881174	27.083104709102507	29.188833276873087	22.43332522214323
33	21.241457405914172	27.29479086648186	29.633199260132226	21.83055246747174
34	21.79572206291421	26.974160293020716	28.981918407789287	22.248199236275788
35	21.846615804733435	27.526516434702536	28.71981563742026	21.90705212314377
36	22.257741812866893	27.18457410685459	28.813014802126723	21.744669278151793
37	22.596185195964765	26.85789990155242	28.5636354672125	21.982279435270318
38	22.405333664142653	27.109028708841677	28.508447565927277	21.977190061088393
39	21.989118281827277	27.03380139671513	28.858978212707214	22.118102108750385
40	21.732900100356098	27.325168068630212	29.108198504678246	21.833733326335445
41	21.973850159281508	26.684225007594303	29.0223153153583	22.319609517765894
42	21.60073541456929	27.311967504345848	28.984781180766618	22.102515900318245
43	22.008998649725413	26.839291877199763	28.90653205271955	22.24517742035527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	50.5
2	64.0
3	252.5
4	441.0
5	441.0
6	578.0
7	715.0
8	679.0
9	643.0
10	902.5
11	1162.0
12	1162.0
13	1866.5
14	2571.0
15	4551.5
16	6532.0
17	7357.0
18	8182.0
19	8182.0
20	7944.5
21	7707.0
22	8445.5
23	9184.0
24	11424.5
25	13665.0
26	13665.0
27	16066.5
28	18468.0
29	20971.0
30	23474.0
31	26227.0
32	28980.0
33	28980.0
34	31984.0
35	34988.0
36	36815.0
37	38642.0
38	39731.0
39	40820.0
40	40820.0
41	41867.0
42	42914.0
43	43375.0
44	43836.0
45	43699.5
46	43563.0
47	43563.0
48	43358.5
49	43154.0
50	42867.5
51	42581.0
52	42019.5
53	41458.0
54	41458.0
55	38714.0
56	35970.0
57	32198.5
58	28427.0
59	25642.0
60	22857.0
61	22857.0
62	20049.5
63	17242.0
64	14106.0
65	10970.0
66	9176.5
67	7383.0
68	7383.0
69	6090.5
70	4798.0
71	4140.5
72	3483.0
73	2600.5
74	1718.0
75	1718.0
76	1259.0
77	800.0
78	614.0
79	428.0
80	360.0
81	292.0
82	292.0
83	246.0
84	200.0
85	190.5
86	181.0
87	155.0
88	129.0
89	129.0
90	103.0
91	77.0
92	49.0
93	21.0
94	12.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	628761.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.90385677308862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.20496009869335	57.89745953694679
2	6.488119419438538	8.422079470518677
3	1.4609969278012753	2.8447300604380943
4	0.6604459164661092	1.714619486747504
5	0.4105730475038654	1.3323887135040697
6	0.2647672372746159	1.0310648907766826
7	0.18750900151742575	0.85190401647063
8	0.15891225492413674	0.8251214586467773
9	0.11482435529948488	0.6707289159378006
>10	0.8919349162968899	11.511232083111397
>50	0.10116651310425137	4.455771914292235
>100	0.05405461309446833	6.376350867521066
>500	9.91827763200047E-4	0.491491268503967
>1k	7.438708224000353E-4	1.5750573165842587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4581	0.7285757227308945	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3345	0.5319986449541241	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1861	0.2959789172674514	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	878	0.1396397041165085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	876	0.13932161823013833	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	722	0.11482900497963455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.180858863701788E-4	0.0	0.0
2	0.0	0.0	3.180858863701788E-4	0.0	0.0
3	0.0	0.0	3.180858863701788E-4	0.0	0.0
4	0.0	0.0	6.361717727403576E-4	0.0	0.0
5	1.590429431850894E-4	0.0	9.542576591105365E-4	0.0	0.0
6	1.590429431850894E-4	0.0	0.0014313864886658046	0.0	0.0
7	1.590429431850894E-4	0.0	0.003498944750071967	0.0	0.0
8	4.7712882955526823E-4	0.0	0.004453202409182504	0.0	0.0
9	4.7712882955526823E-4	0.0	0.00540746006829304	9.542576591105365E-4	0.0
10	6.361717727403576E-4	0.0	0.006679803613773755	9.542576591105365E-4	0.0
11	6.361717727403576E-4	0.0	0.009860662477475543	0.0014313864886658046	0.0
12	6.361717727403576E-4	0.0	0.011451091909326437	0.0014313864886658046	0.0
13	6.361717727403576E-4	0.0	0.012882478397992243	0.0014313864886658046	0.0
14	6.361717727403576E-4	0.0	0.0135186501707326	0.001590429431850894	0.0
15	6.361717727403576E-4	0.0	0.014950036659398404	0.001590429431850894	0.0
16	6.361717727403576E-4	0.0	0.01590429431850894	0.0017494723750359836	0.0
17	6.361717727403576E-4	0.0	0.01606333726169403	0.0017494723750359836	0.0
18	6.361717727403576E-4	0.0	0.01606333726169403	0.001908515318221073	0.0
19	6.361717727403576E-4	0.0	0.01622238020487912	0.001908515318221073	0.0
20	6.361717727403576E-4	0.0	0.016858551977619478	0.002226601204591252	0.0
21	6.361717727403576E-4	0.0	0.017494723750359834	0.0025446870909614306	0.0
22	6.361717727403576E-4	0.0	0.017971852579915102	0.00270373003414652	0.0
23	7.95214715925447E-4	0.0	0.018289938466285283	0.0033399018068868773	0.0
24	7.95214715925447E-4	0.0	0.018289938466285283	0.003817030636442146	0.0
25	9.542576591105365E-4	0.0	0.018289938466285283	0.004294159465997414	0.0
26	9.542576591105365E-4	0.0	0.018289938466285283	0.00524841712510795	0.0
27	9.542576591105365E-4	0.0	0.018289938466285283	0.007315975386514113	0.0
28	9.542576591105365E-4	0.0	0.018289938466285283	0.017335680807174746	0.0
29	9.542576591105365E-4	0.0	0.018289938466285283	0.041669251114493426	0.0
30	0.001113300602295626	0.0	0.018289938466285283	0.07300071092195604	0.0
31	0.001113300602295626	0.0	0.01844898140947037	0.13614075936643652	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCCG	100	0.0	25.900002	24
CCGGCAG	110	0.0	25.227272	16
GGTATCA	785	0.0	24.509554	1
GTATCAA	2225	0.0	23.946068	1
GCCGCTC	110	3.6379788E-12	23.545454	27
TGTTACG	55	1.9008825E-5	23.545454	16
ATAGAAC	40	0.0019302192	23.125002	3
GCCGGCA	115	5.456968E-12	22.521738	15
CGCTCTC	115	5.456968E-12	22.521738	29
CGAGTCG	75	3.7351856E-7	22.200003	21
CCGCTCT	120	1.0913936E-11	21.583334	28
TAGGACC	145	0.0	20.413794	4
TGCTCCG	110	1.7498678E-9	20.181818	28
TGCGGGT	85	1.2434721E-6	19.588236	21
GCTCTCT	145	7.2759576E-12	19.137932	30
CTTCGGT	80	1.6147727E-5	18.5	2
ACCGCAA	70	1.2180829E-4	18.5	8
GCGCACG	50	0.0070314254	18.5	9
GCTTCGC	140	9.276846E-11	18.5	22
GCGGGTA	90	2.1490614E-6	18.5	22
>>END_MODULE