FastQCFastQC Report
Fri 10 Feb 2017
ERR1633495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597949
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41820.6993907507161983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.49669787891609485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16870.28213108475806464No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13780.2304544367496225No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12410.2075427837491157No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11190.18713970589464987No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10190.17041587158771065No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9830.16439529123721253No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8700.1454973584703712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.13629924960155465No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8050.13462686617086073No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7890.13195105268175045No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7690.12860628582036263No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7120.11907370026540726No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7000.11706684014857455No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6920.11572893340401941No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6710.11221692819956217No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6690.1118824515134234No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6410.1071997779074804No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6240.10435672607530073No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6150.1028515809876762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG200.001841229637.018
GAACGGT251.2329176E-436.99999620
GGTATCA7000.030.6571451
ACGGTAA250.005494261629.59999822
CGGGTAT405.935435E-527.75000217
CGCTCTC951.0913936E-1125.3157929
GCCGTCC451.3221751E-424.66666627
CGAGTCG400.001930109123.12500221
TCGGCGT400.001930109123.12500211
GTCGCCC2000.023.12499837
GTATCAA19550.022.8056261
ATTATCC603.722438E-521.5833323
CCGCTCT1059.804353E-1021.14285928
GCCGCTC1059.804353E-1021.14285927
GTACCGG450.003823693620.5555556
TTAAGGT450.003823693620.5555554
CGGGCTA450.003823693620.55555517
GACTGAG450.003823693620.5555557
AAGTCGA450.003823693620.55555515
AGGTCGC2250.020.55555535
Produced by FastQC (version 0.11.5)