FastQCFastQC Report
Fri 10 Feb 2017
ERR1633494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769771
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54400.7067036820041286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.49105513198080986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20040.26033716520887384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.14082110134052855No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9310.12094506028416244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.026.8895341
GTATCAA25250.023.4455451
CTAGTAC753.7374411E-722.23
GTAGACT502.7009792E-422.1999994
AACGGCC759.2592545E-619.73333437
CGCTCTC1052.2564564E-819.3809529
TCGATCA500.007032823718.49999834
GCGTCTC500.007032823718.4999989
TAACGGC953.603258E-617.52631636
TTCGCCG1301.3909812E-817.07692324
GCCGCTC1201.0398435E-716.95833427
GCCCCGT2100.016.73809414
CCGTACA2100.016.73809417
GTCTAGA1005.8727182E-616.651
ACGGTAC1005.8727182E-616.653
CCGCTCT1352.215711E-816.44444528
TGCTCGC1352.215711E-816.44444510
ATCAACG36300.016.3595033
TAGTACT803.3807493E-416.18754
TATCAAC36600.016.1243172
Produced by FastQC (version 0.11.5)