##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633494.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769771
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85916071143236	33.0	33.0	33.0	33.0	33.0
2	31.97616693795947	33.0	33.0	33.0	33.0	33.0
3	31.6996600287618	33.0	33.0	33.0	27.0	33.0
4	31.975422560735595	33.0	33.0	33.0	33.0	33.0
5	32.08020826973217	33.0	33.0	33.0	33.0	33.0
6	35.249496278763424	37.0	37.0	37.0	33.0	37.0
7	35.3912735086149	37.0	37.0	37.0	33.0	37.0
8	35.52628508998131	37.0	37.0	37.0	33.0	37.0
9	35.6327687065374	37.0	37.0	37.0	33.0	37.0
10	35.64941131843106	37.0	37.0	37.0	33.0	37.0
11	35.67892399168064	37.0	37.0	37.0	33.0	37.0
12	35.6677427442707	37.0	37.0	37.0	33.0	37.0
13	35.66141748649923	37.0	37.0	37.0	33.0	37.0
14	35.638537954794344	37.0	37.0	37.0	33.0	37.0
15	35.656125003410104	37.0	37.0	37.0	33.0	37.0
16	35.63472902980237	37.0	37.0	37.0	33.0	37.0
17	35.640213777863806	37.0	37.0	37.0	33.0	37.0
18	35.6122184389903	37.0	37.0	37.0	33.0	37.0
19	35.60910972224207	37.0	37.0	37.0	33.0	37.0
20	35.61810850239876	37.0	37.0	37.0	33.0	37.0
21	35.6024363089802	37.0	37.0	37.0	33.0	37.0
22	35.51273820395936	37.0	37.0	37.0	33.0	37.0
23	35.56353902654166	37.0	37.0	37.0	33.0	37.0
24	35.54815003423096	37.0	37.0	37.0	33.0	37.0
25	35.57088017085601	37.0	37.0	37.0	33.0	37.0
26	35.4551002830712	37.0	37.0	37.0	33.0	37.0
27	35.47038404928219	37.0	37.0	37.0	33.0	37.0
28	35.47723933481516	37.0	37.0	37.0	33.0	37.0
29	35.48802046322867	37.0	37.0	37.0	33.0	37.0
30	35.493438957819926	37.0	37.0	37.0	33.0	37.0
31	35.481614662022864	37.0	37.0	37.0	33.0	37.0
32	35.47462946772482	37.0	37.0	37.0	33.0	37.0
33	35.459692557916576	37.0	37.0	37.0	33.0	37.0
34	35.44005424990029	37.0	37.0	37.0	33.0	37.0
35	35.37246531760744	37.0	37.0	37.0	33.0	37.0
36	35.393097427676544	37.0	37.0	37.0	33.0	37.0
37	35.38209935162536	37.0	37.0	37.0	33.0	37.0
38	35.36884216214952	37.0	37.0	37.0	33.0	37.0
39	35.25743890066007	37.0	37.0	37.0	33.0	37.0
40	35.042339864713014	37.0	37.0	37.0	27.0	37.0
41	35.207985231971584	37.0	37.0	37.0	33.0	37.0
42	35.25133188961392	37.0	37.0	37.0	33.0	37.0
43	34.90192407871952	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	22.0
16	43.0
17	31.0
18	26.0
19	34.0
20	52.0
21	128.0
22	331.0
23	800.0
24	1688.0
25	3114.0
26	5043.0
27	7505.0
28	10589.0
29	14184.0
30	18952.0
31	24071.0
32	31151.0
33	40614.0
34	58495.0
35	118564.0
36	434332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.21823243536065	21.09796290065487	14.16797982776696	23.515824836217526
2	15.477330270950713	22.553460704547195	39.948763983054704	22.020445041447392
3	17.64329911103432	28.18994220359042	30.876455465326703	23.29030322004856
4	12.06748500528079	18.21853512278327	40.83578103098194	28.878198840954
5	13.141960401210229	37.733040085947636	35.59889889330723	13.526100619534901
6	27.663162161214178	41.116124146012254	17.944427628476518	13.276286064297043
7	25.096684598406537	33.368885032041995	23.979079492472437	17.555350877079025
8	23.9307534318648	35.67047862286316	21.49938098473442	18.899386960537615
9	25.898351587679976	15.532801313637432	20.838015461741218	37.730831636941375
10	14.395060349116815	28.434300590695155	34.49389493758533	22.676744122602695
11	32.38820376449619	24.770873415600224	24.90883652410912	17.932086295794463
12	22.23882167553727	26.45059374801077	31.366081600891693	19.944502975560262
13	27.72928572263699	23.231584458234984	27.06311357533604	21.976016243791985
14	21.80492640018915	21.958348651741883	29.138016371102577	27.098708576966395
15	23.22976573552394	29.165816846828474	25.911212555422324	21.69320486222526
16	21.564075549741414	29.494356113701347	26.287818065372687	22.65375027118455
17	21.43000970418475	28.026516977126963	27.514806351499345	23.028666967188943
18	22.287407553675052	27.234723053999176	29.149448342429114	21.328421049896658
19	23.821500160437324	26.74080992918673	29.330930887237894	20.10675902313805
20	23.883856367672983	26.378234565864396	29.05487476145503	20.683034305007595
21	22.26792123891391	27.510129635956666	28.70048365033237	21.52146547479705
22	21.618507322307543	27.429716110375686	28.901972144962595	22.04980442235418
23	21.64747697691911	27.253429916169875	29.453175035172798	21.64591807173822
24	21.93405571267299	27.423740307182264	28.82974287158129	21.812461108563454
25	22.16308486549896	27.297598896295135	29.19452668390989	21.344789554296018
26	22.07968343832127	27.428417022724936	28.88924108598531	21.60265845296848
27	22.16334468302911	27.458425947457098	28.545632402363818	21.83259696714997
28	21.54640795769131	27.514936260264417	29.104370000948332	21.834285781095936
29	22.362105093592767	27.46777937854245	28.486264096724874	21.68385143113991
30	22.074746905248443	27.52805704553692	29.116581424865316	21.28061462434932
31	22.279483119005523	27.238620316951405	28.840655207847526	21.641241356195547
32	21.18097460153734	27.19185316152466	29.18244516875798	22.44472706818002
33	21.38817908183083	27.197699055953006	29.691817436614265	21.722304425601898
34	21.622274676494698	27.085977517989118	29.11801042128113	22.17373738423505
35	21.782452183831293	27.612497742835206	28.95276647210664	21.652283601226856
36	22.21725682053494	27.389184575672505	28.831561594292328	21.561997009500228
37	22.416796683689043	27.084028886513	28.449499916208847	22.049674513589107
38	22.19971913724991	27.34345669036636	28.584475123121035	21.872349049262702
39	21.807914301785853	27.141968195736133	28.91769110553658	22.13242639694143
40	21.70294801960583	27.57430456590337	28.98667265979103	21.736074754699775
41	21.816618189045833	26.929567364839674	29.177898361980382	22.07591608413411
42	21.6086342561619	27.485187153062405	28.87014449751939	22.03603409325631
43	21.748545996146905	26.995301199967265	28.91067603222257	22.34547677166326
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	88.0
2	112.0
3	378.5
4	645.0
5	645.0
6	766.5
7	888.0
8	830.0
9	772.0
10	1067.0
11	1362.0
12	1362.0
13	2187.0
14	3012.0
15	5412.0
16	7812.0
17	9075.0
18	10338.0
19	10338.0
20	9820.5
21	9303.0
22	10529.0
23	11755.0
24	14422.5
25	17090.0
26	17090.0
27	19848.5
28	22607.0
29	26599.0
30	30591.0
31	33475.0
32	36359.0
33	36359.0
34	39783.0
35	43207.0
36	45094.5
37	46982.0
38	49205.0
39	51428.0
40	51428.0
41	52620.0
42	53812.0
43	53530.0
44	53248.0
45	53413.5
46	53579.0
47	53579.0
48	53024.0
49	52469.0
50	52700.0
51	52931.0
52	52923.5
53	52916.0
54	52916.0
55	48600.0
56	44284.0
57	39435.0
58	34586.0
59	30157.5
60	25729.0
61	25729.0
62	22512.5
63	19296.0
64	15530.5
65	11765.0
66	9867.5
67	7970.0
68	7970.0
69	6386.0
70	4802.0
71	4135.0
72	3468.0
73	2789.5
74	2111.0
75	2111.0
76	1567.5
77	1024.0
78	741.5
79	459.0
80	400.5
81	342.0
82	342.0
83	290.5
84	239.0
85	221.0
86	203.0
87	164.5
88	126.0
89	126.0
90	93.0
91	60.0
92	41.5
93	23.0
94	12.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	769771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.672625460272876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.06403560058823	54.311402841152145
2	7.220662638214617	8.90634444923192
3	1.6501708987866348	3.0531111535893
4	0.7154563766965016	1.7649629261266901
5	0.41809249107816826	1.2892430805008177
6	0.27944013785154054	1.034028417617105
7	0.21451547852789687	0.9260812933877536
8	0.16225080042112355	0.800514627600115
9	0.12462324638566709	0.6917258518187898
>10	0.9537518516103014	12.13540022256702
>50	0.12757148420078035	5.384944087306886
>100	0.06708629946704385	7.500362442196461
>500	0.0014908066545709366	0.5852762510360792
>1k	6.389171376732586E-4	0.9020819775843341
>5k	2.129723792244195E-4	0.714520378284621
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5440	0.7067036820041286	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3780	0.49105513198080986	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2004	0.26033716520887384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1084	0.14082110134052855	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	931	0.12094506028416244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.2990876507428832E-4	0.0	0.0
2	0.0	0.0	2.5981753014857665E-4	0.0	0.0
3	0.0	0.0	3.89726295222865E-4	0.0	0.0
4	0.0	0.0	3.89726295222865E-4	2.5981753014857665E-4	0.0
5	0.0	0.0	5.196350602971533E-4	2.5981753014857665E-4	0.0
6	0.0	0.0	9.093613555200183E-4	2.5981753014857665E-4	0.0
7	0.0	0.0	0.0022084490062629016	2.5981753014857665E-4	0.0
8	1.2990876507428832E-4	0.0	0.0027280840665600548	3.89726295222865E-4	0.0
9	1.2990876507428832E-4	0.0	0.0035075366570057847	0.0010392701205943066	0.0
10	1.2990876507428832E-4	0.0	0.00389726295222865	0.0010392701205943066	0.0
11	1.2990876507428832E-4	0.0	0.005326259368045821	0.001169178885668595	0.0
12	1.2990876507428832E-4	0.0	0.005715985663268686	0.001169178885668595	0.0
13	1.2990876507428832E-4	0.0	0.005715985663268686	0.001169178885668595	0.0
14	1.2990876507428832E-4	0.0	0.0061057119584915515	0.001169178885668595	0.0
15	1.2990876507428832E-4	0.0	0.006885164548937281	0.001169178885668595	0.0
16	1.2990876507428832E-4	0.0	0.007274890844160146	0.0012990876507428832	0.0
17	1.2990876507428832E-4	0.0	0.007404799609234435	0.0012990876507428832	0.0
18	2.5981753014857665E-4	0.0	0.0076646171393830115	0.001948631476114325	0.0
19	2.5981753014857665E-4	0.0	0.007924434669531587	0.002078540241188613	0.0
20	3.89726295222865E-4	0.0	0.008184252199680164	0.0022084490062629016	0.0
21	5.196350602971533E-4	0.0	0.008314160964754453	0.0025981753014857664	0.0
22	5.196350602971533E-4	0.0	0.008444069729828741	0.002857992831634343	0.0
23	5.196350602971533E-4	0.0	0.00857397849490303	0.0032477191268572084	0.0
24	5.196350602971533E-4	0.0	0.008703887259977318	0.0033776278919314963	0.0
25	5.196350602971533E-4	0.0	0.008703887259977318	0.003637445422080073	0.0
26	5.196350602971533E-4	0.0	0.008703887259977318	0.004416898012525803	0.0
27	5.196350602971533E-4	0.0	0.008703887259977318	0.00623562072356584	0.0
28	5.196350602971533E-4	0.0	0.008703887259977318	0.015329234278766023	0.0
29	5.196350602971533E-4	0.0	0.008703887259977318	0.04196053111899513	0.0
30	5.196350602971533E-4	0.0	0.008703887259977318	0.07313863473682433	0.0
31	5.196350602971533E-4	0.0	0.008703887259977318	0.1436790941721629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	860	0.0	26.889534	1
GTATCAA	2525	0.0	23.445545	1
CTAGTAC	75	3.7374411E-7	22.2	3
GTAGACT	50	2.7009792E-4	22.199999	4
AACGGCC	75	9.2592545E-6	19.733334	37
CGCTCTC	105	2.2564564E-8	19.38095	29
TCGATCA	50	0.0070328237	18.499998	34
GCGTCTC	50	0.0070328237	18.499998	9
TAACGGC	95	3.603258E-6	17.526316	36
TTCGCCG	130	1.3909812E-8	17.076923	24
GCCGCTC	120	1.0398435E-7	16.958334	27
GCCCCGT	210	0.0	16.738094	14
CCGTACA	210	0.0	16.738094	17
GTCTAGA	100	5.8727182E-6	16.65	1
ACGGTAC	100	5.8727182E-6	16.65	3
CCGCTCT	135	2.215711E-8	16.444445	28
TGCTCGC	135	2.215711E-8	16.444445	10
ATCAACG	3630	0.0	16.359503	3
TAGTACT	80	3.3807493E-4	16.1875	4
TATCAAC	3660	0.0	16.124317	2
>>END_MODULE