FastQCFastQC Report
Fri 10 Feb 2017
ERR1633493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798701
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41840.5238506024156725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.3782391658455417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16270.20370576723955522No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9160.11468622175257073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.10179028197034934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.024.3446461
GGTATCA6500.024.1923081
CCTACGT806.9546513E-720.812534
TTCGCCG450.003824800720.55555724
TACGTGA759.259968E-619.73333436
GCTATAC609.233453E-418.53
AATCGTG500.0070330518.4999987
CGTGACG1054.792728E-717.6190536
TCGTGAC1054.792728E-717.6190535
ACGGGTC953.6036E-617.52631616
CGCAATA852.721431E-517.41176428
CGGGTCA650.001579363417.07692317
TCGTTGG650.001579363417.07692322
ACCGACC3050.016.9836068
CCCGCTG1107.800263E-716.81818225
AACGATT1005.873275E-616.6522
AGCTTCG904.4432236E-516.44444521
ACGTGAT904.4432236E-516.44444537
TAAGTCT1151.2409564E-616.0869565
CCGACCT3250.015.9384629
Produced by FastQC (version 0.11.5)