FastQCFastQC Report
Fri 10 Feb 2017
ERR1633492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680814
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32420.47619467284750316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.33724335868533845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12820.1883040007990435No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8600.12631937651105882No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8530.1252911955394572No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7990.11735951375853022No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7550.1108966619370342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG250.00549478929.64
GGTATCA6050.027.2148761
TCCGTGC551.9011823E-523.5454548
GTATCAA17450.023.1117481
GTATTGC555.140135E-420.18181814
AGCGCAC656.896848E-519.92307733
ATGCGTT759.256672E-619.73333436
TGCGTTA759.256672E-619.73333437
CATAATA2150.018.9302332
CGGGTCA902.1495944E-618.517
TAATACG609.231542E-418.527
TAAAGCG500.007032008418.530
CGGTTGG1258.560164E-917.7633
GTCTAGA953.6020192E-617.5263161
TTCAACG852.7204747E-517.41176619
CGATTAA852.7204747E-517.41176624
CTACACT852.7204747E-517.4117664
GGTCAAC752.0657065E-417.26666619
CTGCTCG2550.016.6862749
AATGCGT1005.870712E-616.65000235
Produced by FastQC (version 0.11.5)