FastQCFastQC Report
Fri 10 Feb 2017
ERR1633491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701559
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80691.150152731274205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59710.851104468761715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29700.4233428692383677No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA21190.30204159593134716No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG19380.27624191265453085No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC18830.2684022298908574No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC18580.2648387377255512No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16480.2349054035369798No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA16460.2346203241637553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.22820603826620425No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG15460.2203663555025308No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14490.20654000590114302No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG14070.20055333906342873No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG13770.1962771484650614No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG13730.19570698971861242No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC13580.19356889441942873No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG13550.193141275359592No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT13520.19271365629975526No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA13160.18758222758171444No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG12600.1796000051314287No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA12030.17147524299453076No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11920.16990730644179605No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11820.1684819095756736No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG11680.16648635396310218No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11630.16577365553004095No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC11430.16292286179779605No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT11110.1583615918262042No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10930.15579587746718382No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC10800.1539428615412246No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10040.143109845358694No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT10010.14268222629885727No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9940.14168444849257156No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA9840.1402590516264491No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9720.13854857538710214No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT9580.13655301977453074No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC9340.13313206729583685No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG9280.13227682917616337No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG9240.1317066704297144No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG9170.1307088926234287No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT9050.12899841638408174No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT9050.12899841638408174No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9020.128570797324245No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8960.12771555920457153No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA8910.12700286077151032No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8880.12657524171167356No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8700.12400952735265316No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8670.12358190829281643No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA8600.12258413048653072No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8460.12058857487395927No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC8450.12044603518734703No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.12001841612751031No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC8380.11944825738106131No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8270.11788032082832663No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8180.11659746364881642No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8090.11531460646930622No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC7890.11246381273706132No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7700.10975555869142865No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7700.10975555869142865No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7580.10804508245208172No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7520.10718984433240825No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC7420.1057644474662858No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7410.10562190777967356No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7380.10519428871983683No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7310.10419651091355112No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT7170.10220095530097967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC200.001841447737.08
GTGCTAG251.2331353E-437.027
CTCGTAT352.3869909E-531.71428736
TCGTATG352.3869909E-531.71428737
TGTGCTA303.5980617E-430.83333226
AACTCTC303.5980617E-430.83333219
GTAACCA303.5980617E-430.83333233
CCGGGTC250.005494901429.614
TAGACCG250.005494901429.65
CAAATTA502.718225E-729.630
TCCACAA250.005494901429.626
ATGTTCG250.005494901429.623
TTGTTAC1000.027.75000215
ATTCAAA556.255905E-726.9090927
CGGGCCG556.255905E-726.9090917
GATTCAA556.255905E-726.9090926
AGATCTT358.865935E-426.4285728
AACCACG358.865935E-426.42857235
ATAGATC358.865935E-426.4285726
AACGCAC358.865935E-426.42857232
Produced by FastQC (version 0.11.5)