##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633491.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	701559
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.785740044671936	33.0	33.0	33.0	27.0	33.0
2	31.90715534972825	33.0	33.0	33.0	33.0	33.0
3	31.63006247514464	33.0	33.0	33.0	27.0	33.0
4	31.912916803861116	33.0	33.0	33.0	33.0	33.0
5	32.02393954036653	33.0	33.0	33.0	33.0	33.0
6	35.145785315276406	37.0	37.0	37.0	33.0	37.0
7	35.33066213960622	37.0	37.0	37.0	33.0	37.0
8	35.45964060043418	37.0	37.0	37.0	33.0	37.0
9	35.579681822911546	37.0	37.0	37.0	33.0	37.0
10	35.562340444638295	37.0	37.0	37.0	33.0	37.0
11	35.58839384855728	37.0	37.0	37.0	33.0	37.0
12	35.57768341650524	37.0	37.0	37.0	33.0	37.0
13	35.58454670241562	37.0	37.0	37.0	33.0	37.0
14	35.559741946151355	37.0	37.0	37.0	33.0	37.0
15	35.57806827365909	37.0	37.0	37.0	33.0	37.0
16	35.55645070478748	37.0	37.0	37.0	33.0	37.0
17	35.55994007631575	37.0	37.0	37.0	33.0	37.0
18	35.54416948538897	37.0	37.0	37.0	33.0	37.0
19	35.533605869214135	37.0	37.0	37.0	33.0	37.0
20	35.51341369720865	37.0	37.0	37.0	33.0	37.0
21	35.51576702743461	37.0	37.0	37.0	33.0	37.0
22	35.40502794490556	37.0	37.0	37.0	33.0	37.0
23	35.46344641006672	37.0	37.0	37.0	33.0	37.0
24	35.45037979699498	37.0	37.0	37.0	33.0	37.0
25	35.47663703266582	37.0	37.0	37.0	33.0	37.0
26	35.35557522603231	37.0	37.0	37.0	33.0	37.0
27	35.370732896306656	37.0	37.0	37.0	33.0	37.0
28	35.37951049020823	37.0	37.0	37.0	33.0	37.0
29	35.37645871551787	37.0	37.0	37.0	33.0	37.0
30	35.35724436576254	37.0	37.0	37.0	33.0	37.0
31	35.33231274917719	37.0	37.0	37.0	33.0	37.0
32	35.31783356781112	37.0	37.0	37.0	33.0	37.0
33	35.31160600890303	37.0	37.0	37.0	33.0	37.0
34	35.27310889034279	37.0	37.0	37.0	33.0	37.0
35	35.181557075028614	37.0	37.0	37.0	33.0	37.0
36	35.191948218182645	37.0	37.0	37.0	33.0	37.0
37	35.16459200152803	37.0	37.0	37.0	33.0	37.0
38	35.13520031814858	37.0	37.0	37.0	33.0	37.0
39	35.01958637833739	37.0	37.0	37.0	27.0	37.0
40	34.76712578699725	37.0	37.0	37.0	27.0	37.0
41	34.92117412790656	37.0	37.0	37.0	27.0	37.0
42	34.90531658777095	37.0	37.0	37.0	27.0	37.0
43	34.52549821183963	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	24.0
16	41.0
17	36.0
18	27.0
19	31.0
20	44.0
21	144.0
22	360.0
23	805.0
24	1681.0
25	3018.0
26	5006.0
27	7582.0
28	10560.0
29	14528.0
30	19043.0
31	24389.0
32	30886.0
33	39922.0
34	57080.0
35	112632.0
36	373715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79283709566836	20.5073272525903	13.42481530420107	23.275020347540266
2	16.734016668590954	22.058871741364587	38.74143158308852	22.46568000695594
3	17.716542728409156	29.42118909457366	29.417340523035122	23.44492765398206
4	11.383361912540499	18.539424339221647	41.2206243523353	28.856589395902553
5	13.125481962315359	37.75790774546403	35.02413909592778	14.092471196292827
6	26.48986043939284	42.54952185062126	17.03406270890973	13.926555001076174
7	26.021760108558222	32.77172696808109	23.18137177343602	18.025141149924668
8	24.211791167955937	34.226059390585824	20.285820579594873	21.276328861863362
9	27.023814105442305	13.987134367886378	20.21198502192973	38.77706650474158
10	16.501249360353157	26.38908488095798	32.74364664981847	24.366019108870386
11	33.397476192308844	24.61247022702296	23.31407622167202	18.675977358996178
12	23.806978457977163	27.336546177869575	28.035560801016025	20.82091456313724
13	28.24680461657537	23.17780828127071	25.309489294556837	23.26589780759708
14	22.473234610346385	21.463198391012018	28.862861142113495	27.200705856528103
15	25.757634069265734	27.38714776661692	26.409753135516755	20.445465028600587
16	21.73088792246981	28.027863657938962	25.989261060010634	24.25198735958059
17	22.062435233529897	26.439116310958877	25.79013311781333	25.7083153376979
18	22.64741810738655	26.03173788662108	29.072394481433495	22.248449524558875
19	25.734400100347937	24.920070870732182	28.78760018758223	20.55792884133765
20	24.798199438678715	24.37357371226084	29.204386231236434	21.623840617824015
21	22.351790797352752	27.02210362920296	29.139815753201088	21.4862898202432
22	21.99544158082214	26.71336266800084	28.64349256441725	22.647703186759774
23	22.08039523404304	26.67972330196035	30.128756098916842	21.111125365079772
24	22.58028191499218	27.627469678245163	26.703669969311207	23.08857843745145
25	22.511150166985242	26.981764897891697	29.38697956978672	21.120105365336343
26	22.647703186759774	27.329846812598795	29.056572576219537	20.965877424421894
27	22.682910489353	26.805300765865738	28.27602525233088	22.235763492450385
28	21.32165648220606	26.717781398285815	29.85322688469537	22.10733523481275
29	24.789504517795365	26.301280434004838	27.423495386703046	21.485719661496752
30	23.521328926006223	25.471414378548346	29.47278846112729	21.53446823431814
31	22.74847874519463	26.09759122183594	28.826228442654145	22.327701590315282
32	20.435772329910957	25.94478867778761	29.66521703805382	23.95422195424761
33	20.501768204812425	26.187106145028427	30.61567166838427	22.695453981774875
34	21.99430126332924	26.01805407670631	29.729787516089168	22.257857143875285
35	22.02209650221863	27.276822049179046	28.385068112589245	22.31601333601308
36	23.563378133556835	26.736596636918637	27.870072224859204	21.829953004665324
37	22.570019057556102	25.961465821121244	28.17453699546296	23.293978125859695
38	22.494473023651608	26.64522869780018	27.701732854970146	23.15856542357806
39	21.743003795831857	26.4079001195908	28.846469078153085	23.002627006424266
40	21.816269194750546	28.15158810591839	28.973899557984435	21.05824314134663
41	22.33383079683961	25.644315018409003	28.47158970236288	23.55026448238851
42	20.900594247953485	28.13633635945088	28.524757005469247	22.438312387126384
43	22.76373049166214	25.915140422972264	28.116808422385002	23.20432066298059
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	72.0
2	101.0
3	446.5
4	792.0
5	792.0
6	1024.0
7	1256.0
8	1226.5
9	1197.0
10	1662.5
11	2128.0
12	2128.0
13	3318.5
14	4509.0
15	7548.5
16	10588.0
17	11121.5
18	11655.0
19	11655.0
20	8845.5
21	6036.0
22	4362.5
23	2689.0
24	3140.0
25	3591.0
26	3591.0
27	4777.5
28	5964.0
29	7522.5
30	9081.0
31	12873.5
32	16666.0
33	16666.0
34	25479.0
35	34292.0
36	37295.0
37	40298.0
38	44662.5
39	49027.0
40	49027.0
41	53441.5
42	57856.0
43	61644.5
44	65433.0
45	64860.0
46	64287.0
47	64287.0
48	63267.5
49	62248.0
50	62792.5
51	63337.0
52	59894.0
53	56451.0
54	56451.0
55	52969.0
56	49487.0
57	39376.5
58	29266.0
59	25972.5
60	22679.0
61	22679.0
62	18820.5
63	14962.0
64	10901.0
65	6840.0
66	5568.0
67	4296.0
68	4296.0
69	3321.5
70	2347.0
71	1735.5
72	1124.0
73	931.5
74	739.0
75	739.0
76	457.0
77	175.0
78	115.0
79	55.0
80	46.0
81	37.0
82	37.0
83	27.0
84	17.0
85	10.5
86	4.0
87	3.5
88	3.0
89	3.0
90	2.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	701559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.9045835665889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43006939539303	13.881648831856296
2	9.565739202121703	3.6167263224541037
3	3.8424313186009815	2.179186918841119
4	2.1795603705084643	1.6481472465081093
5	1.41723755878356	1.3396142931866128
6	1.1173305990112767	1.267360180830931
7	0.8298479861550561	1.0981551421283637
8	0.6908774986463839	1.044860112594918
9	0.5475328347806545	0.9315792207505977
>10	4.828083890905448	19.105993661164938
>50	0.7714968400731959	10.265123086140184
>100	0.6817528283735768	25.995778020869555
>500	0.0746609845232125	9.665334571766886
>1k	0.02187038940578952	5.958821190098958
>5k	0.0015083027176406565	2.0016712008084356
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8069	1.150152731274205	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5971	0.851104468761715	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2970	0.4233428692383677	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2119	0.30204159593134716	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1938	0.27624191265453085	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1883	0.2684022298908574	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1858	0.2648387377255512	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1648	0.2349054035369798	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1646	0.2346203241637553	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1601	0.22820603826620425	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1546	0.2203663555025308	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1449	0.20654000590114302	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1407	0.20055333906342873	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1377	0.1962771484650614	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1373	0.19570698971861242	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1358	0.19356889441942873	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1355	0.193141275359592	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1352	0.19271365629975526	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1316	0.18758222758171444	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1260	0.1796000051314287	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	1203	0.17147524299453076	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	1192	0.16990730644179605	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1182	0.1684819095756736	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	1168	0.16648635396310218	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1163	0.16577365553004095	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	1143	0.16292286179779605	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	1111	0.1583615918262042	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	1093	0.15579587746718382	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	1080	0.1539428615412246	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1004	0.143109845358694	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	1001	0.14268222629885727	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	994	0.14168444849257156	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	984	0.1402590516264491	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	972	0.13854857538710214	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	958	0.13655301977453074	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	934	0.13313206729583685	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	928	0.13227682917616337	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	924	0.1317066704297144	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG	917	0.1307088926234287	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	905	0.12899841638408174	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	905	0.12899841638408174	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	902	0.128570797324245	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	896	0.12771555920457153	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	891	0.12700286077151032	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	888	0.12657524171167356	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	870	0.12400952735265316	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	867	0.12358190829281643	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	860	0.12258413048653072	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	846	0.12058857487395927	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	845	0.12044603518734703	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.12001841612751031	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	838	0.11944825738106131	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	827	0.11788032082832663	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	818	0.11659746364881642	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	809	0.11531460646930622	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	789	0.11246381273706132	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	770	0.10975555869142865	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	770	0.10975555869142865	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	758	0.10804508245208172	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	752	0.10718984433240825	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	742	0.1057644474662858	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	741	0.10562190777967356	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	738	0.10519428871983683	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	731	0.10419651091355112	No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT	717	0.10220095530097967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.27619059836735E-4	0.0	0.0
2	1.4253968661224502E-4	0.0	4.27619059836735E-4	0.0	0.0
3	1.4253968661224502E-4	0.0	9.97777806285715E-4	0.0	0.0
4	1.4253968661224502E-4	0.0	0.0018530159259591852	0.0	0.0
5	1.4253968661224502E-4	0.0	0.0027082540456326554	0.0	0.0
6	1.4253968661224502E-4	0.0	0.005273968404653066	1.4253968661224502E-4	0.0
7	2.8507937322449004E-4	0.0	0.011688254302204092	1.4253968661224502E-4	0.0
8	4.27619059836735E-4	0.0	0.01254349242187756	1.4253968661224502E-4	0.0
9	4.27619059836735E-4	0.0	0.01567936552734695	8.5523811967347E-4	0.0
10	0.001567936552734695	0.0	0.017389841766693892	8.5523811967347E-4	0.0
11	0.0018530159259591852	0.0	0.024374286410693898	0.0012828571795102052	0.0
12	0.0018530159259591852	0.0	0.026084762650040838	0.0014253968661224502	0.0
13	0.0018530159259591852	0.0	0.028222857949224512	0.0014253968661224502	0.0
14	0.0018530159259591852	0.0	0.03093111199485717	0.00171047623934694	0.0
15	0.0018530159259591852	0.0	0.0329266676074286	0.0018530159259591852	0.0
16	0.0018530159259591852	0.0	0.034779683533387784	0.0018530159259591852	0.0
17	0.0018530159259591852	0.0	0.03506476290661227	0.0018530159259591852	1.4253968661224502E-4
18	0.00199555561257143	0.0	0.035920001026285744	0.0021380952991836752	1.4253968661224502E-4
19	0.00199555561257143	0.0	0.03634762008612248	0.0022806349857959203	1.4253968661224502E-4
20	0.00199555561257143	0.0	0.03691777883257146	0.0027082540456326554	1.4253968661224502E-4
21	0.00199555561257143	0.0	0.03805809632546942	0.0037060318519183703	1.4253968661224502E-4
22	0.00199555561257143	0.0	0.03848571538530615	0.004703809658204086	1.4253968661224502E-4
23	0.00199555561257143	0.0	0.03848571538530615	0.005701587464489801	1.4253968661224502E-4
24	0.00199555561257143	0.0	0.0386282550719184	0.008980000256571436	1.4253968661224502E-4
25	0.00199555561257143	0.0	0.038770794758530645	0.009977778062857151	1.4253968661224502E-4
26	0.00199555561257143	0.0	0.03905587413175513	0.010833016182530622	1.4253968661224502E-4
27	0.00199555561257143	0.0	0.03919841381836738	0.01282857179510205	1.4253968661224502E-4
28	0.00199555561257143	0.0	0.039340953504979626	0.0357774613396735	1.4253968661224502E-4
29	0.00199555561257143	0.0	0.039340953504979626	0.09022762162555109	1.4253968661224502E-4
30	0.00199555561257143	0.0	0.039340953504979626	0.15793397276636748	1.4253968661224502E-4
31	0.00199555561257143	0.0	0.039340953504979626	0.2752441348482451	1.4253968661224502E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGATC	20	0.0018414477	37.0	8
GTGCTAG	25	1.2331353E-4	37.0	27
CTCGTAT	35	2.3869909E-5	31.714287	36
TCGTATG	35	2.3869909E-5	31.714287	37
TGTGCTA	30	3.5980617E-4	30.833332	26
AACTCTC	30	3.5980617E-4	30.833332	19
GTAACCA	30	3.5980617E-4	30.833332	33
CCGGGTC	25	0.0054949014	29.6	14
TAGACCG	25	0.0054949014	29.6	5
CAAATTA	50	2.718225E-7	29.6	30
TCCACAA	25	0.0054949014	29.6	26
ATGTTCG	25	0.0054949014	29.6	23
TTGTTAC	100	0.0	27.750002	15
ATTCAAA	55	6.255905E-7	26.90909	27
CGGGCCG	55	6.255905E-7	26.90909	17
GATTCAA	55	6.255905E-7	26.90909	26
AGATCTT	35	8.865935E-4	26.428572	8
AACCACG	35	8.865935E-4	26.428572	35
ATAGATC	35	8.865935E-4	26.428572	6
AACGCAC	35	8.865935E-4	26.428572	32
>>END_MODULE